BRENDA - Enzyme Database
show all sequences of 2.3.1.157

In vitro validation of acetyltransferase activity of GlmU as an antibacterial target in Haemophilus influenzae

Buurman, E.T.; Andrews, B.; Gao, N.; Hu, J.; Keating, T.A.; Lahiri, S.; Otterbein, L.R.; Patten, A.D.; Stokes, S.S.; Shapiro, A.B.; J. Biol. Chem. 286, 40734-40742 (2011)

Data extracted from this reference:

Application
Application
Commentary
Organism
drug development
GlmU is a target for inhibitor design
Escherichia coli
drug development
GlmU is a target for inhibitor design
Haemophilus influenzae
drug development
GlmU is a target for inhibitor design
Staphylococcus aureus
drug development
GlmU is a target for inhibitor design
Streptococcus pneumoniae
Cloned(Commentary)
Commentary
Organism
gene glmU, expression in Escherichia coli strain BL21(DE3)
Haemophilus influenzae
gene glmU, expression in Escherichia coli strain BL21(DE3)
Staphylococcus aureus
gene glmU, expression in Escherichia coli strain HMS174(DE3)
Escherichia coli
gene glmU, expression in Escherichia coli strain HMS174(DE3)
Streptococcus pneumoniae
Inhibitors
Inhibitors
Commentary
Organism
Structure
4-([4-hydroxy-2-methoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]amino)-4-oxobutanoic acid
-
Escherichia coli
4-([4-hydroxy-2-methoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]amino)-4-oxobutanoic acid
-
Haemophilus influenzae
4-([4-hydroxy-2-methoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]amino)-4-oxobutanoic acid
-
Staphylococcus aureus
4-([4-hydroxy-2-methoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]amino)-4-oxobutanoic acid
-
Streptococcus pneumoniae
4-([5-[(4-aminophenyl)(phenyl)sulfamoyl]-2,4-dimethoxyphenyl]amino)-4-oxobutanoic acid
-
Escherichia coli
4-([5-[(4-aminophenyl)(phenyl)sulfamoyl]-2,4-dimethoxyphenyl]amino)-4-oxobutanoic acid
-
Haemophilus influenzae
4-([5-[(4-aminophenyl)(phenyl)sulfamoyl]-2,4-dimethoxyphenyl]amino)-4-oxobutanoic acid
-
Staphylococcus aureus
4-([5-[(4-aminophenyl)(phenyl)sulfamoyl]-2,4-dimethoxyphenyl]amino)-4-oxobutanoic acid
-
Streptococcus pneumoniae
4-[[2,4-dimethoxy-5-(10H-phenoxazin-10-ylsulfonyl)phenyl]amino]-4-oxobutanoic acid
substrate inhibition, competitive versus N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
Haemophilus influenzae
4-[[2,4-dimethoxy-5-(10H-phenoxazin-10-ylsulfonyl)phenyl]amino]-4-oxobutanoic acid
-
Staphylococcus aureus
4-[[2,4-dimethoxy-5-(10H-phenoxazin-10-ylsulfonyl)phenyl]amino]-4-oxobutanoic acid
-
Streptococcus pneumoniae
4-[[2,4-dimethoxy-5-(10H-phenoxazin-10-ylsulfonyl)phenyl]amino]-4-oxobutanoic acid
-
Escherichia coli
alpha-D-glucosamine 1-phosphate
substrate inhibition, competitive versus N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
Haemophilus influenzae
additional information
inhibitor design from enzyme structure, analysis of antimicrobial compounds mediating their growth inhibitory effects specifically via GlmU, antimicrobial properties of sulfonamide inhibitors of GlmU acetyl transferase, overview
Escherichia coli
additional information
inhibitor design from enzyme structure, analysis of antimicrobial compounds mediating their growth inhibitory effects specifically via GlmU, antimicrobial properties of sulfonamide inhibitors of GlmU acetyl transferase, overview
Haemophilus influenzae
additional information
inhibitor design from enzyme structure, analysis of antimicrobial compounds mediating their growth inhibitory effects specifically via GlmU, antimicrobial properties of sulfonamide inhibitors of GlmU acetyl transferase, overview
Staphylococcus aureus
additional information
inhibitor design from enzyme structure, analysis of antimicrobial compounds mediating their growth inhibitory effects specifically via GlmU, antimicrobial properties of sulfonamide inhibitors of GlmU acetyl transferase, overview
Streptococcus pneumoniae
N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
-
Escherichia coli
N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
-
Haemophilus influenzae
N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
-
Staphylococcus aureus
N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
-
Streptococcus pneumoniae
N-[2,4-dimethoxy-5-[methyl(phenyl)sulfamoyl]phenyl]acetamide
-
Escherichia coli
N-[2,4-dimethoxy-5-[methyl(phenyl)sulfamoyl]phenyl]acetamide
-
Haemophilus influenzae
N-[2,4-dimethoxy-5-[methyl(phenyl)sulfamoyl]phenyl]acetamide
-
Staphylococcus aureus
N-[2,4-dimethoxy-5-[methyl(phenyl)sulfamoyl]phenyl]acetamide
-
Streptococcus pneumoniae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + alpha-D-glucosamine 1-phosphate
Staphylococcus aureus
-
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
Haemophilus influenzae
-
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
Escherichia coli
-
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
Streptococcus pneumoniae
-
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
Streptococcus pneumoniae NCTC 7466
-
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
Escherichia coli ATCC 27325
-
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
Haemophilus influenzae ATCC 51907
-
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
Staphylococcus aureus RN4220
-
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Escherichia coli ATCC 27325
-
-
-
Haemophilus influenzae
-
-
-
Haemophilus influenzae ATCC 51907
-
-
-
Staphylococcus aureus
-
-
-
Staphylococcus aureus RN4220
-
-
-
Streptococcus pneumoniae
-
-
-
Streptococcus pneumoniae NCTC 7466
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant GlmU from Escherichia coli strain BL21(DE3) by anion exchange and hydrophobic interaction chromatography and gel filtration
Haemophilus influenzae
recombinant GlmU from Escherichia coli strain BL21(DE3) by anion exchange and hydrophobic interaction chromatography and gel filtration
Staphylococcus aureus
recombinant GlmU from Escherichia coli strain HMS174(DE3) by anion exchange and hydrophobic interaction chromatography and gel filtration
Escherichia coli
recombinant GlmU from Escherichia coli strain HMS174(DE3) by anion exchange and hydrophobic interaction chromatography and gel filtration
Streptococcus pneumoniae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
719986
Staphylococcus aureus
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
719986
Haemophilus influenzae
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
719986
Escherichia coli
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
719986
Streptococcus pneumoniae
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
719986
Streptococcus pneumoniae NCTC 7466
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
719986
Escherichia coli ATCC 27325
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
719986
Haemophilus influenzae ATCC 51907
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
719986
Staphylococcus aureus RN4220
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
Escherichia coli
7.2
-
assay at
Haemophilus influenzae
7.2
-
assay at
Staphylococcus aureus
7.2
-
assay at
Streptococcus pneumoniae
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
additional information
-
additional information
inhibition kinetics, ping pong bisubstrate rate, overview
Haemophilus influenzae
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.000007
-
pH 7.2, temperature not specified in the publication
Escherichia coli
4-[[2,4-dimethoxy-5-(10H-phenoxazin-10-ylsulfonyl)phenyl]amino]-4-oxobutanoic acid
0.000018
-
pH 7.2, temperature not specified in the publication
Escherichia coli
4-([5-[(4-aminophenyl)(phenyl)sulfamoyl]-2,4-dimethoxyphenyl]amino)-4-oxobutanoic acid
0.000023
-
pH 7.2, temperature not specified in the publication
Haemophilus influenzae
4-[[2,4-dimethoxy-5-(10H-phenoxazin-10-ylsulfonyl)phenyl]amino]-4-oxobutanoic acid
0.000058
-
pH 7.2, temperature not specified in the publication
Escherichia coli
4-([4-hydroxy-2-methoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]amino)-4-oxobutanoic acid
0.00028
-
pH 7.2, temperature not specified in the publication
Haemophilus influenzae
4-([4-hydroxy-2-methoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]amino)-4-oxobutanoic acid
0.0004
-
pH 7.2, temperature not specified in the publication
Haemophilus influenzae
4-([5-[(4-aminophenyl)(phenyl)sulfamoyl]-2,4-dimethoxyphenyl]amino)-4-oxobutanoic acid
0.0021
-
pH 7.2, temperature not specified in the publication
Escherichia coli
N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
0.0033
-
pH 7.2, temperature not specified in the publication
Escherichia coli
N-[2,4-dimethoxy-5-[methyl(phenyl)sulfamoyl]phenyl]acetamide
0.0047
-
pH 7.2, temperature not specified in the publication
Streptococcus pneumoniae
4-([4-hydroxy-2-methoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]amino)-4-oxobutanoic acid
0.0064
-
pH 7.2, temperature not specified in the publication
Haemophilus influenzae
N-[2,4-dimethoxy-5-[methyl(phenyl)sulfamoyl]phenyl]acetamide
0.0078
-
pH 7.2, temperature not specified in the publication
Haemophilus influenzae
N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
0.015
-
pH 7.2, temperature not specified in the publication
Streptococcus pneumoniae
4-[[2,4-dimethoxy-5-(10H-phenoxazin-10-ylsulfonyl)phenyl]amino]-4-oxobutanoic acid
0.04
-
pH 7.2, temperature not specified in the publication
Streptococcus pneumoniae
4-([5-[(4-aminophenyl)(phenyl)sulfamoyl]-2,4-dimethoxyphenyl]amino)-4-oxobutanoic acid
0.2
-
above, pH 7.2, temperature not specified in the publication
Staphylococcus aureus
4-[[2,4-dimethoxy-5-(10H-phenoxazin-10-ylsulfonyl)phenyl]amino]-4-oxobutanoic acid
0.2
-
above, pH 7.2, temperature not specified in the publication
Staphylococcus aureus
4-([4-hydroxy-2-methoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]amino)-4-oxobutanoic acid
0.2
-
above, pH 7.2, temperature not specified in the publication
Staphylococcus aureus
4-([5-[(4-aminophenyl)(phenyl)sulfamoyl]-2,4-dimethoxyphenyl]amino)-4-oxobutanoic acid
0.2
-
above, pH 7.2, temperature not specified in the publication
Staphylococcus aureus
N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
0.2
-
above, pH 7.2, temperature not specified in the publication
Streptococcus pneumoniae
N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
0.2
-
above, pH 7.2, temperature not specified in the publication
Staphylococcus aureus
N-[2,4-dimethoxy-5-[methyl(phenyl)sulfamoyl]phenyl]acetamide
0.2
-
above, pH 7.2, temperature not specified in the publication
Streptococcus pneumoniae
N-[2,4-dimethoxy-5-[methyl(phenyl)sulfamoyl]phenyl]acetamide
Application (protein specific)
Application
Commentary
Organism
drug development
GlmU is a target for inhibitor design
Escherichia coli
drug development
GlmU is a target for inhibitor design
Haemophilus influenzae
drug development
GlmU is a target for inhibitor design
Staphylococcus aureus
drug development
GlmU is a target for inhibitor design
Streptococcus pneumoniae
Cloned(Commentary) (protein specific)
Commentary
Organism
gene glmU, expression in Escherichia coli strain BL21(DE3)
Haemophilus influenzae
gene glmU, expression in Escherichia coli strain BL21(DE3)
Staphylococcus aureus
gene glmU, expression in Escherichia coli strain HMS174(DE3)
Escherichia coli
gene glmU, expression in Escherichia coli strain HMS174(DE3)
Streptococcus pneumoniae
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.000007
-
pH 7.2, temperature not specified in the publication
Escherichia coli
4-[[2,4-dimethoxy-5-(10H-phenoxazin-10-ylsulfonyl)phenyl]amino]-4-oxobutanoic acid
0.000018
-
pH 7.2, temperature not specified in the publication
Escherichia coli
4-([5-[(4-aminophenyl)(phenyl)sulfamoyl]-2,4-dimethoxyphenyl]amino)-4-oxobutanoic acid
0.000023
-
pH 7.2, temperature not specified in the publication
Haemophilus influenzae
4-[[2,4-dimethoxy-5-(10H-phenoxazin-10-ylsulfonyl)phenyl]amino]-4-oxobutanoic acid
0.000058
-
pH 7.2, temperature not specified in the publication
Escherichia coli
4-([4-hydroxy-2-methoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]amino)-4-oxobutanoic acid
0.00028
-
pH 7.2, temperature not specified in the publication
Haemophilus influenzae
4-([4-hydroxy-2-methoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]amino)-4-oxobutanoic acid
0.0004
-
pH 7.2, temperature not specified in the publication
Haemophilus influenzae
4-([5-[(4-aminophenyl)(phenyl)sulfamoyl]-2,4-dimethoxyphenyl]amino)-4-oxobutanoic acid
0.0021
-
pH 7.2, temperature not specified in the publication
Escherichia coli
N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
0.0033
-
pH 7.2, temperature not specified in the publication
Escherichia coli
N-[2,4-dimethoxy-5-[methyl(phenyl)sulfamoyl]phenyl]acetamide
0.0047
-
pH 7.2, temperature not specified in the publication
Streptococcus pneumoniae
4-([4-hydroxy-2-methoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]amino)-4-oxobutanoic acid
0.0064
-
pH 7.2, temperature not specified in the publication
Haemophilus influenzae
N-[2,4-dimethoxy-5-[methyl(phenyl)sulfamoyl]phenyl]acetamide
0.0078
-
pH 7.2, temperature not specified in the publication
Haemophilus influenzae
N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
0.015
-
pH 7.2, temperature not specified in the publication
Streptococcus pneumoniae
4-[[2,4-dimethoxy-5-(10H-phenoxazin-10-ylsulfonyl)phenyl]amino]-4-oxobutanoic acid
0.04
-
pH 7.2, temperature not specified in the publication
Streptococcus pneumoniae
4-([5-[(4-aminophenyl)(phenyl)sulfamoyl]-2,4-dimethoxyphenyl]amino)-4-oxobutanoic acid
0.2
-
above, pH 7.2, temperature not specified in the publication
Staphylococcus aureus
4-[[2,4-dimethoxy-5-(10H-phenoxazin-10-ylsulfonyl)phenyl]amino]-4-oxobutanoic acid
0.2
-
above, pH 7.2, temperature not specified in the publication
Staphylococcus aureus
4-([4-hydroxy-2-methoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]amino)-4-oxobutanoic acid
0.2
-
above, pH 7.2, temperature not specified in the publication
Staphylococcus aureus
4-([5-[(4-aminophenyl)(phenyl)sulfamoyl]-2,4-dimethoxyphenyl]amino)-4-oxobutanoic acid
0.2
-
above, pH 7.2, temperature not specified in the publication
Staphylococcus aureus
N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
0.2
-
above, pH 7.2, temperature not specified in the publication
Streptococcus pneumoniae
N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
0.2
-
above, pH 7.2, temperature not specified in the publication
Staphylococcus aureus
N-[2,4-dimethoxy-5-[methyl(phenyl)sulfamoyl]phenyl]acetamide
0.2
-
above, pH 7.2, temperature not specified in the publication
Streptococcus pneumoniae
N-[2,4-dimethoxy-5-[methyl(phenyl)sulfamoyl]phenyl]acetamide
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
4-([4-hydroxy-2-methoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]amino)-4-oxobutanoic acid
-
Escherichia coli
4-([4-hydroxy-2-methoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]amino)-4-oxobutanoic acid
-
Haemophilus influenzae
4-([4-hydroxy-2-methoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]amino)-4-oxobutanoic acid
-
Staphylococcus aureus
4-([4-hydroxy-2-methoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]amino)-4-oxobutanoic acid
-
Streptococcus pneumoniae
4-([5-[(4-aminophenyl)(phenyl)sulfamoyl]-2,4-dimethoxyphenyl]amino)-4-oxobutanoic acid
-
Escherichia coli
4-([5-[(4-aminophenyl)(phenyl)sulfamoyl]-2,4-dimethoxyphenyl]amino)-4-oxobutanoic acid
-
Haemophilus influenzae
4-([5-[(4-aminophenyl)(phenyl)sulfamoyl]-2,4-dimethoxyphenyl]amino)-4-oxobutanoic acid
-
Staphylococcus aureus
4-([5-[(4-aminophenyl)(phenyl)sulfamoyl]-2,4-dimethoxyphenyl]amino)-4-oxobutanoic acid
-
Streptococcus pneumoniae
4-[[2,4-dimethoxy-5-(10H-phenoxazin-10-ylsulfonyl)phenyl]amino]-4-oxobutanoic acid
-
Escherichia coli
4-[[2,4-dimethoxy-5-(10H-phenoxazin-10-ylsulfonyl)phenyl]amino]-4-oxobutanoic acid
substrate inhibition, competitive versus N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
Haemophilus influenzae
4-[[2,4-dimethoxy-5-(10H-phenoxazin-10-ylsulfonyl)phenyl]amino]-4-oxobutanoic acid
-
Staphylococcus aureus
4-[[2,4-dimethoxy-5-(10H-phenoxazin-10-ylsulfonyl)phenyl]amino]-4-oxobutanoic acid
-
Streptococcus pneumoniae
alpha-D-glucosamine 1-phosphate
substrate inhibition, competitive versus N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
Haemophilus influenzae
additional information
inhibitor design from enzyme structure, analysis of antimicrobial compounds mediating their growth inhibitory effects specifically via GlmU, antimicrobial properties of sulfonamide inhibitors of GlmU acetyl transferase, overview
Escherichia coli
additional information
inhibitor design from enzyme structure, analysis of antimicrobial compounds mediating their growth inhibitory effects specifically via GlmU, antimicrobial properties of sulfonamide inhibitors of GlmU acetyl transferase, overview
Haemophilus influenzae
additional information
inhibitor design from enzyme structure, analysis of antimicrobial compounds mediating their growth inhibitory effects specifically via GlmU, antimicrobial properties of sulfonamide inhibitors of GlmU acetyl transferase, overview
Staphylococcus aureus
additional information
inhibitor design from enzyme structure, analysis of antimicrobial compounds mediating their growth inhibitory effects specifically via GlmU, antimicrobial properties of sulfonamide inhibitors of GlmU acetyl transferase, overview
Streptococcus pneumoniae
N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
-
Escherichia coli
N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
-
Haemophilus influenzae
N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
-
Staphylococcus aureus
N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
-
Streptococcus pneumoniae
N-[2,4-dimethoxy-5-[methyl(phenyl)sulfamoyl]phenyl]acetamide
-
Escherichia coli
N-[2,4-dimethoxy-5-[methyl(phenyl)sulfamoyl]phenyl]acetamide
-
Haemophilus influenzae
N-[2,4-dimethoxy-5-[methyl(phenyl)sulfamoyl]phenyl]acetamide
-
Staphylococcus aureus
N-[2,4-dimethoxy-5-[methyl(phenyl)sulfamoyl]phenyl]acetamide
-
Streptococcus pneumoniae
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
additional information
-
additional information
inhibition kinetics, ping pong bisubstrate rate, overview
Haemophilus influenzae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + alpha-D-glucosamine 1-phosphate
Staphylococcus aureus
-
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
Haemophilus influenzae
-
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
Escherichia coli
-
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
Streptococcus pneumoniae
-
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
Streptococcus pneumoniae NCTC 7466
-
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
Escherichia coli ATCC 27325
-
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
Haemophilus influenzae ATCC 51907
-
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
Staphylococcus aureus RN4220
-
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant GlmU from Escherichia coli strain BL21(DE3) by anion exchange and hydrophobic interaction chromatography and gel filtration
Haemophilus influenzae
recombinant GlmU from Escherichia coli strain BL21(DE3) by anion exchange and hydrophobic interaction chromatography and gel filtration
Staphylococcus aureus
recombinant GlmU from Escherichia coli strain HMS174(DE3) by anion exchange and hydrophobic interaction chromatography and gel filtration
Escherichia coli
recombinant GlmU from Escherichia coli strain HMS174(DE3) by anion exchange and hydrophobic interaction chromatography and gel filtration
Streptococcus pneumoniae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
719986
Staphylococcus aureus
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
719986
Haemophilus influenzae
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
719986
Escherichia coli
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
719986
Streptococcus pneumoniae
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
719986
Streptococcus pneumoniae NCTC 7466
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
719986
Escherichia coli ATCC 27325
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
719986
Haemophilus influenzae ATCC 51907
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
719986
Staphylococcus aureus RN4220
CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
Escherichia coli
7.2
-
assay at
Haemophilus influenzae
7.2
-
assay at
Staphylococcus aureus
7.2
-
assay at
Streptococcus pneumoniae
General Information
General Information
Commentary
Organism
physiological function
GlmU is a bifunctional enzyme that is essential for bacterial growth, converting D-glucosamine 1-phosphate into UDPGlcNAc via acetylation and subsequent uridyl transfer
Escherichia coli
physiological function
GlmU is a bifunctional enzyme that is essential for bacterial growth, converting D-glucosamine 1-phosphate into UDPGlcNAc via acetylation and subsequent uridyl transfer
Haemophilus influenzae
physiological function
GlmU is a bifunctional enzyme that is essential for bacterial growth, converting D-glucosamine 1-phosphate into UDPGlcNAc via acetylation and subsequent uridyl transfer
Staphylococcus aureus
physiological function
GlmU is a bifunctional enzyme that is essential for bacterial growth, converting D-glucosamine 1-phosphate into UDPGlcNAc via acetylation and subsequent uridyl transfer
Streptococcus pneumoniae
General Information (protein specific)
General Information
Commentary
Organism
physiological function
GlmU is a bifunctional enzyme that is essential for bacterial growth, converting D-glucosamine 1-phosphate into UDPGlcNAc via acetylation and subsequent uridyl transfer
Escherichia coli
physiological function
GlmU is a bifunctional enzyme that is essential for bacterial growth, converting D-glucosamine 1-phosphate into UDPGlcNAc via acetylation and subsequent uridyl transfer
Haemophilus influenzae
physiological function
GlmU is a bifunctional enzyme that is essential for bacterial growth, converting D-glucosamine 1-phosphate into UDPGlcNAc via acetylation and subsequent uridyl transfer
Staphylococcus aureus
physiological function
GlmU is a bifunctional enzyme that is essential for bacterial growth, converting D-glucosamine 1-phosphate into UDPGlcNAc via acetylation and subsequent uridyl transfer
Streptococcus pneumoniae
Other publictions for EC 2.3.1.157
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746844
Honda
Improvement of ST0452 N-acety ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii DSM 16993
Appl. Environ. Microbiol.
84
e002213-18
2018
-
-
1
1
2
-
-
-
-
-
-
-
-
10
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
736517
Sharma
Escherichia coli N-acetylgluco ...
Escherichia coli, Escherichia coli ATCC 25922
J. Biomol. Screen.
21
342-353
2016
-
-
1
-
-
-
3
-
-
1
-
2
-
10
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
4
-
2
-
-
1
1
-
-
-
2
4
4
-
-
1
-
2
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
737096
Dziadek
Mycobacterium tuberculosis Ats ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis ATCC 25618, Mycobacterium tuberculosis H37Rv
PLoS ONE
11
e0148030
2016
-
1
1
-
1
-
-
-
-
-
-
3
-
15
-
-
1
-
-
-
-
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
729696
Zhang
Characterization of the amino ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7, Sulfurisphaera tokodaii DSM 16993 / JCM 10545 / NBRC 100140 / 7
Extremophiles
19
417-427
2015
-
1
1
-
7
-
1
9
-
1
-
6
-
8
-
-
1
-
-
-
12
-
9
2
1
-
1
6
1
-
-
1
-
-
-
-
1
1
1
-
7
-
-
1
-
9
-
1
-
6
-
-
-
1
-
-
12
-
9
2
1
-
1
6
1
-
-
-
-
2
2
-
6
6
735518
Sharma
Identification and characteriz ...
Escherichia coli, Escherichia coli ATCC 25922, Haemophilus influenzae, Haemophilus influenzae ATCC 51907
Appl. Microbiol. Biotechnol.
100
3071-3085
2015
-
-
1
-
-
-
15
-
-
-
-
8
-
12
-
-
1
-
-
-
-
-
10
-
2
-
-
-
2
-
-
2
2
-
11
-
-
1
2
-
-
-
12
17
2
-
-
-
-
8
-
-
-
1
-
-
-
-
10
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
735569
Patin
Purification and biochemical c ...
Yersinia pestis, Yersinia pestis YPIII, Yersinia pseudotuberculosis, Yersinia pseudotuberculosis YPIII
Arch. Microbiol.
197
371-378
2015
1
-
2
-
1
-
2
4
-
2
-
2
-
6
-
-
1
-
-
-
-
-
4
2
1
-
-
4
1
-
-
1
-
-
-
1
-
2
1
-
1
-
-
2
-
4
-
2
-
2
-
-
-
1
-
-
-
-
4
2
1
-
-
4
1
-
-
-
-
2
2
-
-
-
737150
Soni
Depletion of M. tuberculosis G ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis ATCC 25618, Mycobacterium tuberculosis H37Rv
PLoS Pathog.
11
e1005235
2015
-
1
1
1
-
-
2
-
-
-
-
6
-
12
-
-
-
-
-
-
-
-
6
-
1
-
-
-
1
-
-
1
-
-
1
-
1
1
1
1
-
-
1
2
-
-
-
-
-
6
-
-
-
-
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
737291
Rani
High-throughput screen identif ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Tuberculosis
95
664-677
2015
-
-
1
-
-
-
6
3
-
1
-
4
-
11
-
-
1
-
-
-
-
-
5
-
1
-
-
-
1
-
-
1
5
-
10
-
-
1
1
-
-
-
10
8
5
5
-
1
-
4
-
-
-
1
-
-
-
-
5
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
735396
Vithani
GlmU (N-acetylglucosamine-1-ph ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis ATCC 25618
Acta Crystallogr. Sect. F
70
703-708
2014
-
-
1
1
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
736058
Mehra
Identification and optimizatio ...
Escherichia coli, Escherichia coli ATCC 25922
Eur. J. Med. Chem.
92
78-90
2014
-
-
-
-
-
-
12
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
-
2
-
-
-
1
-
-
-
2
14
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
719064
Green
Inhibitors of acetyltransferas ...
Escherichia coli, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae
Bioorg. Med. Chem. Lett.
22
1510-1519
2012
-
-
-
2
-
-
156
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
4
4
4
-
-
-
4
-
-
-
-
-
150
-
-
-
-
2
-
-
150
156
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
4
4
-
-
-
4
-
-
-
-
4
4
-
-
-
719070
Stokes
Inhibitors of the acetyltransf ...
Escherichia coli, Haemophilus influenzae, Streptococcus pneumoniae
Bioorg. Med. Chem. Lett.
22
7019-7023
2012
-
-
-
-
-
-
54
-
-
-
-
3
-
5
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
52
-
-
-
-
-
-
-
52
54
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719137
Rodriguez-Diaz
Metabolic engineering of Lacto ...
Lactobacillus casei
Biotechnol. Bioeng.
109
1704-1712
2012
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
719411
Min
Structure-based virtual screen ...
Xanthomonas oryzae
Eur. J. Med. Chem.
53
150-158
2012
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
719574
Zhou
Identification of amino acids ...
Mycobacterium tuberculosis
Glycoconj. J.
29
297-303
2012
-
-
1
-
10
-
-
14
-
-
1
1
-
3
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
10
-
-
-
-
14
-
-
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
720042
Jagtap
Substrate bound crystal struct ...
Mycobacterium tuberculosis
J. Biol. Chem.
287
39524-39537
2012
1
1
-
1
13
-
-
6
-
-
-
4
-
2
-
1
-
1
-
-
-
-
6
1
1
-
-
-
1
-
-
1
-
-
-
1
2
-
1
2
20
-
-
-
-
6
-
-
-
4
-
-
1
-
-
-
-
-
6
2
2
-
-
-
2
-
-
-
-
5
7
-
-
-
720872
Singh
Kinetic modelling of GlmU reac ...
Mycobacterium tuberculosis
PLoS ONE
7
e43969
2012
-
1
-
-
-
-
1
5
-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
5
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
718708
Zhou
Kinetic properties of Mycobact ...
Mycobacterium tuberculosis
Arch. Microbiol.
193
751-757
2011
-
1
-
-
-
-
-
3
-
1
-
2
-
3
-
-
-
-
-
-
-
-
2
-
1
1
-
2
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
1
-
2
-
-
-
-
-
-
-
-
2
-
1
1
-
2
1
1
-
-
-
1
1
-
-
-
719224
Li
Design and synthesis of novel ...
Mycobacterium tuberculosis
Carbohydr. Res.
346
1714-1720
2011
-
-
1
-
-
-
2
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
719986
Buurman
In vitro validation of acetylt ...
Escherichia coli, Escherichia coli ATCC 27325, Haemophilus influenzae, Haemophilus influenzae ATCC 51907, Staphylococcus aureus, Staphylococcus aureus RN4220, Streptococcus pneumoniae, Streptococcus pneumoniae NCTC 7466
J. Biol. Chem.
286
40734-40742
2011
-
4
4
-
-
-
25
-
-
-
-
8
-
28
-
-
4
-
-
-
-
-
8
-
-
-
-
-
4
-
-
-
1
-
20
-
4
4
-
-
-
-
20
25
1
-
-
-
-
8
-
-
-
4
-
-
-
-
8
-
-
-
-
-
4
-
-
-
-
4
4
-
-
-
725249
Zhang
Identification of novel acetyl ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7
J. Bacteriol.
192
3287-3293
2010
-
-
-
-
-
-
5
2
-
1
-
2
-
7
-
-
1
1
-
-
1
-
4
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
2
-
1
-
2
-
-
-
1
-
-
1
-
4
-
1
-
-
1
1
-
-
-
-
1
1
-
1
1
701468
Zhang
Structure and function of GlmU ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Acta Crystallogr. Sect. D
65
275-283
2009
1
-
1
1
-
-
-
-
-
-
-
-
-
9
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
701520
Verma
Structure of N-acetylglucosami ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Acta Crystallogr. Sect. F
65
435-439
2009
-
-
1
1
-
-
-
-
-
-
-
-
-
10
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
705135
Parikh
PknB-mediated phosphorylation ...
Mycobacterium tuberculosis
J. Mol. Biol.
386
451-464
2009
-
-
1
1
7
-
-
2
-
-
-
-
-
2
-
1
-
-
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
7
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
687124
Zhang
Expression, essentiality, and ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Int. J. Biochem. Cell Biol.
40
2560-2571
2008
-
1
1
-
-
-
-
-
-
-
1
-
-
13
-
-
1
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
706622
Mochalkin
Structure of a small-molecule ...
Mycobacterium tuberculosis
Protein Sci.
17
577-582
2008
-
-
1
1
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677026
Olsen
Structure of the E. coli bifun ...
Escherichia coli
Protein Sci.
16
1230-1235
2007
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288687
Olsen
Purification, crystallization ...
Escherichia coli
Acta Crystallogr. Sect. D
57
296-297
2001
-
-
1
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288688
Pompeo
Dissection of the bifunctional ...
Escherichia coli, Escherichia coli JM83
J. Biol. Chem.
276
3833-3839
2001
-
-
1
1
-
-
-
-
-
-
3
2
-
10
-
-
1
-
-
-
5
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
2
-
-
-
1
-
-
5
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643073
Sulzenbacher
Crystal structure of Streptoco ...
Streptococcus pneumoniae
J. Biol. Chem.
276
11844-11851
2001
-
-
-
1
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643075
Olsen
Structure of the Escherichia c ...
Escherichia coli
Biochemistry
40
1913-1921
2001
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643076
Kostrewa
Crystal structures of Streptoc ...
Streptococcus pneumoniae
J. Mol. Biol.
305
279-289
2001
-
-
-
1
-
-
-
-
-
1
-
1
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288686
Pompeo
Probing the role of cysteine r ...
Bacillus subtilis, Escherichia coli, Escherichia coli JM83, Neisseria gonorrhoeae
J. Bacteriol.
180
4799-4803
1998
-
-
-
-
-
1
5
10
-
-
-
4
-
12
-
-
1
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
5
-
10
-
-
-
4
-
-
-
1
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288685
Mengin-Lecreulx
Copurification of glucosamine- ...
Bacillus subtilis, Escherichia coli, Escherichia coli JM83
J. Bacteriol.
176
5788-5795
1994
-
-
-
-
-
1
4
4
1
1
1
3
-
11
-
-
1
1
-
-
2
-
8
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
4
1
1
1
3
-
-
-
1
-
-
2
-
8
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-