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Literature summary for 2.3.1.16 extracted from

  • Zeng, J.; Li, D.
    Expression and purification of His-tagged rat mitochondrial 3-ketoacyl-CoA thiolase wild-type and His352 mutant proteins (2004), Protein Expr. Purif., 35, 320-326.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged wild-type and His352 mutant proteins overexpressed in Escherichia coli Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
H352A 3.3fold increase in KM-value for acetoacetyl-CoA compared to wild-type value, Vmax is decreased 1154 times Rattus norvegicus
H352E 2.7fold increase in KM-value for acetoacetyl-CoA compared to wild-type value, Vmax is decreased 1250 times Rattus norvegicus
H352K 3.7fold increase in KM-value for acetoacetyl-CoA compared to wild-type value, Vmax is decreased 526 times Rattus norvegicus
H352Y 3.1fold increase in KM-value for acetoacetyl-CoA compared to wild-type value, Vmax is decreased 429 times Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
acetoacetyl-CoA pH 8.2, wild-type enzyme Rattus norvegicus
0.058
-
acetoacetyl-CoA pH 8.2, mutant enzyme H352E Rattus norvegicus
0.065
-
acetoacetyl-CoA pH 8.2, mutant enzyme H352Y Rattus norvegicus
0.07
-
acetoacetyl-CoA pH 8.2, mutant enzyme H352A Rattus norvegicus
0.079
-
acetoacetyl-CoA pH 8.2, mutant enzyme H352K Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus key enzyme involved in fatty acid oxidation ?
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
-80°C, wild-type and mutant enzymes H352E, H352A, H352K and H352Y, stable for at least 3 months Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA + acetoacetyl-CoA
-
Rattus norvegicus acetyl-CoA + acetyl-CoA
-
?
additional information key enzyme involved in fatty acid oxidation Rattus norvegicus ?
-
?

Synonyms

Synonyms Comment Organism
3-ketoacyl-CoA thiolase
-
Rattus norvegicus