BRENDA - Enzyme Database
show all sequences of 2.3.1.158

Phospholipid:diacylglycerol acyltransferase: an enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants

Dahlqvist, A.; Stahl, U.; Lenman, M.; Banas, A.; Lee, M.; Sandager, L.; Ronne, H.; Stymne, S.; Proc. Natl. Acad. Sci. USA 97, 6487-6492 (2000)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
the gene encoding the enzyme is YNR008w, overexpression of the enzyme-encoding gene increases triacylglycerol content in yeast cells
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
phospholipid + 1,2-diacylglycerol
Saccharomyces cerevisiae
-
glycerolphosphocholine + triacylglycerol
-
Saccharomyces cerevisiae
?
phospholipid + 1,2-diacylglycerol
Ricinus communis
-
glycerolphosphocholine + triacylglycerol
-
Ricinus communis
?
phospholipid + 1,2-diacylglycerol
Helianthus annuus
-
glycerolphosphocholine + triacylglycerol
-
Helianthus annuus
?
phospholipid + 1,2-diacylglycerol
Crepis palaestina
-
glycerolphosphocholine + triacylglycerol
-
Crepis palaestina
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Crepis palaestina
-
-
-
Helianthus annuus
-
-
-
Ricinus communis
-
-
-
Saccharomyces cerevisiae
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seed
-
Crepis palaestina
-
seed
-
Helianthus annuus
-
seed
-
Ricinus communis
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-dioleoylphosphatidylcholine + 1,2-diacylglycerol
-
393082
Saccharomyces cerevisiae
1-oleoyl-2-lysophosphatidylcholine + triacylglycerol
-
393082
Saccharomyces cerevisiae
?
1,2-dioleoylphosphatidylcholine + 1,2-diacylglycerol
-
393082
Ricinus communis
1-oleoyl-2-lysophosphatidylcholine + triacylglycerol
-
393082
Ricinus communis
?
1,2-dioleoylphosphatidylcholine + 1,2-diacylglycerol
-
393082
Helianthus annuus
1-oleoyl-2-lysophosphatidylcholine + triacylglycerol
-
393082
Helianthus annuus
?
1,2-dioleoylphosphatidylcholine + 1,2-diacylglycerol
-
393082
Crepis palaestina
1-oleoyl-2-lysophosphatidylcholine + triacylglycerol
-
393082
Crepis palaestina
?
1,2-dioleoylphosphatidylethanolamine + 1,2-dioleoylglycerol
-
393082
Saccharomyces cerevisiae
1-oleoyl-2-lysophosphatidylethanolamine + trioleoylglycerol
-
393082
Saccharomyces cerevisiae
?
dioleoyl-phosphatidylcholine + 1,2-diacylglycerol
-
393082
Saccharomyces cerevisiae
lyso-phosphatidylcholine + triacylglycerol
-
393082
Saccharomyces cerevisiae
?
dioleoylphosphatidylcholine + 1,2-dioleoylglycerol
-
393082
Saccharomyces cerevisiae
1-oleoyl-2-lyso-phosphatidylcholine + trioleoylglycerol
-
393082
Saccharomyces cerevisiae
?
dioleoylphosphatidylcholine + 1-ricinoleoylglycerol
-
393082
Saccharomyces cerevisiae
1-oleoyl-2-lysophosphatidylcholine + 1-ricinoleoyl-2-oleoyl-glycerol
-
393082
Saccharomyces cerevisiae
?
dioleoylphosphatidylcholine + 1-vernoloylglycerol
-
393082
Saccharomyces cerevisiae
1-oleoyl-2-lysophosphatidylcholine + 1-vernoloyl-2-oleoylglycerol
-
393082
Saccharomyces cerevisiae
?
phospholipid + 1,2-diacylglycerol
-
393082
Saccharomyces cerevisiae
glycerolphosphocholine + triacylglycerol
-
393082
Saccharomyces cerevisiae
?
phospholipid + 1,2-diacylglycerol
-
393082
Ricinus communis
glycerolphosphocholine + triacylglycerol
-
393082
Ricinus communis
?
phospholipid + 1,2-diacylglycerol
-
393082
Helianthus annuus
glycerolphosphocholine + triacylglycerol
-
393082
Helianthus annuus
?
phospholipid + 1,2-diacylglycerol
-
393082
Crepis palaestina
glycerolphosphocholine + triacylglycerol
-
393082
Crepis palaestina
?
ricinoleoyl-phosphatidylcholine + 1,2-diacylglycerol
-
393082
Saccharomyces cerevisiae
lyso-phosphatidylcholine + triacylglycerol
-
393082
Saccharomyces cerevisiae
?
ricinoleoyl-phosphatidylcholine + 1,2-diacylglycerol
-
393082
Ricinus communis
lyso-phosphatidylcholine + triacylglycerol
-
393082
Ricinus communis
?
ricinoleoyl-phosphatidylcholine + 1,2-diacylglycerol
-
393082
Helianthus annuus
lyso-phosphatidylcholine + triacylglycerol
-
393082
Helianthus annuus
?
ricinoleoyl-phosphatidylcholine + 1,2-diacylglycerol
-
393082
Crepis palaestina
lyso-phosphatidylcholine + triacylglycerol
-
393082
Crepis palaestina
?
sn-2-acyl-phosphatidylcholine + 1,2-diacylglycerol
-
393082
Ricinus communis
triacylglycerol + glycerophosphocholine
-
393082
Ricinus communis
?
sn-2-acyl-phosphatidylcholine + 1,2-diacylglycerol
-
393082
Helianthus annuus
triacylglycerol + glycerophosphocholine
-
393082
Helianthus annuus
?
sn-2-acyl-phosphatidylcholine + 1,2-diacylglycerol
-
393082
Crepis palaestina
triacylglycerol + glycerophosphocholine
-
393082
Crepis palaestina
?
vernoloyl-phosphatidylcholine + 1,2-diacylglycerol
-
393082
Saccharomyces cerevisiae
lyso-phosphatidylcholine + triacylglycerol
-
393082
Saccharomyces cerevisiae
?
vernoloyl-phosphatidylcholine + 1,2-diacylglycerol
-
393082
Ricinus communis
lyso-phosphatidylcholine + triacylglycerol
-
393082
Ricinus communis
?
vernoloyl-phosphatidylcholine + 1,2-diacylglycerol
-
393082
Helianthus annuus
lyso-phosphatidylcholine + triacylglycerol
-
393082
Helianthus annuus
?
vernoloyl-phosphatidylcholine + 1,2-diacylglycerol
-
393082
Crepis palaestina
lyso-phosphatidylcholine + triacylglycerol
-
393082
Crepis palaestina
?
Cloned(Commentary) (protein specific)
Commentary
Organism
the gene encoding the enzyme is YNR008w, overexpression of the enzyme-encoding gene increases triacylglycerol content in yeast cells
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
phospholipid + 1,2-diacylglycerol
Saccharomyces cerevisiae
-
glycerolphosphocholine + triacylglycerol
-
Saccharomyces cerevisiae
?
phospholipid + 1,2-diacylglycerol
Ricinus communis
-
glycerolphosphocholine + triacylglycerol
-
Ricinus communis
?
phospholipid + 1,2-diacylglycerol
Helianthus annuus
-
glycerolphosphocholine + triacylglycerol
-
Helianthus annuus
?
phospholipid + 1,2-diacylglycerol
Crepis palaestina
-
glycerolphosphocholine + triacylglycerol
-
Crepis palaestina
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seed
-
Crepis palaestina
-
seed
-
Helianthus annuus
-
seed
-
Ricinus communis
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-dioleoylphosphatidylcholine + 1,2-diacylglycerol
-
393082
Saccharomyces cerevisiae
1-oleoyl-2-lysophosphatidylcholine + triacylglycerol
-
393082
Saccharomyces cerevisiae
?
1,2-dioleoylphosphatidylcholine + 1,2-diacylglycerol
-
393082
Ricinus communis
1-oleoyl-2-lysophosphatidylcholine + triacylglycerol
-
393082
Ricinus communis
?
1,2-dioleoylphosphatidylcholine + 1,2-diacylglycerol
-
393082
Helianthus annuus
1-oleoyl-2-lysophosphatidylcholine + triacylglycerol
-
393082
Helianthus annuus
?
1,2-dioleoylphosphatidylcholine + 1,2-diacylglycerol
-
393082
Crepis palaestina
1-oleoyl-2-lysophosphatidylcholine + triacylglycerol
-
393082
Crepis palaestina
?
1,2-dioleoylphosphatidylethanolamine + 1,2-dioleoylglycerol
-
393082
Saccharomyces cerevisiae
1-oleoyl-2-lysophosphatidylethanolamine + trioleoylglycerol
-
393082
Saccharomyces cerevisiae
?
dioleoyl-phosphatidylcholine + 1,2-diacylglycerol
-
393082
Saccharomyces cerevisiae
lyso-phosphatidylcholine + triacylglycerol
-
393082
Saccharomyces cerevisiae
?
dioleoylphosphatidylcholine + 1,2-dioleoylglycerol
-
393082
Saccharomyces cerevisiae
1-oleoyl-2-lyso-phosphatidylcholine + trioleoylglycerol
-
393082
Saccharomyces cerevisiae
?
dioleoylphosphatidylcholine + 1-ricinoleoylglycerol
-
393082
Saccharomyces cerevisiae
1-oleoyl-2-lysophosphatidylcholine + 1-ricinoleoyl-2-oleoyl-glycerol
-
393082
Saccharomyces cerevisiae
?
dioleoylphosphatidylcholine + 1-vernoloylglycerol
-
393082
Saccharomyces cerevisiae
1-oleoyl-2-lysophosphatidylcholine + 1-vernoloyl-2-oleoylglycerol
-
393082
Saccharomyces cerevisiae
?
phospholipid + 1,2-diacylglycerol
-
393082
Saccharomyces cerevisiae
glycerolphosphocholine + triacylglycerol
-
393082
Saccharomyces cerevisiae
?
phospholipid + 1,2-diacylglycerol
-
393082
Ricinus communis
glycerolphosphocholine + triacylglycerol
-
393082
Ricinus communis
?
phospholipid + 1,2-diacylglycerol
-
393082
Helianthus annuus
glycerolphosphocholine + triacylglycerol
-
393082
Helianthus annuus
?
phospholipid + 1,2-diacylglycerol
-
393082
Crepis palaestina
glycerolphosphocholine + triacylglycerol
-
393082
Crepis palaestina
?
ricinoleoyl-phosphatidylcholine + 1,2-diacylglycerol
-
393082
Saccharomyces cerevisiae
lyso-phosphatidylcholine + triacylglycerol
-
393082
Saccharomyces cerevisiae
?
ricinoleoyl-phosphatidylcholine + 1,2-diacylglycerol
-
393082
Ricinus communis
lyso-phosphatidylcholine + triacylglycerol
-
393082
Ricinus communis
?
ricinoleoyl-phosphatidylcholine + 1,2-diacylglycerol
-
393082
Helianthus annuus
lyso-phosphatidylcholine + triacylglycerol
-
393082
Helianthus annuus
?
ricinoleoyl-phosphatidylcholine + 1,2-diacylglycerol
-
393082
Crepis palaestina
lyso-phosphatidylcholine + triacylglycerol
-
393082
Crepis palaestina
?
sn-2-acyl-phosphatidylcholine + 1,2-diacylglycerol
-
393082
Ricinus communis
triacylglycerol + glycerophosphocholine
-
393082
Ricinus communis
?
sn-2-acyl-phosphatidylcholine + 1,2-diacylglycerol
-
393082
Helianthus annuus
triacylglycerol + glycerophosphocholine
-
393082
Helianthus annuus
?
sn-2-acyl-phosphatidylcholine + 1,2-diacylglycerol
-
393082
Crepis palaestina
triacylglycerol + glycerophosphocholine
-
393082
Crepis palaestina
?
vernoloyl-phosphatidylcholine + 1,2-diacylglycerol
-
393082
Saccharomyces cerevisiae
lyso-phosphatidylcholine + triacylglycerol
-
393082
Saccharomyces cerevisiae
?
vernoloyl-phosphatidylcholine + 1,2-diacylglycerol
-
393082
Ricinus communis
lyso-phosphatidylcholine + triacylglycerol
-
393082
Ricinus communis
?
vernoloyl-phosphatidylcholine + 1,2-diacylglycerol
-
393082
Helianthus annuus
lyso-phosphatidylcholine + triacylglycerol
-
393082
Helianthus annuus
?
vernoloyl-phosphatidylcholine + 1,2-diacylglycerol
-
393082
Crepis palaestina
lyso-phosphatidylcholine + triacylglycerol
-
393082
Crepis palaestina
?
Other publictions for EC 2.3.1.158
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736102
Hung
Functional study of diacylglyc ...
Chlamydomonas reinhardtii
FEBS Lett.
587
2364-2370
2013
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1
3
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736422
Pan
Identification of a pair of ph ...
Linum usitatissimum
J. Biol. Chem.
288
24173-24188
2013
1
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1
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1
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4
2
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2
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1
2
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736985
Fan
Dual role for phospholipid:dia ...
Arabidopsis thaliana
Plant Cell
25
3506-3518
2013
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1
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3
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1
1
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720694
Yoon
Phospholipid:diacylglycerol ac ...
Chlamydomonas reinhardtii
Plant Cell
24
3708-3724
2012
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2
1
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2
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3
1
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2
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1
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1
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2
1
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1
1
3
3
1
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721119
Zhang
Three diacylglycerol acyltrans ...
Yarrowia lipolytica, Yarrowia lipolytica ATCC 20362
Yeast
29
25-38
2012
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1
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1
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5
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1
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1
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720737
van Erp
Castor phospholipid:diacylglyc ...
Ricinus communis
Plant Physiol.
155
683-693
2011
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1
1
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1
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3
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2
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1
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697014
Dauk
-
The role of diacylglycerol acy ...
Arabidopsis thaliana
Botany
87
552-560
2009
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1
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1
1
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684576
Arabolaza
Multiple pathways for triacylg ...
Streptomyces coelicolor
Appl. Environ. Microbiol.
74
2573-2582
2008
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4
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687725
Czabany
Structural and biochemical pro ...
Saccharomyces cerevisiae
J. Biol. Chem.
283
17065-17074
2008
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1
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688089
Hernandez
The utilization and desaturati ...
Olea europaea
J. Exp. Bot.
59
2425-2435
2008
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685341
Ghosal
Saccharomyces cerevisiae phosp ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1771
1457-1463
2007
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1
1
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1
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1
1
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7
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1
1
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1
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1
1
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5
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7
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676641
Mhaske
Isolation and characterization ...
Arabidopsis thaliana
Plant Physiol. Biochem.
43
413-417
2005
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1
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5
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1
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6
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2
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2
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1
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1
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1
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2
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2
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18
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4
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18
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24
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1
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24
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