Cloned (Comment) | Organism |
---|---|
genes fabH1 and fabH2, expression of His-tagged enzymes in Escherichia coli strain BL21(DE3) | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
genes fabH1 and fabH2 encoding isozymes FabH1 and FabH2 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificities of FabH1 and FabH2 | Bacillus subtilis |
0.024 | - |
purified recombinant His-tagged isoyzme FabH1, primer substrate acetyl-CoA | Bacillus subtilis |
0.105 | - |
purified recombinant His-tagged isoyzme FabH2, primer substrate acetyl-CoA | Bacillus subtilis |
0.155 | - |
purified recombinant His-tagged isoyzme FabH1, primer substrate 2-methylbutyryl-CoA | Bacillus subtilis |
0.162 | - |
purified recombinant His-tagged isoyzme FabH2, primer substrate isovaleryl-CoA | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methylbutyryl-CoA + malonyl-[acyl-carrier protein] | 2-methylbutyryl-CoA is the preferred primer substrate of isozyme FabH1 | Bacillus subtilis | 3-oxo-4-methyl-hexanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Bacillus subtilis | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
butyryl-CoA + malonyl-[acyl-carrier protein] | - |
Bacillus subtilis | 3-oxo-hexanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
heptanoyl-CoA + malonyl-[acyl-carrier protein] | - |
Bacillus subtilis | 3-oxo-nonanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
hexanoyl-CoA + malonyl-[acyl-carrier protein] | - |
Bacillus subtilis | 3-oxo-octanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
isobutyryl-CoA + malonyl-[acyl-carrier protein] | - |
Bacillus subtilis | 3-oxo-4-methyl-pentanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
isovaleryl-CoA + malonyl-[acyl-carrier protein] | isovaleryl-CoA is the preferred primer substrate of isozyme FabH2 | Bacillus subtilis | 3-oxo-5-methyl-hexanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
additional information | substrate specificities of FabH1 and FabH2 with different primer substrates, both isozymes prefer branched-chain fatty acids | Bacillus subtilis | ? | - |
? | |
octanoyl-CoA + malonyl-[acyl-carrier protein] | low activity with octanoyl-CoA as primer substrate of isozyme FabH1, no activity with FabH2 | Bacillus subtilis | 3-oxo-decanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
propionyl-CoA + malonyl-[acyl-carrier protein] | - |
Bacillus subtilis | 3-oxo-valeryl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
valeryl-CoA + malonyl-[acyl-carrier protein] | - |
Bacillus subtilis | 3-oxo-heptanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-ketoacyl-acyl carrier protein synthase III | - |
Bacillus subtilis |
FabH | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Bacillus subtilis |