Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in strain JM109, coexpression with gene phaC from Aeromonas caviae and gene phaC1 from Pseudomonas sp. strain 61-3 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
F87A | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain confers the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli confers the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
F87C | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain confers the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli confers the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
F87D | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain does not confer the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli does not confer the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
F87E | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain does not confer the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli does not confer the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
F87G | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain does not confer the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli does not confer the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
F87H | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain does not confer the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli does not confer the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
F87I | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain confers the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli confers the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
F87K | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain does not confer the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli does not confer the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
F87L | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain confers the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli confers the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
F87M | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain confers the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli confers the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
F87N | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain does not confer the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli does not confer the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
F87P | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain does not confer the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli does not confer the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
F87Q | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain confers the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli does not confer the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
F87R | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain does not confer the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli does not confer the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
F87S | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain confers the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli confers the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
F87T | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain confers the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli confers the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
F87V | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain confers the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli does not confer the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
F87W | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain confers the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli confers the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
F87Y | site-directed mutagenesis, coexpression of the mutant enzyme with the phaC gene from Aeromonas caviae in a recombinant strain confers the ability to synthesize polyhydroxyalkanoates, coexpression of the mutant with gene phaC1 from Pseudomonas sp. strain 61-3 in Escherichia coli confers the ability to synthesize polyhydroxyalkanoates, overview | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | the wild-type enzyme is able to induce synthesis of polyhydroxyalkanoates PHA copolymers from glucose in a strain which is transformed with gene phaC from Aeromonas caviae or gene phaC1 from Pseudomonas sp. strain 61-3, thus fatty acid biosynthesis may supply monomers for PHA production, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene fabH | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the wild-type enzyme is able to induce synthesis of polyhydroxyalkanoates PHA copolymers from glucose in a strain which is transformed with gene phaC from Aeromonas caviae or gene phaC1 from Pseudomonas sp. strain 61-3, thus fatty acid biosynthesis may supply monomers for PHA production, overview | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-ketoacyl-ACP synthase III | - |
Escherichia coli |
FabH | - |
Escherichia coli |