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Literature summary for 2.3.1.171 extracted from

  • Suzuki, H.; Nakayama, T.; Yonekura-Sakakibara, K.; Fukui, Y.; Nakamura, N.; Yamaguchi, M.A.; Tanaka, Y.; Kusumi, T.; Nishino, T.
    cDNA cloning, heterologous expressions, and functional characterization of malonyl-coenzyme A:anthocyanidin 3-O-glucoside-6"-O-malonyltransferase from dahlia flowers (2002), Plant Physiol., 130, 2142-2151.
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
agriculture the gene for anthocyanin malonyltransferase should be useful in the stabilization of anthocyanin pigments, which enable flower color modification in transgenic plants Dahlia pinnata

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli. Creation of transgenic Petunia hybrida harboring Dv3MaT cDNA Dahlia pinnata

Inhibitors

Inhibitors Comment Organism Structure
CoA 0.1 mM, 13% inhibition Dahlia pinnata
Cu2+ 0.1 mM, 20°C, 20 min, complete inhibition Dahlia pinnata
Fe2+ 0.1 mM, 64% inhibition Dahlia pinnata
Hg2+ 0.1 mM, 65% inhibition Dahlia pinnata
NEM 5 mM, 20°C, 20 min, complete inhibition Dahlia pinnata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0188
-
 malonyl-CoA pH 7.0, 30°C Dahlia pinnata
0.0467
-
 pelargonidin 3-O-beta-D-glucoside pH 7.0, 30°C Dahlia pinnata

Organism

Organism UniProt Comment Textmining
Dahlia pinnata Q8GSN8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Dahlia pinnata

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Dahlia pinnata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + pelargonidin 3-O-beta-D-glucoside
-
 Dahlia pinnata CoA + pelargonidin 3-O-(6-O-malonyl-beta-D-glucoside)
-
 ?

Synonyms

Synonyms Comment Organism
3MaT
-
 Dahlia pinnata
anthocyanin malonyltransferase
-
 Dahlia pinnata
malonyl-coenzyme A:anthocyanidin 3-O-glucoside-6"-O-malonyltransferase
-
 Dahlia pinnata

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.3
-
 malonyl-CoA pH 7.0, 30°C, value is obtained by measurement of formation of pelargonidin 3-O-(6'-O-malonyl-beta-D-glucoside) Dahlia pinnata
7.3
-
 pelargonidin 3-O-beta-D-glucoside pH 7.0, 30°C, value is obtained by measurement of formation of pelargonidin 3-O-(6'-O-malonyl-beta-D-glucoside) Dahlia pinnata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Dahlia pinnata