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Literature summary for 2.3.1.157 extracted from

  • Wang, M.; Huang, M.; Gu, H.; Li, S.; Ma, Y.; Wang, J.
    Mutational analysis to identify the residues essential for the acetyltransferase activity of GlmU in Bacillus subtilis (2017), RSC Adv., 7, 13858-13867 .
No PubMed abstract available
Search for more literature for 2.3.1.157

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli
expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
C380A the acetyltransferase activity of the mutant is 2.6fold higher than that of the wild type enzyme Bacillus subtilis
C380A/C405S the double mutant has an increased kcat/Km value compared to the wild type enzyme Bacillus subtilis
C405A the mutant shows a slight increase in activity compared to the wild type enzyme Bacillus subtilis
C405S the mutant has an increased kcat/Km value compared to the wild type enzyme Bacillus subtilis
V385C/C405S the double mutant has an increased kcat/Km value compared to the wild type enzyme Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.199
-
 acetyl-CoA mutant enzyme V385C/C405S, at pH 8.0 and 37°C Bacillus subtilis
0.241
-
 alpha-D-glucosamine 1-phosphate mutant enzyme C380A, at pH 8.0 and 37°C Bacillus subtilis
0.278
-
 alpha-D-glucosamine 1-phosphate mutant enzyme C405A, at pH 8.0 and 37°C Bacillus subtilis
0.304
-
 alpha-D-glucosamine 1-phosphate mutant enzyme V385C/C405S, at pH 8.0 and 37°C Bacillus subtilis
0.333
-
 alpha-D-glucosamine 1-phosphate wild type enzyme, at pH 8.0 and 37°C Bacillus subtilis
0.359
-
 alpha-D-glucosamine 1-phosphate mutant enzyme C405S, at pH 8.0 and 37°C Bacillus subtilis
0.47
-
 alpha-D-glucosamine 1-phosphate mutant enzyme C380A/C405S, at pH 8.0 and 37°C Bacillus subtilis
0.603
-
 acetyl-CoA mutant enzyme C380A, at pH 8.0 and 37°C Bacillus subtilis
0.638
-
 acetyl-CoA mutant enzyme C380A/C405S, at pH 8.0 and 37°C Bacillus subtilis
1.213
-
 acetyl-CoA mutant enzyme C405A, at pH 8.0 and 37°C Bacillus subtilis
1.821
-
 acetyl-CoA wild type enzyme, at pH 8.0 and 37°C Bacillus subtilis
1.963
-
 acetyl-CoA mutant enzyme C405S, at pH 8.0 and 37°C Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the concentration of Mg2+ has no effect on the enzymatic activity Escherichia coli
Mg2+ the enzyme activity is optimal at 15 mM Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + alpha-D-glucosamine 1-phosphate Escherichia coli
-
 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
acetyl-CoA + alpha-D-glucosamine 1-phosphate Bacillus subtilis
-
 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
acetyl-CoA + alpha-D-glucosamine 1-phosphate Bacillus subtilis 168
-
 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P14192
-
-
Bacillus subtilis 168 P14192
-
-
Escherichia coli P0ACC7
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA His-binding column chromatography Escherichia coli
Ni-NTA His-binding column chromatography Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
 Escherichia coli CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
 Bacillus subtilis CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
 Bacillus subtilis 168 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 52000, SDS-PAGE Escherichia coli
? x * 52000, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
GlmU bifunctional enzyme with glucosamine 1-phosphate acetyltransferase and N-acetylglucosamine 1-phosphate uridyltransferase activities Escherichia coli
GlmU bifunctional enzyme with glucosamine 1-phosphate acetyltransferase and N-acetylglucosamine 1-phosphate uridyltransferase activities Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Escherichia coli
37
-
-
 Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.81
-
 alpha-D-glucosamine 1-phosphate wild type enzyme, at pH 8.0 and 37°C Bacillus subtilis
2.407
-
 alpha-D-glucosamine 1-phosphate mutant enzyme C405S, at pH 8.0 and 37°C Bacillus subtilis
2.459
-
 acetyl-CoA wild type enzyme, at pH 8.0 and 37°C Bacillus subtilis
3.112
-
 alpha-D-glucosamine 1-phosphate mutant enzyme C405A, at pH 8.0 and 37°C Bacillus subtilis
5.496
-
 acetyl-CoA mutant enzyme C405A, at pH 8.0 and 37°C Bacillus subtilis
6.629
-
 acetyl-CoA mutant enzyme V385C/C405S, at pH 8.0 and 37°C Bacillus subtilis
15.756
-
 alpha-D-glucosamine 1-phosphate mutant enzyme V385C/C405S, at pH 8.0 and 37°C Bacillus subtilis
18.512
-
 acetyl-CoA mutant enzyme C405S, at pH 8.0 and 37°C Bacillus subtilis
25.441
-
 alpha-D-glucosamine 1-phosphate mutant enzyme C380A, at pH 8.0 and 37°C Bacillus subtilis
26.147
-
 acetyl-CoA mutant enzyme C380A/C405S, at pH 8.0 and 37°C Bacillus subtilis
30.783
-
 alpha-D-glucosamine 1-phosphate mutant enzyme C380A/C405S, at pH 8.0 and 37°C Bacillus subtilis
54.617
-
 acetyl-CoA mutant enzyme C380A, at pH 8.0 and 37°C Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Escherichia coli
8
-
-
 Bacillus subtilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.35
-
 acetyl-CoA wild type enzyme, at pH 8.0 and 37°C Bacillus subtilis
2.432
-
 alpha-D-glucosamine 1-phosphate wild type enzyme, at pH 8.0 and 37°C Bacillus subtilis
4.531
-
 acetyl-CoA mutant enzyme C405A, at pH 8.0 and 37°C Bacillus subtilis
6.705
-
 alpha-D-glucosamine 1-phosphate mutant enzyme C405S, at pH 8.0 and 37°C Bacillus subtilis
9.43
-
 acetyl-CoA mutant enzyme C405S, at pH 8.0 and 37°C Bacillus subtilis
11.194
-
 alpha-D-glucosamine 1-phosphate mutant enzyme C405A, at pH 8.0 and 37°C Bacillus subtilis
33.262
-
 acetyl-CoA mutant enzyme V385C/C405S, at pH 8.0 and 37°C Bacillus subtilis
40.977
-
 acetyl-CoA mutant enzyme C380A/C405S, at pH 8.0 and 37°C Bacillus subtilis
51.829
-
 alpha-D-glucosamine 1-phosphate mutant enzyme V385C/C405S, at pH 8.0 and 37°C Bacillus subtilis
65.496
-
 alpha-D-glucosamine 1-phosphate mutant enzyme C380A/C405S, at pH 8.0 and 37°C Bacillus subtilis
90.546
-
 acetyl-CoA mutant enzyme C380A, at pH 8.0 and 37°C Bacillus subtilis
105.564
-
 alpha-D-glucosamine 1-phosphate mutant enzyme C380A, at pH 8.0 and 37°C Bacillus subtilis