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Literature summary for 2.3.1.167 extracted from
- Expression cloned cDNA for 10-deacetylbaccatin III-10-O-acetyltransferase in Escherichia coli: a comparative study of three fusion systems (2004), Protein Expr. Purif., 35, 17-24.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli. Three kinds of fusion systems with isopropyl-beta-D-1-thiogalactopyranoside inducible promoters are used. The fusion partners are glutathione S-transferase tag, hexahistidine fusion tag, and a biotinylated peptide tag | Taxus baccata |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0085 | - |
acetyl-CoA | recombinant enzyme with a hexahistidine fusion tag | Taxus baccata |  |
| 0.011 | - |
10-deacetylbaccatin III | recombinant enzyme with a hexahistidine fusion tag | Taxus baccata | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Taxus baccata | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Taxus baccata |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.1 | - |
recombinant enzyme with biotinylated peptide tag | Taxus baccata |
| 16.1 | - |
recombinant glutathione S-transferase-fusion protein | Taxus baccata |
| 24.4 | - |
recombinant enzyme with a hexahistidine fusion tag | Taxus baccata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + 10-deacetylbaccatin III | - |
Taxus baccata | CoA + baccatin III | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 10-DABT | - |
Taxus baccata |
| 10-deacetylbaccatin III-10-O-acetyltransferase | - |
Taxus baccata |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Taxus baccata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
recombinant enzymes | Taxus baccata |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | half-maximal aactivity of recombinant enzyme at pH 6.0 and 8.0 | Taxus baccata |
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Release 2023.1 (January 2023)
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