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Literature summary for 2.3.1.167 extracted from

  • Lin, S.L.; Wei, T.; Lin, J.F.; Guo, L.Q.; Wu, G.P.; Wei, J.B.; Huang, J.J.; Ouyang, P.L.
    Bio-production of baccatin III, an important precursor of paclitaxel by a cost-effective approach (2018), Mol. Biotechnol., 60, 492-505 .
    View publication on PubMed
Search for more literature for 2.3.1.167

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Taxus mairei

Protein Variants

Protein Variants Comment Organism
D390H catalytic efficiency towards 10-deacetylbaccatin III and vinyl acetate is 96% of the wild-type value Taxus mairei
D390R catalytic efficiency towards 10-deacetylbaccatin III and vinyl acetate is improved by 2.3fold Taxus mairei
I43S catalytic efficiency towards 10-deacetylbaccatin III and vinyl acetate is improved by 2.1fold Taxus mairei
I43S/D390R catalytic efficiency towards 10-deacetylbaccatin III and vinyl acetate is improved by 3.3fold, compared to that of wild-type enzyme. Catalytic efficiency is 2.99fold higher than the catalytic efficiency of wild-type enzyme towards 10-deacetylbaccatin III and acetyl-CoA Taxus mairei
I43T catalytic efficiency towards 10-deacetylbaccatin III and vinyl acetate is improved by 2.1fold Taxus mairei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.046
-
 vinyl acetate mutant enzyme I43S/D390R, pH 7.2, 37°C Taxus mairei
0.052
-
 acetyl-CoA wild-type enzyme, pH 7.2, 37°C Taxus mairei
0.065
-
 vinyl acetate mutant enzyme D390R, pH 7.2, 37°C Taxus mairei
0.065
-
 vinyl acetate mutant enzyme I43T, pH 7.2, 37°C Taxus mairei
0.066
-
 vinyl acetate mutant enzyme I43S, pH 7.2, 37°C Taxus mairei
0.128
-
 vinyl acetate wild-type enzyme, pH 7.2, 37°C Taxus mairei
0.134
-
 vinyl acetate mutant enzyme D390H, pH 7.2, 37°C Taxus mairei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 SDS-PAGE Taxus mairei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + 10-deacetylbaccatin III Taxus mairei the enzyme is involved in taxol biosynthesis CoA + baccatin III
-
 ?

Organism

Organism UniProt Comment Textmining
Taxus mairei H9BIH8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Taxus mairei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 10-deacetylbaccatin III
-
 Taxus mairei CoA + baccatin III
-
 ?
acetyl-CoA + 10-deacetylbaccatin III the enzyme is involved in taxol biosynthesis Taxus mairei CoA + baccatin III
-
 ?
vinyl acetate + 10-deacetylbaccatin III
-
 Taxus mairei vinyl alcohol + baccatin III
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Taxus mairei

Synonyms

Synonyms Comment Organism
DBAT
-
 Taxus mairei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0101
-
 acetyl-CoA wild-type enzyme, pH 7.2, 37°C Taxus mairei
0.0224
-
 vinyl acetate wild-type enzyme, pH 7.2, 37°C Taxus mairei
0.0224
-
 vinyl acetate mutant enzyme D390H, pH 7.2, 37°C Taxus mairei
0.0236
-
 vinyl acetate mutant enzyme I43T, pH 7.2, 37°C Taxus mairei
0.0244
-
 vinyl acetate mutant enzyme I43S, pH 7.2, 37°C Taxus mairei
0.0259
-
 vinyl acetate mutant enzyme D390R, pH 7.2, 37°C Taxus mairei
0.0263
-
 vinyl acetate mutant enzyme I43S/D390R, pH 7.2, 37°C Taxus mairei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 optimal pH for mutant enzymes I43S, and I43T and for wild-type enzyme Taxus mairei
6.5
-
 optimal pH for mutant enzymes D390H, D390R and I43S/D390R Taxus mairei

General Information

General Information Comment Organism
metabolism the enzyme is involved in taxol biosynthesis Taxus mairei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.168
-
 vinyl acetate mutant enzyme D390H, pH 7.2, 37°C Taxus mairei
0.174
-
 vinyl acetate wild-type enzyme, pH 7.2, 37°C Taxus mairei
0.193
-
 acetyl-CoA wild-type enzyme, pH 7.2, 37°C Taxus mairei
0.365
-
 vinyl acetate mutant enzyme I43T, pH 7.2, 37°C Taxus mairei
0.37
-
 vinyl acetate mutant enzyme I43S, pH 7.2, 37°C Taxus mairei
0.4
-
 vinyl acetate mutant enzyme D390R, pH 7.2, 37°C Taxus mairei
0.576
-
 vinyl acetate mutant enzyme I43S/D390R, pH 7.2, 37°C Taxus mairei