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Literature summary for 2.3.1.165 extracted from

  • Spencer, J.B.; Jordan, P.M.
    Purification and properties of 6-methylsalicylic acid synthase from Penicillium patulum (1992), Biochem. J., 288, 839-846.
No PubMed abstract available

General Stability

General Stability Organism
PMSF and benzamidine in disruption buffer extend half-life from 20 min to 6 h, 15% glycerol and proteinase inhibitors further improve stability Penicillium griseofulvum

Inhibitors

Inhibitors Comment Organism Structure
1,3-dibromo-propane-2-one 0.02 mM, complete inactivation after 40 min, acetyl-CoA protects Penicillium griseofulvum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
malonyl-CoA
-
Penicillium griseofulvum
0.01
-
acetyl-CoA
-
Penicillium griseofulvum
0.012
-
NADPH
-
Penicillium griseofulvum
0.065
-
acetoacetyl-CoA
-
Penicillium griseofulvum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
alpha4, 4 * 180000, SDS-PAGE Penicillium griseofulvum
750000
-
gel filtration Penicillium griseofulvum

Organism

Organism UniProt Comment Textmining
Penicillium griseofulvum
-
NRRL 2159A
-
Penicillium griseofulvum
-
also known as Penicillium urticae
-
Penicillium griseofulvum NRRL 2159A
-
NRRL 2159A
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, poly(ethylene glycol) 6000, DEAE-Sepharose, hydroxyapatite, Mono Q Penicillium griseofulvum

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + 3 malonyl-CoA + NADPH + H+ = 6-methylsalicylate + 4 CoA + 3 CO2 + NADP+ + H2O a multienzyme complex with a 4'-phosphopantetheine prosthetic group on the acyl carrier protein, it has a similar sequence to vertebrate type I fatty acid synthase, acetoacetyl-CoA can also act as a starter molecule Penicillium griseofulvum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.245
-
-
Penicillium griseofulvum

Storage Stability

Storage Stability Organism
-70°C, ammonium sulfate precipitate, loss of 5% activity per month Penicillium griseofulvum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetoacetyl-CoA + malonyl-CoA + NADPH
-
Penicillium griseofulvum ?
-
?
acetoacetyl-CoA + malonyl-CoA + NADPH
-
Penicillium griseofulvum NRRL 2159A ?
-
?
acetyl-CoA + malonyl-CoA exclusive product in the absence of NADPH, 5:1 ratio of 6-methylsalicylic acid to triacetic acid lactone at 0.0005 mM NADPH, less than 1% triacetic acid lactone at 0.012 mM NADPH Penicillium griseofulvum triacetic lactone
-
?
acetyl-CoA + malonyl-CoA exclusive product in the absence of NADPH, 5:1 ratio of 6-methylsalicylic acid to triacetic acid lactone at 0.0005 mM NADPH, less than 1% triacetic acid lactone at 0.012 mM NADPH Penicillium griseofulvum NRRL 2159A triacetic lactone
-
?
acetyl-CoA + malonyl-CoA + NADPH
-
Penicillium griseofulvum 6-methylsalicylate + CoA + CO2 + NADP+
-
?
acetyl-CoA + malonyl-CoA + NADPH
-
Penicillium griseofulvum NRRL 2159A 6-methylsalicylate + CoA + CO2 + NADP+
-
?

Subunits

Subunits Comment Organism
tetramer alpha4, 4 * 180000, SDS-PAGE Penicillium griseofulvum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
Penicillium griseofulvum

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9
-
Penicillium griseofulvum