Application | Comment | Organism |
---|---|---|
biotechnology | optimizing the semi-biosynthetic method in bacteria potentially provides a practical means of developing commercial-scale production of baccatin III analogs, which serve as key intermediates in the semi-synthesis of second-generation taxols | Taxus sp. |
synthesis | bioengineered Escherichia coli cells synthesize nonnatural 10-deacetyl-10-acylbaccatin III derivatives as potential precursors of second-generation Taxol derivatives using the enzyme in concert with acyl-CoA transferases, determination of optimal alkanoic acid concentration for in vivo production of baccatin III analogues, overview | Taxus sp. |
Cloned (Comment) | Organism |
---|---|
gene dbat, functional expression in Escherichia coli strain JM109, co-expression with acetyl-CoA synthase, gene acs, EC 6.2.1.1, or with acetoacetate:acetyl-CoA CoA-transferase, genes atoAD of the ato operon, EC 2.8.3.8, expressed from and regulated by genes encoded on the bacterial chromosome, overview | Taxus sp. |
into the vector pCWori+ for expression in Escherichia coli JM109 and BL21DE3 cells | Taxus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 10-deacetylbaccatin III | Taxus sp. | - |
CoA + baccatin III | - |
? | |
butyryl-CoA + 10-deacetylbaccatin III | Taxus sp. | - |
CoA + 10-deacetyl-10-butyrylbaccatin III | - |
? | |
propionyl-CoA + 10-deacetylbaccatin III | Taxus sp. | - |
CoA + 10-deacetyl-10-propionylbaccatin III | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Taxus sp. | - |
- |
- |
Taxus sp. | - |
Taxus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 10-deacetylbaccatin III | - |
Taxus sp. | CoA + baccatin III | - |
? | |
butyryl-CoA + 10-deacetylbaccatin III | - |
Taxus sp. | CoA + 10-deacetyl-10-butyrylbaccatin III | - |
? | |
additional information | DBAT catalyzes the transfer of acetyl, propionyl or n-butyryl from CoA to the C10-hydroxyl of 10-deacetylbaccatin III | Taxus sp. | ? | - |
? | |
n-butyryl-CoA + 10-deacetylbaccatin III | - |
Taxus sp. | CoA + 10-deacetyl-10-butyrylbaccatin III | - |
? | |
propionyl-CoA + 10-deacetylbaccatin III | - |
Taxus sp. | CoA + 10-deacetyl-10-propionylbaccatin III | - |
? |
Synonyms | Comment | Organism |
---|---|---|
10-deacetylbaccatin III 10-O-acetyltransferase | - |
Taxus sp. |
10-deacetylbaccatin III 10beta-O-acetyltransferase | - |
Taxus sp. |
DBAT | - |
Taxus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Taxus sp. |