Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant Ss5MaT1 in Escherichia coli | Salvia splendens |
Protein Variants | Comment | Organism |
---|---|---|
D171A | kinetic parameters, mutant with severely reduced activity | Salvia splendens |
D390A | kinetic parameters | Salvia splendens |
H167A | kinetic parameters, mutant with severely reduced activity | Salvia splendens |
K258A | kinetic parameters | Salvia splendens |
N315A | kinetic parameters, mutant with reduced activity | Salvia splendens |
Q150A | kinetic parameters | Salvia splendens |
R301A | kinetic parameters | Salvia splendens |
Y45A | kinetic parameters | Salvia splendens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoA-SH | product inhibition, competitive versus malonyl-CoA, noncompetitive versus shisonin | Salvia splendens | |
malonylshisonin | product inhibition, noncompetitive versus shisonin and malonyl-CoA | Salvia splendens | |
p-coumaric acid | dead-end inhibitor, competitive with respect to shisonin, uncompetitive with respect to malonyl-CoA | Salvia splendens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | effects of pH on kinetic parameters, kinetic mechanism | Salvia splendens | |
0.0054 | - |
shisonin | pH 7, 30°C, malonyl-CoA as cosubstrate, H167A mutant Ss5MaT1 | Salvia splendens | |
0.0064 | - |
malonyl-CoA | pH 7, 30°C, shisonin as cosubstrate, H167A mutant Ss5MaT1 | Salvia splendens | |
0.013 | - |
malonyl-CoA | pH 7, 30°C, shisonin as cosubstrate, Y45A mutant Ss5MaT1 | Salvia splendens | |
0.014 | - |
malonyl-CoA | pH 7, 30°C, shisonin as cosubstrate, Q150A mutant Ss5MaT1 | Salvia splendens | |
0.019 | - |
shisonin | pH 7, 30°C, malonyl-CoA as cosubstrate, Y45A mutant Ss5MaT1 | Salvia splendens | |
0.02 | - |
malonyl-CoA | pH 7, 30°C, shisonin as cosubstrate, K258A mutant Ss5MaT1 | Salvia splendens | |
0.022 | - |
malonyl-CoA | pH 7, 30°C, shisonin as cosubstrate, wild-type Ss5MaT1 | Salvia splendens | |
0.025 | - |
malonyl-CoA | pH 7, 30°C, shisonin as cosubstrate, N315A mutant Ss5MaT1 | Salvia splendens | |
0.035 | - |
shisonin | pH 7, 30°C, malonyl-CoA as cosubstrate, wild-type Ss5MaT1 | Salvia splendens | |
0.035 | - |
malonyl-CoA | pH 7, 30°C, shisonin as cosubstrate, R301A mutant Ss5MaT1 | Salvia splendens | |
0.039 | - |
shisonin | pH 7, 30°C, malonyl-CoA as cosubstrate, Q150A mutant Ss5MaT1 | Salvia splendens | |
0.044 | - |
shisonin | pH 7, 30°C, malonyl-CoA as cosubstrate, K258A mutant Ss5MaT1 | Salvia splendens | |
0.062 | - |
shisonin | pH 7, 30°C, malonyl-CoA as cosubstrate, N315A mutant Ss5MaT1 | Salvia splendens | |
0.071 | - |
malonyl-CoA | pH 7, 30°C, shisonin as cosubstrate, D171A mutant Ss5MaT1 | Salvia splendens | |
0.084 | - |
shisonin | pH 7, 30°C, malonyl-CoA as cosubstrate, R301A mutant Ss5MaT1 | Salvia splendens | |
0.49 | - |
shisonin | pH 7, 30°C, malonyl-CoA as cosubstrate, D171A mutant Ss5MaT1 | Salvia splendens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + pelargonidin 3-O-(6-caffeoyl-beta-D-glucoside) 5-O-beta-D-glucoside | Salvia splendens | involved in the late stage of biosynthesis of salvianin | CoA + 4'''-demalonylsalvianin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salvia splendens | Q8W1W9 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
malonyl-CoA + pelargonidin 3-O-(6-caffeoyl-beta-D-glucoside) 5-O-beta-D-glucoside = CoA + 4'''-demalonylsalvianin | reaction mechanism, kinetic mechanism | Salvia splendens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Salvia splendens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + pelargonidin 3-O-(6-caffeoyl-beta-D-glucoside) 5-O-beta-D-glucoside | involved in the late stage of biosynthesis of salvianin | Salvia splendens | CoA + 4'''-demalonylsalvianin | - |
? | |
malonyl-CoA + pelargonidin 3-O-(6-caffeoyl-beta-D-glucoside) 5-O-beta-D-glucoside | Ss5MaT1 catalyzes the regiospecific transfer of the malonyl group from malonyl-CoA to the 6'''-hydroxyl group of the 5-glycosyl moiety of anthocyanins, mechanism, both substrates and Ss5MaT1 form a complex before catalysis can occur, His-167, Asp-390 and Asn-315 are very important for catalytic activity, His-167 and Asp-390 appear to be involved in the general acid/base mechanism | Salvia splendens | CoA + 4'''-demalonylsalvianin | - |
? | |
malonyl-CoA + shisonin | Ss5MaT1 specifically catalyzes the malonylation of the 6'''-hydroxyl group of the 5-glucosyl moiety | Salvia splendens | CoA + malonylshisonin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
malonyl-CoA:anthocyanin 5-O-glucoside-6'''-O-malonyltransferase | - |
Salvia splendens |
More | member of the versatile plant acyltransferase family | Salvia splendens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Salvia splendens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
shisonin | below, pH 7, 30°C, malonyl-CoA as cosubstrate, D390A mutant Ss5MaT1 | Salvia splendens | |
0.002 | - |
shisonin | pH 7, 30°C, malonyl-CoA as cosubstrate, H167A mutant Ss5MaT1 | Salvia splendens | |
0.072 | - |
shisonin | pH 7, 30°C, malonyl-CoA as cosubstrate, D171A mutant Ss5MaT1 | Salvia splendens | |
0.1 | - |
shisonin | pH 7, 30°C, malonyl-CoA as cosubstrate, N315A mutant Ss5MaT1 | Salvia splendens | |
1 | - |
shisonin | pH 7, 30°C, malonyl-CoA as cosubstrate, Y45A mutant Ss5MaT1 | Salvia splendens | |
1.4 | - |
shisonin | pH 7, 30°C, malonyl-CoA as cosubstrate, R301A mutant Ss5MaT1 | Salvia splendens | |
5.3 | - |
shisonin | pH 7, 30°C, malonyl-CoA as cosubstrate, K258A mutant Ss5MaT1 | Salvia splendens | |
5.8 | - |
shisonin | pH 7, 30°C, malonyl-CoA as cosubstrate, Q150A mutant Ss5MaT1 | Salvia splendens | |
8.7 | - |
shisonin | pH 7, 30°C, malonyl-CoA as cosubstrate, wild-type Ss5MaT1 | Salvia splendens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Salvia splendens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
unstable above | Salvia splendens |