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Literature summary for 2.3.1.175 extracted from

  • Scheidegger, A.; Gutzwiller, A.; Kuenzi, M.T.; Fiechter, A.; Nuesch, J.
    Investigation of acetyl-CoA:deacetylcephalosporin C O-acetyltransferase of Cephalosporium acremonium (1985), J. Biotechnol., 3, 109-117.
No PubMed abstract available
Search for more literature for 2.3.1.175

Inhibitors

Inhibitors Comment Organism Structure
2,6-dihydroxybenzoic acid 0.1 mM, 27% inhibition Acremonium chrysogenum
coenzyme A 0.1 mM, 61% inhibition Acremonium chrysogenum
deacetoxycephalosporin C 0.1 mM, 57% inhibition Acremonium chrysogenum
Penicillin N 0.1 mM, 14% inhibition Acremonium chrysogenum
pyruvate 0.1 mM, 37% inhibition Acremonium chrysogenum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 deacetylcephalosporin C
-
 Acremonium chrysogenum
0.1
-
 acetyl-CoA
-
 Acremonium chrysogenum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 gel filtration Acremonium chrysogenum

Organism

Organism UniProt Comment Textmining
Acremonium chrysogenum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Acremonium chrysogenum

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Acremonium chrysogenum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 0.0000378 mg cephalosporin C per min per mg Acremonium chrysogenum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + deacetylcephalosporin C
-
 Acremonium chrysogenum CoA + cephalosporin C
-
 ?

Synonyms

Synonyms Comment Organism
acetyl-CoA:deacetylcephalosporin C O-acetyltransferase
-
 Acremonium chrysogenum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Acremonium chrysogenum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 40 15°C: about 45% of maximal activity, 40°C: about 75% of maximal activity Acremonium chrysogenum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 8 no significant influence of pH-value on enzyme activity is measured after 20 min Acremonium chrysogenum

pI Value

Organism Comment pI Value Maximum pI Value
Acremonium chrysogenum isoelectric focusing
-
 4.3