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Literature summary for 2.3.1.180 extracted from
- Characterization of beta-ketoacyl-acyl carrier protein synthase III from Streptomyces glaucescens and its role in initiation of fatty acid biosynthesis (1998), J. Bacteriol., 180, 4481-4486.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the recombinant enzyme is useful for production of fatty acids by directed biosynthesis profiting of the flexible substrate specificity for acyl-CoAs of the enzyme, overview | Streptomyces glaucescens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene fabH, overexpression of the His-tagged in Escherichia coli | Streptomyces glaucescens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| thiolactomycin | inhibits both KAS III and ACAT activities | Streptomyces glaucescens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0004 | - |
isobutyryl-CoA | pH 7.0, 30°C, recombinant His-tagged enzyme | Streptomyces glaucescens | |
| 0.0007 | - |
butyryl-CoA | pH 7.0, 30°C, recombinant His-tagged enzyme | Streptomyces glaucescens | |
| 0.0024 | - |
acetyl-CoA | pH 7.0, 30°C, recombinant His-tagged enzyme | Streptomyces glaucescens | |
| 0.0037 | - |
malonyl-[acyl-carrier protein] | pH 7.0, 30°C, recombinant His-tagged enzyme | Streptomyces glaucescens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
2 * 37000, recombinant His-tagged enzyme, SDS-PAGE | Streptomyces glaucescens |
| 72000 | - |
recombinant His-tagged enzyme, gel filtration | Streptomyces glaucescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + malonyl-[acyl-carrier protein] | Streptomyces glaucescens | - |
acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| butyryl-CoA + malonyl-[acyl-carrier protein] | Streptomyces glaucescens | - |
3-oxo-hexanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| isobutyryl-CoA + malonyl-[acyl-carrier protein] | Streptomyces glaucescens | - |
3-oxo-4-methyl-pentanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| additional information | Streptomyces glaucescens | the enzyme is involved in biosynthesis of straight-chain and branched-chain fatty acids with the specificity for acyl-CoA substrate determining the produced fatty acid spectrum | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces glaucescens | - |
gene fabH | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Streptomyces glaucescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Streptomyces glaucescens | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| butyryl-CoA + malonyl-[acyl-carrier protein] | - |
Streptomyces glaucescens | 3-oxo-hexanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| isobutyryl-CoA + malonyl-[acyl-carrier protein] | - |
Streptomyces glaucescens | 3-oxo-4-methyl-pentanoyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
| additional information | the enzyme is involved in biosynthesis of straight-chain and branched-chain fatty acids with the specificity for acyl-CoA substrate determining the produced fatty acid spectrum | Streptomyces glaucescens | ? | - |
? | |
| additional information | the enzyme shows also acyl-CoA:[acyl-carrier-protein] transacylase activity, i.e. ACAT activity, in a ratio of 1:0.12 with beta-oxoacyl-[acyl-carrier-protein] synthase III activity | Streptomyces glaucescens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 37000, recombinant His-tagged enzyme, SDS-PAGE | Streptomyces glaucescens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-ketoacyl-acyl carrier protein synthase III | - |
Streptomyces glaucescens |
| beta-ketoacyl-[acyl-carrier-protein] synthase III | - |
Streptomyces glaucescens |
| FabH | - |
Streptomyces glaucescens |
| KAS III | - |
Streptomyces glaucescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Streptomyces glaucescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | 7.7 | recombinant His-tagged enzyme | Streptomyces glaucescens |
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