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Literature summary for 2.3.1.158 extracted from
- Characterisation of phospholipid diacylglycerol acyltransferases (PDATs) from Camelina sativa and their roles in stress responses (2017), Biol. Open, 6, 1024-1034 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in tobacco leaves. The gene shows phospholipid diacylglycerol acyltransferase enzymatic activity and substantially increased triacylglycerol accumulation in the leaves | Camelina sativa |
| expression in tobacco leaves. The gene shows phospholipid diacylglycerol acyltransferase enzymatic activity and substantially increased triacylglycerol accumulation in the leaves. CsPDAT1-A shows a higher preference for alpha-linolenic acid (18:3 omega-3) | Camelina sativa |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65953 | - |
calculation from sequence, CsPDAT1-B | Camelina sativa |
| 73627 | - |
calculation from sequence, CsPDAT2-A | Camelina sativa |
| 73721 | - |
calculation from sequence, CsPDAT2-B | Camelina sativa |
| 74301 | - |
calculation from sequence, CsPDAT1-A | Camelina sativa |
| 74321 | - |
calculation from sequence, CsPDAT1-C | Camelina sativa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Camelina sativa | XP_010419957.1 | - |
- |
| Camelina sativa | XP_010453452.1 | - |
- |
| Camelina sativa | XP_010492131.1 | - |
- |
| Camelina sativa | XP_010503132.1 | - |
- |
| Camelina sativa | XP_010514811.1 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | CsPDAT1-C preferentially accumulates in flower and leaf tissues | Camelina sativa | - |
| flower | low activity, CsPDAT1-A | Camelina sativa | - |
| flower | low activity, CsPDAT1-B | Camelina sativa | - |
| flower | low activity, CsPDAT2-A | Camelina sativa | - |
| flower | low activity, CsPDAT2-B | Camelina sativa | - |
| leaf | CsPDAT1-C preferentially accumulates in flower and leaf tissues | Camelina sativa | - |
| leaf | low activity, CsPDAT1-A | Camelina sativa | - |
| leaf | low activity, CsPDAT1-B | Camelina sativa | - |
| leaf | low activity, CsPDAT2-A | Camelina sativa | - |
| leaf | low activity, CsPDAT2-B | Camelina sativa | - |
| additional information | different members of Camelina sativa phospholipid diacylglycerol acyltransferase family contribute to triacylglycerol synthesis in different tissues | Camelina sativa | - |
| root | high expression of CsPDAT2-A is detected in stem and root tissues | Camelina sativa | - |
| root | high expression of CsPDAT2-B is detected in stem and root tissues | Camelina sativa | - |
| root | low activity, CsPDAT1-A | Camelina sativa | - |
| root | low activity, CsPDAT1-B | Camelina sativa | - |
| root | low activity, CsPDAT1-C | Camelina sativa | - |
| seed | CsPDAT1-A is mainly expressed in seeds | Camelina sativa | - |
| seed | intermediate activity, CsPDAT1-C | Camelina sativa | - |
| seed | low activity, CsPDAT1-B | Camelina sativa | - |
| seed | low activity, CsPDAT2-A | Camelina sativa | - |
| seed | low activity, CsPDAT2-B | Camelina sativa | - |
| stem | high expression of CsPDAT2-A is detected in stem and root tissues | Camelina sativa | - |
| stem | high expression of CsPDAT2-B is detected in stem and root tissues | Camelina sativa | - |
| stem | low activity, CsPDAT1-A | Camelina sativa | - |
| stem | low activity, CsPDAT1-B | Camelina sativa | - |
| stem | low activity, CsPDAT1-C | Camelina sativa | - |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CsPDAT1-A | - |
Camelina sativa |
| CsPDAT1-B | - |
Camelina sativa |
| CsPDAT1-C | - |
Camelina sativa |
| CsPDAT2-A | - |
Camelina sativa |
| CsPDAT2-B | - |
Camelina sativa |
| PDAT | - |
Camelina sativa |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Camelina sativa | calculated from sequence, CsPDAT1-B | - |
6.05 |
| Camelina sativa | calculated from sequence, CsPDAT2-B | - |
6.05 |
| Camelina sativa | calculated from sequence, CsPDAT1-A | - |
6.33 |
| Camelina sativa | calculated from sequence, CsPDAT1-C | - |
6.43 |
| Camelina sativa | calculated from sequence, CsPDAT2-A | - |
8.56 |
| Camelina sativa | calculated from sequence, CsPDAT2-B | - |
8.61 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Camelina sativa | drought treatment results in a reduction of CsPDAT2-B expression | down |
| Camelina sativa | cold stress induces upregulation of CsPDAT1-A expression by 3.5fold compared to the control | up |
| Camelina sativa | cold stress induces upregulation of CsPDAT1-C expression by 2.5fold compared to the control | up |
| Camelina sativa | drought treatment results in an enhancement of CsPDAT2-A mRNAs by twofold | up |
| Camelina sativa | osmotic stress upregulates the expression of CsPDAT1-C by 3.3fold | up |
| Camelina sativa | salt stress leads to an increase in CsPDAT2-B transcript by 5.1fold | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | different members of Camelina sativa phospholipid diacylglycerol acyltransferase family are involved in different types of stress responses in camelina seedlings, providing evidence of their roles in oil biosynthesis and regulation in camelina vegetative tissue | Camelina sativa |
| metabolism | the enzyme catalyses the final acylation step in triacylglycerol biosynthesis by transferring a fatty acyl moiety from a phospholipid to diacylglycerol | Camelina sativa |
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