Cloned (Comment) | Organism |
---|---|
pPenIAT and pPenIATC103S are used to express the penDE gene in Penicillium chrysogenum, pPBCalphabeta, pDEC103S and palphabeta-DEC103S are used for expression in Escherichia coli | Penicillium chrysogenum |
Protein Variants | Comment | Organism |
---|---|---|
C103S | unprocessed inactive variant of isopenicillin N acyltransferase | Penicillium chrysogenum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Penicillium chrysogenum | 5777 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
11000 | - |
processed protein, subunit alpha, determined by SDS-PAGE and Western blot analysis | Penicillium chrysogenum |
29000 | - |
processed protein, subunit beta, determined by SDS-PAGE and Western blot analysis | Penicillium chrysogenum |
40000 | - |
isopenicillin N acyltransferase precursor, proacyltransferase, proIAT, determined by SDS-PAGE and Western blot analysis | Penicillium chrysogenum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenylacetyl-CoA + isopenicillin N + H2O | Penicillium chrysogenum | - |
CoA + penicillin G + L-2-aminohexanedioate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium chrysogenum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
cell extracts of Penicillium chrysogenum are prepared, Escherichia coli soluble extracts are used for in vitro IAT activity measurement | Penicillium chrysogenum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Penicillium chrysogenum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenylacetyl-CoA + isopenicillin N + H2O | - |
Penicillium chrysogenum | CoA + penicillin G + L-2-aminohexanedioate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | - |
Penicillium chrysogenum |
Synonyms | Comment | Organism |
---|---|---|
IAT | - |
Penicillium chrysogenum |
isopenicillin N acyltransferase | - |
Penicillium chrysogenum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
26 | - |
activity assay | Penicillium chrysogenum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
activity assay | Penicillium chrysogenum |