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Literature summary for 2.3.1.180 extracted from
- Reaction mechanism of recombinant 3-oxoacyl-(acyl-carrier-protein) synthase III from Cuphea wrightii embryo, a fatty acid synthase type II condensing enzyme (2000), Biochem. J., 345, 153-160.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Cuphea wrightii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C111A | site-directed mutagenesis, the mutant enzyme loses all condensing activity | Cuphea wrightii |
| C111S | site-directed mutagenesis, the mutant enzyme loses all condensing activity, but is still able to bind acetyl-CoA and malonyl-ACP and to decarboxylate the latter to acetyl-ACP | Cuphea wrightii |
| C111S/H261A | site-directed mutagenesis, the mutant enzyme loses all condensing activity | Cuphea wrightii |
| C111S/H261R | site-directed mutagenesis, the mutant enzyme loses all condensing activity, but is still able to bind acetyl-CoA and malonyl-ACP and to decarboxylate the latter to acetyl-ACP | Cuphea wrightii |
| H261A | site-directed mutagenesis, the mutant enzyme loses all condensing activity | Cuphea wrightii |
| H261R | site-directed mutagenesis, the mutant enzyme shows reduced condensing activity compared to the wild-type enzyme | Cuphea wrightii |
| R150A | site-directed mutagenesis, the mutant enzyme shows reduced condensing activity compared to the wild-type enzyme | Cuphea wrightii |
| R266A | site-directed mutagenesis, the mutant enzyme shows reduced condensing activity compared to the wild-type enzyme | Cuphea wrightii |
| R306A | site-directed mutagenesis, the mutant enzyme shows highly reduced condensing activity compared to the wild-type enzyme | Cuphea wrightii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Cuphea wrightii | |
| 0.0014 | - |
acetyl-CoA | pH 7.6, 30°C, recombinant His-tagged wild-type enzyme | Cuphea wrightii |  |
| 0.0041 | - |
malonyl-ACP | pH 7.6, 30°C, recombinant His-tagged wild-type enzyme | Cuphea wrightii | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + malonyl-[acyl-carrier protein] | Cuphea wrightii | - |
acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cuphea wrightii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) | Cuphea wrightii |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| acetyl-CoA + a malonyl-[acyl-carrier protein] = an acetoacetyl-[acyl-carrier protein] + CoA + CO2 | ping pong reaction mechanism, catalysis involves His261, Arg150, and Arg306, determination of substrate binding site | Cuphea wrightii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | - |
Cuphea wrightii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.2 | - |
purified recombinant His-tagged mutant R306A | Cuphea wrightii |
| 0.8 | - |
purified recombinant His-tagged mutant H261R | Cuphea wrightii |
| 1.9 | - |
purified recombinant His-tagged mutant R150A | Cuphea wrightii |
| 3.5 | - |
purified recombinant His-tagged mutant R266A | Cuphea wrightii |
| 7 | - |
purified recombinant His-tagged wild-type enzyme | Cuphea wrightii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Cuphea wrightii | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-oxoacyl-[acyl-carrier-protein] synthase III | - |
Cuphea wrightii |
| ACP synthase III | - |
Cuphea wrightii |
| fatty acid synthase type II condensing enzyme | - |
Cuphea wrightii |
| KAS III | - |
Cuphea wrightii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Cuphea wrightii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Cuphea wrightii |
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