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Literature summary for 2.3.1.16 extracted from
- Characterization of two 3-ketothiolases possessing differing substrate specificities in the polyhydroxyalkanoate synthesizing organism Alcaligenes eutrophus (1988), FEMS Microbiol. Lett., 52, 91-96.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CoA | inhibition of condensation | Cupriavidus necator |  |
| N-ethylmaleimide | - |
Cupriavidus necator | |
| NAD+ | - |
Cupriavidus necator | |
| NADP+ | - |
Cupriavidus necator | |
| Semicarbazide | - |
Cupriavidus necator | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.093 | - |
CoA | - |
Cupriavidus necator | |
| 0.394 | - |
acetoacetyl-CoA | - |
Cupriavidus necator | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
4 * 46000, SDS-PAGE | Cupriavidus necator |
| 168000 | - |
gel filtration, sucrose density gradient centrifugation | Cupriavidus necator |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cupriavidus necator | - |
basonym Alcaligenes eutrophus | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cupriavidus necator |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA | stoichiometry | Cupriavidus necator |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CoA + 3-oxoacyl-CoA | - |
Cupriavidus necator | acyl-CoA + acetyl-CoA | - |
r | |
| CoA + 3-oxodecanoyl-CoA | - |
Cupriavidus necator | acetyl-CoA + octanoyl-CoA | - |
r | |
| CoA + 3-oxoheptanoyl-CoA | - |
Cupriavidus necator | acetyl-CoA + pentanoyl-CoA | - |
r | |
| CoA + 3-oxohexanoyl-CoA | - |
Cupriavidus necator | acetyl-CoA + butanoyl-CoA | - |
? | |
| CoA + 3-oxooctanoyl-CoA | - |
Cupriavidus necator | acetyl-CoA + hexanoyl-CoA | - |
r | |
| CoA + 3-oxopentanoyl-CoA | - |
Cupriavidus necator | acetyl-CoA + propanoyl-CoA | - |
r | |
| CoA + acetoacetyl-CoA | - |
Cupriavidus necator | acetyl-CoA + acetyl-CoA | - |
r | |
| additional information | active with all 3-oxoacyl-CoAs (C4-C10) tested | Cupriavidus necator | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 46000, SDS-PAGE | Cupriavidus necator |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 6.4 | Cupriavidus necator |
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Release 2023.1 (January 2023)
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