Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | corrinoid/iron-sulfur protein required | Methanosarcina thermophila | |
Fe | a Ni/Fe-S cluster of multienzyme CO dehydrogenase/acetyl-CoA synthase complex is the active site of acetyl-CoA cleavage and synthesis | Methanosarcina thermophila | |
Ni | a Ni/Fe-S cluster of multienzyme CO dehydrogenase/acetyl-CoA synthase complex is the active site of acetyl-CoA cleavage and synthesis | Methanosarcina thermophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina thermophila | P72021 | TM1 | - |
Methanosarcina thermophila TM1 | P72021 | TM1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CH3-CO-S-CoA + H+ + tetrahydromethanopterin | - |
Methanosarcina thermophila | CH3-tetrahydromethanopterin + CO + HS-CoA | - |
? | |
CH3-CO-S-CoA + H+ + tetrahydromethanopterin | - |
Methanosarcina thermophila TM1 | CH3-tetrahydromethanopterin + CO + HS-CoA | - |
? | |
CO + H2O | the multienzyme complex catalyses the reversible oxidation of CO to CO2 | Methanosarcina thermophila | CO2 + H+ + electron | - |
r | |
CO + H2O | the multienzyme complex catalyses the reversible oxidation of CO to CO2 | Methanosarcina thermophila TM1 | CO2 + H+ + electron | - |
r | |
additional information | the multienzyme complex catalyses the exchange between free CO and carbonyl group of acetyl-CoA, and the exchange between CoA and the CoA moiety of acetyl-CoA | Methanosarcina thermophila | ? | - |
? | |
additional information | the multienzyme complex catalyses the exchange between free CO and carbonyl group of acetyl-CoA, and the exchange between CoA and the CoA moiety of acetyl-CoA | Methanosarcina thermophila TM1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | alpha,beta,gamma,delta,epsilon, the enzyme complex is part of a five-subunit complex, the alpha and epsilon subunits are required for CO oxidation, the gamma end delta subunits constitute a corrinoid/iron-sulfur protein, the beta subunit harbors the Ni/Fe-S cluster, that is the active site of acetyl-CoA cleavage/synthesis, the interaction between the alpha,epsilon dimer and the beta subunit is necessary for breaking and forming the C-C bond of acetyl-CoA, SDS-PAGE | Methanosarcina thermophila |