BRENDA - Enzyme Database
show all sequences of 2.3.1.158

Identification of a pair of phospholipid:diacylglycerol acyltransferases from developing flax (Linum usitatissimum L.) seed catalyzing the selective production of trilinolenin

Pan, X.; Siloto, R.M.; Wickramarathna, A.D.; Mietkiewska, E.; Weselake, R.J.; J. Biol. Chem. 288, 24173-24188 (2013)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
alpha-linolenic acid
stimulation; stimulation
Linum usitatissimum
Cloned(Commentary)
Commentary
Organism
expression in Saccharomyces cerevisiae; expression in Saccharomyces cerevisiae
Linum usitatissimum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
76890
-
x * 76890, calculated
Linum usitatissimum
79072
-
x * 79072, calculated
Linum usitatissimum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Linum usitatissimum
V5LWK6
-
-
Linum usitatissimum
V5LY10
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
low expression in seed
Linum usitatissimum
-
seed
preferential expression
Linum usitatissimum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
linolenoyl-phosphatidylcholine + 1,2-dilinolenoylglycerol
-
736422
Linum usitatissimum
lyso-phosphatidylcholine + trilinolenoylglycerol
-
-
-
?
additional information
in the presence of alpha-linolenic acid, isoform PDAT1 produces triacylglycerol with a specific fatty acid composition. PDAT1 has the ability to synthesize trilinolenin, which is the major molecular species of triacylglycerol in flax oil
736422
Linum usitatissimum
?
-
-
-
-
additional information
in the presence of alpha-linolenic acid, isoform PDAT2 produces triacylglycerol with a specific fatty acid composition. PDAT2 has the ability to synthesize trilinolenin, which is the major molecular species of triacylglycerol in flax oil
736422
Linum usitatissimum
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 76890, calculated; x * 79072, calculated
Linum usitatissimum
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Linum usitatissimum
calculated
-
6.4
Linum usitatissimum
calculated
-
8.2
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
alpha-linolenic acid
stimulation
Linum usitatissimum
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Saccharomyces cerevisiae
Linum usitatissimum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
76890
-
x * 76890, calculated
Linum usitatissimum
79072
-
x * 79072, calculated
Linum usitatissimum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
low expression in seed
Linum usitatissimum
-
seed
preferential expression
Linum usitatissimum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
linolenoyl-phosphatidylcholine + 1,2-dilinolenoylglycerol
-
736422
Linum usitatissimum
lyso-phosphatidylcholine + trilinolenoylglycerol
-
-
-
?
additional information
in the presence of alpha-linolenic acid, isoform PDAT1 produces triacylglycerol with a specific fatty acid composition. PDAT1 has the ability to synthesize trilinolenin, which is the major molecular species of triacylglycerol in flax oil
736422
Linum usitatissimum
?
-
-
-
-
additional information
in the presence of alpha-linolenic acid, isoform PDAT2 produces triacylglycerol with a specific fatty acid composition. PDAT2 has the ability to synthesize trilinolenin, which is the major molecular species of triacylglycerol in flax oil
736422
Linum usitatissimum
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 76890, calculated
Linum usitatissimum
?
x * 79072, calculated
Linum usitatissimum
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Linum usitatissimum
calculated
-
6.4
Linum usitatissimum
calculated
-
8.2
General Information
General Information
Commentary
Organism
physiological function
expression of isoform PDAT1 restores triacylglycerol synthesis in Saccharomyces cerevisiae H1246 when culturing yeast in the presence of alpha-linolenic acid; expression of isoform PDAT1 restores triacylglycerol synthesis in Saccharomyces cerevisiae H1246 when culturing yeast in the presence of alpha-linolenic acid
Linum usitatissimum
General Information (protein specific)
General Information
Commentary
Organism
physiological function
expression of isoform PDAT1 restores triacylglycerol synthesis in Saccharomyces cerevisiae H1246 when culturing yeast in the presence of alpha-linolenic acid
Linum usitatissimum
Other publictions for EC 2.3.1.158
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736102
Hung
Functional study of diacylglyc ...
Chlamydomonas reinhardtii
FEBS Lett.
587
2364-2370
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
-
736422
Pan
Identification of a pair of ph ...
Linum usitatissimum
J. Biol. Chem.
288
24173-24188
2013
1
-
1
-
-
-
-
-
-
-
2
-
-
5
-
-
-
-
-
2
-
-
4
1
-
-
-
-
-
-
-
-
-
2
-
2
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
4
2
-
-
-
-
-
-
-
2
-
1
2
-
-
-
736985
Fan
Dual role for phospholipid:dia ...
Arabidopsis thaliana
Plant Cell
25
3506-3518
2013
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
720694
Yoon
Phospholipid:diacylglycerol ac ...
Chlamydomonas reinhardtii
Plant Cell
24
3708-3724
2012
-
2
1
-
-
-
-
-
2
-
3
1
-
2
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
-
-
2
1
-
-
-
-
-
-
-
-
2
-
3
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
1
1
3
3
1
-
-
721119
Zhang
Three diacylglycerol acyltrans ...
Yarrowia lipolytica, Yarrowia lipolytica ATCC 20362
Yeast
29
25-38
2012
-
-
1
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
720737
van Erp
Castor phospholipid:diacylglyc ...
Ricinus communis
Plant Physiol.
155
683-693
2011
-
1
1
-
-
-
-
-
1
-
-
1
-
3
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697014
Dauk
-
The role of diacylglycerol acy ...
Arabidopsis thaliana
Botany
87
552-560
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
684576
Arabolaza
Multiple pathways for triacylg ...
Streptomyces coelicolor
Appl. Environ. Microbiol.
74
2573-2582
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687725
Czabany
Structural and biochemical pro ...
Saccharomyces cerevisiae
J. Biol. Chem.
283
17065-17074
2008
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688089
Hernandez
The utilization and desaturati ...
Olea europaea
J. Exp. Bot.
59
2425-2435
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685341
Ghosal
Saccharomyces cerevisiae phosp ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1771
1457-1463
2007
-
1
1
-
-
-
-
-
1
-
2
-
-
3
-
1
1
-
-
-
5
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
1
1
-
-
5
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676641
Mhaske
Isolation and characterization ...
Arabidopsis thaliana
Plant Physiol. Biochem.
43
413-417
2005
-
-
-
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660241
Stahl
Cloning and functional charact ...
Arabidopsis thaliana
Plant Physiol.
135
1324-1335
2004
-
-
1
-
1
-
-
-
1
-
-
1
-
6
-
1
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
1
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
393081
Banas
The involvement of phospholipi ...
Arabidopsis thaliana, Crepis palaestina, Euphorbia lagascae, Ricinus communis
Biochem. Soc. Trans.
28
703-705
2000
-
-
-
-
-
-
-
-
-
-
-
4
-
9
-
-
-
-
-
4
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
4
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
393082
Dahlqvist
Phospholipid:diacylglycerol ac ...
Crepis palaestina, Helianthus annuus, Ricinus communis, Saccharomyces cerevisiae
Proc. Natl. Acad. Sci. USA
97
6487-6492
2000
-
-
1
-
-
-
-
-
-
-
-
4
-
10
-
-
-
-
-
3
-
-
24
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
3
-
-
24
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-