BRENDA - Enzyme Database
show all sequences of 2.3.1.158

Saccharomyces cerevisiae phospholipid:diacylglycerol acyl transferase (PDAT) devoid of its membrane anchor region is a soluble and active enzyme retaining its substrate specificities

Ghosal, A.; Banas, A.; Stahl, U.; Dahlqvist, A.; Lindqvist, Y.; Stymne, S.; Biochim. Biophys. Acta 1771, 1457-1463 (2007)

Data extracted from this reference:

Application
Application
Commentary
Organism
molecular biology
the conversion of a membrane bound lipid metabolizing enzyme into a soluble and active form might be applied to other enzymes with a membrane anchor region.
Saccharomyces cerevisiae
Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli, expression in Pichia pastoris of a N-terminal deleted version of PDAT, lacking the predicted membrane-spanning region under the control of the methanol inducible alcohol oxidase promoter
Saccharomyces cerevisiae
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
soluble form is produced for comparison with the native membrane bound enzyme
Saccharomyces cerevisiae
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
67000
-
SDS-PAGE, after deglycosylation
Saccharomyces cerevisiae
80000
-
SDS-PAGE
Saccharomyces cerevisiae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
extensive deglycosylation severely decreases the activity
Saccharomyces cerevisiae
Purification (Commentary)
Commentary
Organism
the recombinant protein by Ni2+ affinity chromatography
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00138
-
sn-1,2-distearoylphosphatidylethanolamine as substrate using the membrane bound enzyme
Saccharomyces cerevisiae
0.001399
-
sn-1,2-dipalmitoylphosphatidylethanolamine as substrate using the membrane bound enzyme
Saccharomyces cerevisiae
0.002453
-
sn-1,2-dilinolenoylphosphatidylethanolamine as substrate using the membrane bound enzyme
Saccharomyces cerevisiae
0.002468
-
sn-1,2-dioleoylphosphatidylethanolamine as substrate using the membrane bound enzyme
Saccharomyces cerevisiae
0.002597
-
sn-1,2-dilinoleoylphosphatidylethanolamine as substrate using the membrane bound enzyme
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
rac-1,2-divernoleoylglycerol + 1,3-dioleoyl-sn-glycerol
-
685341
Saccharomyces cerevisiae
1-oleoyl-sn-glycerol + rac-1,2-divernoleoyl-3-oleoylglycerol
-
-
-
?
sn-1,2-dioleoylglycerol + sn-1,2-dioleoylglycerol
-
685341
Saccharomyces cerevisiae
trioleoylglycerol + sn-1-oleoylglycerol
-
-
-
?
sn-1,2-dioleoylphosphatidylcholine + sn-1,2-dioleoylglycerol
-
685341
Saccharomyces cerevisiae
trioleoylglycerol + sn-1-oleoylphosphatidylcholine
-
-
-
?
sn-1,2-dioleoylphosphatidylcholine + sn-1-oleoylglycerol
-
685341
Saccharomyces cerevisiae
? + sn-1-oleoylphosphatidylcholine
-
-
-
?
sn-1,2-dioleoylphosphatidylcholine + sn-2-oleoylglycerol
-
685341
Saccharomyces cerevisiae
? + sn-1-oleoylphosphatidylcholine
-
-
-
?
sn-1,2-dioleoylphosphatidylethanolamine + sn-1,2-dioleoylglycerol
-
685341
Saccharomyces cerevisiae
trioleoylglycerol + 1-oleoylphosphatidylethanolamine
-
-
-
?
sn-1,3-dioleoylglycerol + sn-1,3-dioleoylglycerol
-
685341
Saccharomyces cerevisiae
trioleoylglycerol + sn-1-oleoylglycerol
-
-
-
?
Application (protein specific)
Application
Commentary
Organism
molecular biology
the conversion of a membrane bound lipid metabolizing enzyme into a soluble and active form might be applied to other enzymes with a membrane anchor region.
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli, expression in Pichia pastoris of a N-terminal deleted version of PDAT, lacking the predicted membrane-spanning region under the control of the methanol inducible alcohol oxidase promoter
Saccharomyces cerevisiae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
soluble form is produced for comparison with the native membrane bound enzyme
Saccharomyces cerevisiae
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
67000
-
SDS-PAGE, after deglycosylation
Saccharomyces cerevisiae
80000
-
SDS-PAGE
Saccharomyces cerevisiae
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
extensive deglycosylation severely decreases the activity
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
Commentary
Organism
the recombinant protein by Ni2+ affinity chromatography
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00138
-
sn-1,2-distearoylphosphatidylethanolamine as substrate using the membrane bound enzyme
Saccharomyces cerevisiae
0.001399
-
sn-1,2-dipalmitoylphosphatidylethanolamine as substrate using the membrane bound enzyme
Saccharomyces cerevisiae
0.002453
-
sn-1,2-dilinolenoylphosphatidylethanolamine as substrate using the membrane bound enzyme
Saccharomyces cerevisiae
0.002468
-
sn-1,2-dioleoylphosphatidylethanolamine as substrate using the membrane bound enzyme
Saccharomyces cerevisiae
0.002597
-
sn-1,2-dilinoleoylphosphatidylethanolamine as substrate using the membrane bound enzyme
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
rac-1,2-divernoleoylglycerol + 1,3-dioleoyl-sn-glycerol
-
685341
Saccharomyces cerevisiae
1-oleoyl-sn-glycerol + rac-1,2-divernoleoyl-3-oleoylglycerol
-
-
-
?
sn-1,2-dioleoylglycerol + sn-1,2-dioleoylglycerol
-
685341
Saccharomyces cerevisiae
trioleoylglycerol + sn-1-oleoylglycerol
-
-
-
?
sn-1,2-dioleoylphosphatidylcholine + sn-1,2-dioleoylglycerol
-
685341
Saccharomyces cerevisiae
trioleoylglycerol + sn-1-oleoylphosphatidylcholine
-
-
-
?
sn-1,2-dioleoylphosphatidylcholine + sn-1-oleoylglycerol
-
685341
Saccharomyces cerevisiae
? + sn-1-oleoylphosphatidylcholine
-
-
-
?
sn-1,2-dioleoylphosphatidylcholine + sn-2-oleoylglycerol
-
685341
Saccharomyces cerevisiae
? + sn-1-oleoylphosphatidylcholine
-
-
-
?
sn-1,2-dioleoylphosphatidylethanolamine + sn-1,2-dioleoylglycerol
-
685341
Saccharomyces cerevisiae
trioleoylglycerol + 1-oleoylphosphatidylethanolamine
-
-
-
?
sn-1,3-dioleoylglycerol + sn-1,3-dioleoylglycerol
-
685341
Saccharomyces cerevisiae
trioleoylglycerol + sn-1-oleoylglycerol
-
-
-
?
Other publictions for EC 2.3.1.158
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736102
Hung
Functional study of diacylglyc ...
Chlamydomonas reinhardtii
FEBS Lett.
587
2364-2370
2013
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1
3
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736422
Pan
Identification of a pair of ph ...
Linum usitatissimum
J. Biol. Chem.
288
24173-24188
2013
1
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1
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2
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5
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2
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1
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2
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4
2
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2
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1
2
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736985
Fan
Dual role for phospholipid:dia ...
Arabidopsis thaliana
Plant Cell
25
3506-3518
2013
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1
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3
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1
1
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720694
Yoon
Phospholipid:diacylglycerol ac ...
Chlamydomonas reinhardtii
Plant Cell
24
3708-3724
2012
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2
1
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-
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2
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3
1
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2
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1
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2
1
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1
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2
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1
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1
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2
1
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1
1
3
3
1
-
-
721119
Zhang
Three diacylglycerol acyltrans ...
Yarrowia lipolytica, Yarrowia lipolytica ATCC 20362
Yeast
29
25-38
2012
-
-
1
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1
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5
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1
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1
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720737
van Erp
Castor phospholipid:diacylglyc ...
Ricinus communis
Plant Physiol.
155
683-693
2011
-
1
1
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-
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1
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1
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3
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2
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1
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1
1
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1
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2
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1
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697014
Dauk
-
The role of diacylglycerol acy ...
Arabidopsis thaliana
Botany
87
552-560
2009
-
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1
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1
1
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-
684576
Arabolaza
Multiple pathways for triacylg ...
Streptomyces coelicolor
Appl. Environ. Microbiol.
74
2573-2582
2008
-
-
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-
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4
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1
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1
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2
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687725
Czabany
Structural and biochemical pro ...
Saccharomyces cerevisiae
J. Biol. Chem.
283
17065-17074
2008
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1
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1
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1
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688089
Hernandez
The utilization and desaturati ...
Olea europaea
J. Exp. Bot.
59
2425-2435
2008
-
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1
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1
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1
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1
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-
685341
Ghosal
Saccharomyces cerevisiae phosp ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1771
1457-1463
2007
-
1
1
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1
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2
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3
-
1
1
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5
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7
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1
1
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1
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2
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1
1
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5
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7
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-
676641
Mhaske
Isolation and characterization ...
Arabidopsis thaliana
Plant Physiol. Biochem.
43
413-417
2005
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1
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5
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660241
Stahl
Cloning and functional charact ...
Arabidopsis thaliana
Plant Physiol.
135
1324-1335
2004
-
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1
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1
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1
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1
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6
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1
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2
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2
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1
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1
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1
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1
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2
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2
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-
393081
Banas
The involvement of phospholipi ...
Arabidopsis thaliana, Crepis palaestina, Euphorbia lagascae, Ricinus communis
Biochem. Soc. Trans.
28
703-705
2000
-
-
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4
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9
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4
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18
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4
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4
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18
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393082
Dahlqvist
Phospholipid:diacylglycerol ac ...
Crepis palaestina, Helianthus annuus, Ricinus communis, Saccharomyces cerevisiae
Proc. Natl. Acad. Sci. USA
97
6487-6492
2000
-
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1
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4
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10
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3
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24
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1
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4
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3
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24
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