BRENDA - Enzyme Database
show all sequences of 2.3.1.160

The molecular architecture of major enzymes from ajmaline biosynthetic pathway

Stoeckigt, J.; Panjikar, S.; Ruppert, M.; Barleben, L.; Ma, X.; Loris, E.; Hill, M.; Phytochem. Rev. 6, 15-34 (2007)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Rauvolfia serpentina
Crystallization (Commentary)
Crystallization (Commentary)
Organism
using the hanging-drop vapour-diffusion method
Rauvolfia serpentina
Engineering
Protein Variants
Commentary
Organism
D164A
reduction in enzyme activity
Rauvolfia serpentina
D32A
reduction in enzyme activity
Rauvolfia serpentina
D362A
reduction in enzyme activity
Rauvolfia serpentina
H160A
reduction in enzyme activity
Rauvolfia serpentina
S29A
reduction in enzyme activity
Rauvolfia serpentina
Organism
Organism
UniProt
Commentary
Textmining
Rauvolfia serpentina
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
of the recombinant protein by his-tag affinity column chromatography
Rauvolfia serpentina
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
16-epi-vellosimine + acetyl-CoA
VS responsible for generation of the basic carbon skeleton of the target compound ajmaline, His160 and Asp164 are the most important amino acids for the catalytic process, His-X-X-X-Asp sequence together with Asp-Phe-Gly-Trp-Gly are highly conserved motifs in acyltransferases
682286
Rauvolfia serpentina
vinorine + CoA
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
vinorine synthase
a member of the BAHD enzyme super-family, benzylalcohol acetyl-, anthocyanin-O-hydroxy-cinnamoyl-, anthranilate-N-hydroxy-cinnamoyl-benzoyl- and deacetylvindoline acetyl transferase, involved in synthesis of ajmaline
Rauvolfia serpentina
VS
-
Rauvolfia serpentina
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Rauvolfia serpentina
Crystallization (Commentary) (protein specific)
Crystallization
Organism
using the hanging-drop vapour-diffusion method
Rauvolfia serpentina
Engineering (protein specific)
Protein Variants
Commentary
Organism
D164A
reduction in enzyme activity
Rauvolfia serpentina
D32A
reduction in enzyme activity
Rauvolfia serpentina
D362A
reduction in enzyme activity
Rauvolfia serpentina
H160A
reduction in enzyme activity
Rauvolfia serpentina
S29A
reduction in enzyme activity
Rauvolfia serpentina
Purification (Commentary) (protein specific)
Commentary
Organism
of the recombinant protein by his-tag affinity column chromatography
Rauvolfia serpentina
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
16-epi-vellosimine + acetyl-CoA
VS responsible for generation of the basic carbon skeleton of the target compound ajmaline, His160 and Asp164 are the most important amino acids for the catalytic process, His-X-X-X-Asp sequence together with Asp-Phe-Gly-Trp-Gly are highly conserved motifs in acyltransferases
682286
Rauvolfia serpentina
vinorine + CoA
-
-
-
?
Other publictions for EC 2.3.1.160
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
682286
Stoeckigt
-
The molecular architecture of ...
Rauvolfia serpentina
Phytochem. Rev.
6
15-34
2007
-
-
1
1
5
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
5
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
662350
Ma
Crystal structure of vinorine ...
Rauvolfia serpentina
J. Biol. Chem.
280
13576-13583
2005
-
-
1
1
-
-
-
-
-
-
-
1
-
3
-
-
1
1
-
-
-
-
2
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
661320
Gerasimenko
Purification and partial amino ...
Rauvolfia serpentina x Rhazya stricta
Bioorg. Med. Chem.
12
2781-2786
2004
-
-
-
-
-
-
-
2
-
-
2
1
-
5
-
-
1
-
-
3
1
-
4
1
3
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
1
-
-
-
1
-
3
1
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
661321
Bayer
Acetyltransfer in natural prod ...
Rauvolfia serpentina x Rhazya stricta
Bioorg. Med. Chem.
12
2787-2795
2004
-
-
1
-
13
-
7
9
-
-
1
1
-
7
-
-
1
1
-
-
-
-
4
1
3
1
-
-
7
1
-
-
-
-
1
-
-
-
1
-
-
13
-
-
7
-
9
-
-
1
1
-
-
-
1
-
-
-
-
4
1
1
-
-
7
1
-
-
1
-
-
-
-
-
-
486439
Pfitzner
-
Properties of vinorine synthas ...
Rauvolfia serpentina
Z. Naturforsch. C
41
103-114
1986
-
-
-
-
-
-
-
3
-
-
1
1
-
1
-
-
1
-
-
-
1
-
2
-
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
1
-
-
-
1
-
-
1
-
2
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
486440
Pfitzner
-
Biogenetic link between sarpag ...
Rauvolfia serpentina
Tetrahedron Lett.
24
5197-5200
1983
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-