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Literature summary for 2.3.1.158 extracted from

  • Chellamuthu, M.; Kumaresan, K.; Subramanian, S.; Muthumanickam, H.
    Functional analysis of sesame diacylglycerol acyltransferase and phospholipid diacylglycerol acyltransferase genes using in silico and in vitro approaches (2019), Plant Mol. Biol. Rep., 37, 146-156 .
No PubMed abstract available
Search for more literature for 2.3.1.158

Cloned(Commentary)

Cloned (Comment) Organism
gene SiPDAT, DNA and amino acid sequence determination and analysis, genetic structure, phylogenetic analysis, quantitative real-time PCR expression analysis, recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 and complementation, higher oil content in SiPDAT gene-transformed mutants, lipid analysis, overview Sesamum indicum
gene SiPDAT, DNA and amino acid sequence determination and analysis, genetic structure, phylogenetic analysis, quantitative real-time PCR expression analysis, recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 and complementation, higher oil content in SiPDAT gene-transformed mutants, SiPDAT1-expressing mutants have higher polyunsaturated (C18:1; C18:2) fatty acid content, lipid analysis, overview Sesamum indicum

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane
-
 Sesamum indicum 5789
-
lipid droplet
-
 Sesamum indicum 5811
-
soluble SiPDAT2 does not have any transmembrane domains Sesamum indicum
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phospholipid + 1,2-diacyl-sn-glycerol Sesamum indicum
-
 lysophospholipid + triacylglycerol
-
 ?

Organism

Organism UniProt Comment Textmining
Sesamum indicum XP_011088820
-
-
Sesamum indicum XP_020553631
-
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Sesamum indicum
-
additional information tissue distribution analysis by quantitative real-time PCR. SiPDAT1 expression is highest at flowering stage and developing seeds Sesamum indicum
-
additional information tissue distribution analysis by quantitative real-time PCR. SiPDAT2 expression is highest in stem and developing seeds Sesamum indicum
-
seed developing Sesamum indicum
-
stem
-
 Sesamum indicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phospholipid + 1,2-diacyl-sn-glycerol
-
 Sesamum indicum lysophospholipid + triacylglycerol
-
 ?

Subunits

Subunits Comment Organism
? x * 73872, PDAT1, sequence calculation Sesamum indicum
? x * 76594, PDAT2, sequence calculation Sesamum indicum

Synonyms

Synonyms Comment Organism
PDAT
-
 Sesamum indicum
PDAT2
-
 Sesamum indicum
phospholipid:diacylglycerol acyltransferase
-
 Sesamum indicum
SiPDAT
-
 Sesamum indicum
SiPDAT2
-
 Sesamum indicum

pI Value

Organism Comment pI Value Maximum pI Value
Sesamum indicum sequence calculation
-
 8.11
Sesamum indicum sequence calculation
-
 8.5

General Information

General Information Comment Organism
metabolism specific role of DGAT (EC 2.3.1.20) and PDAT (EC 2.3.1.158) genes in fatty acid biosynthesis Sesamum indicum
physiological function phospholipid:diacylglycerol acyltransferase (PDAT) is an acyl-CoA-independent pathway enzyme using phosphatidylcholine (PC) as the acyl donor, in which the transfer of an acyl group from the sn-2 position of phosphatidylcholine to the sn-3 position of DAG yields triacylglycerol (TAG) Sesamum indicum