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Literature summary for 2.3.1.18 extracted from

  • Zabin, I.
    Crystalline thiogalactoside transacetylase (1963), J. Biol. Chem., 238, 3300-3306.
    View publication on PubMed
Search for more literature for 2.3.1.18

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ stimulates when enzyme is initially incubated with the ion in Tris buffer but has no effect if initially incubated in phosphate or if added to the standard assay medium containing 0.05 M phosphate Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 ML308
-
Escherichia coli ML308
-
 ML308
-

Purification (Commentary)

Purification (Comment) Organism
strain ML308 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
35.3
-
 isopropyl 6-O-acetyl-beta-D-thiogalactoside produced Escherichia coli

Storage Stability

Storage Stability Organism
frozen, in salt solution of low ionic strength, stable Escherichia coli
in ammonium sulfate suspension, stable for months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + isopropyl beta-D-thiogalactoside
-
 Escherichia coli CoA + isopropyl 6-O-acetyl-beta-D-thiogalactoside
-
 ?
acetyl-CoA + isopropyl beta-D-thiogalactoside
-
 Escherichia coli ML308 CoA + isopropyl 6-O-acetyl-beta-D-thiogalactoside
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
56
-
 30 min, about 25% loss of activity Escherichia coli