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Literature summary for 2.3.1.18 extracted from

  • Musso, R.E.; Zabin, I.
    Substrate specificity and kinetic studies on thiogalactoside transacetylase (1973), Biochemistry, 12, 553-557.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline weak Escherichia coli
acetyl-CoA
-
Escherichia coli
CoA
-
Escherichia coli
MgSO4 1 mM, addition to 5,5'-dithiobis(2-nitrobenzoic acid) assay mixture lacking EDTA inhibits, preincubation of transacetylase (2 mg/ml in 0.05 M Tris, pH 7.8) with MgSO4 for 1 h at 30°C and subsequent dilution of the enzyme 1000fold in 5,5'-dithiobis(2-nitrobenzoic acid) assay mixture lacking both EDTA and the metal ion stimulates Escherichia coli
MnSO4 40% inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
acetyl-CoA
-
Escherichia coli
770
-
isopropyl beta-D-thiogalactoside
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
MgSO4 1 mM, addition to 5,5'-dithiobis(2-nitrobenzoic acid) assay mixture lacking EDTA inhibits, preincubation of transacetylase (2 mg/ml in 0.05 M Tris, pH 7.8) with MgSO4 for 1 h at 30°C and subsequent dilution of the enzyme 1000-fold in 5,5'-dithiobis(2-nitrobenzoic acid) assay mixture lacking both EDTA and the metal ion stimulates Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
A324-5
-
Escherichia coli A324-5
-
A324-5
-

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + a beta-D-galactoside = CoA + a 6-acetyl-beta-D-galactoside ordered bi-bi reaction mechanism with acetyl-CoA and CoA as the first substrate and final product, respectively Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + isopropyl beta-D-thiogalactoside
-
Escherichia coli CoA + isopropyl 6-O-acetyl-beta-D-thiogalactoside
-
?
acetyl-CoA + isopropyl beta-D-thiogalactoside
-
Escherichia coli A324-5 CoA + isopropyl 6-O-acetyl-beta-D-thiogalactoside
-
?
acetyl-CoA + o-nitrophenyl-beta-D-galactoside
-
Escherichia coli CoA + o-nitrophenyl 6-O-acetyl-beta-D-galactoside
-
?
acetyl-CoA + o-nitrophenyl-beta-D-thiogalactoside
-
Escherichia coli CoA + o-nitrophenyl 6-O-acetyl-beta-D-thiogalactoside
-
?
acetyl-CoA + p-nitrophenyl-beta-D-galactoside twice as effective as isopropyl-beta-D-thiogalactoside Escherichia coli CoA + p-nitrophenyl 6-O-acetyl-beta-D-galactoside
-
?
acetyl-CoA + p-nitrophenyl-beta-D-galactoside twice as effective as isopropyl-beta-D-thiogalactoside Escherichia coli A324-5 CoA + p-nitrophenyl 6-O-acetyl-beta-D-galactoside
-
?
acetyl-CoA + p-nitrophenyl-beta-D-glucoside
-
Escherichia coli CoA + p-nitrophenyl-6-O-acetyl-beta-D-glucoside
-
?
acetyl-CoA + p-nitrophenyl-beta-D-glucoside
-
Escherichia coli A324-5 CoA + p-nitrophenyl-6-O-acetyl-beta-D-glucoside
-
?
acetyl-CoA + p-nitrophenyl-beta-D-lactoside
-
Escherichia coli CoA + p-nitrophenyl 6-O-acetyl-beta-D-lactoside
-
?
acetyl-CoA + p-nitrophenyl-beta-D-thiogalactoside
-
Escherichia coli CoA + p-nitrophenyl 6-O-acetyl-beta-D-thiogalactoside
-
?
additional information crotonyl-CoA Escherichia coli ?
-
?
additional information oleoyl-CoA Escherichia coli ?
-
?
additional information crotonyl-CoA Escherichia coli A324-5 ?
-
?
additional information oleoyl-CoA Escherichia coli A324-5 ?
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.057
-
acetyl-CoA
-
Escherichia coli
0.068
-
CoA
-
Escherichia coli