BRENDA - Enzyme Database
show all sequences of 2.3.1.157

Structure of the E. coli bifunctional GlmU acetyltransferase active site with substrates and products

Olsen, L.R.; Vetting, M.W.; Roderick, S.L.; Protein Sci. 16, 1230-1235 (2007)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
in complex with acetyl-CoA, CoA and glucosamine-1-phosphate, and with desulfo-CoA and N-acetylglucosamine-1-phosphate. The 2-amino group of glucosamine-1-phosphate is positioned in proximity to the acetyl-CoA to facilitate direct attack on its thioester by a ternary complex mechanism
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Reaction
Reaction
Commentary
Organism
acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
the 2-amino group of glucosamine-1-phosphate is positioned in proximity to the acetyl-CoA to facilitate direct attack on its thioester by a ternary complex mechanism
Escherichia coli
Crystallization (Commentary) (protein specific)
Crystallization
Organism
in complex with acetyl-CoA, CoA and glucosamine-1-phosphate, and with desulfo-CoA and N-acetylglucosamine-1-phosphate. The 2-amino group of glucosamine-1-phosphate is positioned in proximity to the acetyl-CoA to facilitate direct attack on its thioester by a ternary complex mechanism
Escherichia coli
Other publictions for EC 2.3.1.157
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746844
Honda
Improvement of ST0452 N-acety ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii DSM 16993
Appl. Environ. Microbiol.
84
e002213-18
2018
-
-
1
1
2
-
-
-
-
-
-
-
-
10
-
-
1
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
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-
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1
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1
2
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1
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-
-
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-
-
-
-
-
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-
-
-
-
-
-
-
-
736517
Sharma
Escherichia coli N-acetylgluco ...
Escherichia coli, Escherichia coli ATCC 25922
J. Biomol. Screen.
21
342-353
2016
-
-
1
-
-
-
3
-
-
1
-
2
-
10
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
4
-
2
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1
1
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2
4
4
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1
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2
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1
-
-
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-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
737096
Dziadek
Mycobacterium tuberculosis Ats ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis ATCC 25618, Mycobacterium tuberculosis H37Rv
PLoS ONE
11
e0148030
2016
-
1
1
-
1
-
-
-
-
-
-
3
-
15
-
-
1
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6
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1
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1
1
1
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1
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3
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1
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-
6
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
729696
Zhang
Characterization of the amino ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7, Sulfurisphaera tokodaii DSM 16993 / JCM 10545 / NBRC 100140 / 7
Extremophiles
19
417-427
2015
-
1
1
-
7
-
1
9
-
1
-
6
-
8
-
-
1
-
-
-
12
-
9
2
1
-
1
6
1
-
-
1
-
-
-
-
1
1
1
-
7
-
-
1
-
9
-
1
-
6
-
-
-
1
-
-
12
-
9
2
1
-
1
6
1
-
-
-
-
2
2
-
6
6
735518
Sharma
Identification and characteriz ...
Escherichia coli, Escherichia coli ATCC 25922, Haemophilus influenzae, Haemophilus influenzae ATCC 51907
Appl. Microbiol. Biotechnol.
100
3071-3085
2015
-
-
1
-
-
-
15
-
-
-
-
8
-
12
-
-
1
-
-
-
-
-
10
-
2
-
-
-
2
-
-
2
2
-
11
-
-
1
2
-
-
-
12
17
2
-
-
-
-
8
-
-
-
1
-
-
-
-
10
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
735569
Patin
Purification and biochemical c ...
Yersinia pestis, Yersinia pestis YPIII, Yersinia pseudotuberculosis, Yersinia pseudotuberculosis YPIII
Arch. Microbiol.
197
371-378
2015
1
-
2
-
1
-
2
4
-
2
-
2
-
6
-
-
1
-
-
-
-
-
4
2
1
-
-
4
1
-
-
1
-
-
-
1
-
2
1
-
1
-
-
2
-
4
-
2
-
2
-
-
-
1
-
-
-
-
4
2
1
-
-
4
1
-
-
-
-
2
2
-
-
-
737150
Soni
Depletion of M. tuberculosis G ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis ATCC 25618, Mycobacterium tuberculosis H37Rv
PLoS Pathog.
11
e1005235
2015
-
1
1
1
-
-
2
-
-
-
-
6
-
12
-
-
-
-
-
-
-
-
6
-
1
-
-
-
1
-
-
1
-
-
1
-
1
1
1
1
-
-
1
2
-
-
-
-
-
6
-
-
-
-
-
-
-
-
6
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
737291
Rani
High-throughput screen identif ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Tuberculosis
95
664-677
2015
-
-
1
-
-
-
6
3
-
1
-
4
-
11
-
-
1
-
-
-
-
-
5
-
1
-
-
-
1
-
-
1
5
-
10
-
-
1
1
-
-
-
10
8
5
5
-
1
-
4
-
-
-
1
-
-
-
-
5
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
735396
Vithani
GlmU (N-acetylglucosamine-1-ph ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis ATCC 25618
Acta Crystallogr. Sect. F
70
703-708
2014
-
-
1
1
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
736058
Mehra
Identification and optimizatio ...
Escherichia coli, Escherichia coli ATCC 25922
Eur. J. Med. Chem.
92
78-90
2014
-
-
-
-
-
-
12
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
-
2
-
-
-
1
-
-
-
2
14
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
719064
Green
Inhibitors of acetyltransferas ...
Escherichia coli, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae
Bioorg. Med. Chem. Lett.
22
1510-1519
2012
-
-
-
2
-
-
156
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
4
4
4
-
-
-
4
-
-
-
-
-
150
-
-
-
-
2
-
-
150
156
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
4
4
-
-
-
4
-
-
-
-
4
4
-
-
-
719070
Stokes
Inhibitors of the acetyltransf ...
Escherichia coli, Haemophilus influenzae, Streptococcus pneumoniae
Bioorg. Med. Chem. Lett.
22
7019-7023
2012
-
-
-
-
-
-
54
-
-
-
-
3
-
5
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
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-
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-
52
-
-
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-
-
52
54
-
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-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719137
Rodriguez-Diaz
Metabolic engineering of Lacto ...
Lactobacillus casei
Biotechnol. Bioeng.
109
1704-1712
2012
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
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-
1
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-
1
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1
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-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
719411
Min
Structure-based virtual screen ...
Xanthomonas oryzae
Eur. J. Med. Chem.
53
150-158
2012
-
-
-
-
-
-
-
-
-
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-
1
-
3
-
-
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1
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1
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1
-
-
-
-
-
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-
1
1
-
-
-
719574
Zhou
Identification of amino acids ...
Mycobacterium tuberculosis
Glycoconj. J.
29
297-303
2012
-
-
1
-
10
-
-
14
-
-
1
1
-
3
-
-
1
-
-
-
-
-
1
1
-
-
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-
-
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1
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10
-
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14
-
-
1
1
-
-
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1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
720042
Jagtap
Substrate bound crystal struct ...
Mycobacterium tuberculosis
J. Biol. Chem.
287
39524-39537
2012
1
1
-
1
13
-
-
6
-
-
-
4
-
2
-
1
-
1
-
-
-
-
6
1
1
-
-
-
1
-
-
1
-
-
-
1
2
-
1
2
20
-
-
-
-
6
-
-
-
4
-
-
1
-
-
-
-
-
6
2
2
-
-
-
2
-
-
-
-
5
7
-
-
-
720872
Singh
Kinetic modelling of GlmU reac ...
Mycobacterium tuberculosis
PLoS ONE
7
e43969
2012
-
1
-
-
-
-
1
5
-
-
-
1
-
1
-
-
-
1
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1
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1
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1
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1
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1
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5
-
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1
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-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
718708
Zhou
Kinetic properties of Mycobact ...
Mycobacterium tuberculosis
Arch. Microbiol.
193
751-757
2011
-
1
-
-
-
-
-
3
-
1
-
2
-
3
-
-
-
-
-
-
-
-
2
-
1
1
-
2
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
1
-
2
-
-
-
-
-
-
-
-
2
-
1
1
-
2
1
1
-
-
-
1
1
-
-
-
719224
Li
Design and synthesis of novel ...
Mycobacterium tuberculosis
Carbohydr. Res.
346
1714-1720
2011
-
-
1
-
-
-
2
-
-
-
-
2
-
1
-
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2
-
1
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1
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1
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2
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2
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2
-
1
-
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-
1
-
-
-
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-
-
-
-
719986
Buurman
In vitro validation of acetylt ...
Escherichia coli, Escherichia coli ATCC 27325, Haemophilus influenzae, Haemophilus influenzae ATCC 51907, Staphylococcus aureus, Staphylococcus aureus RN4220, Streptococcus pneumoniae, Streptococcus pneumoniae NCTC 7466
J. Biol. Chem.
286
40734-40742
2011
-
4
4
-
-
-
25
-
-
-
-
8
-
28
-
-
4
-
-
-
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8
-
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-
4
-
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1
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20
-
4
4
-
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20
25
1
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8
-
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4
-
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-
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8
-
-
-
-
-
4
-
-
-
-
4
4
-
-
-
725249
Zhang
Identification of novel acetyl ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7
J. Bacteriol.
192
3287-3293
2010
-
-
-
-
-
-
5
2
-
1
-
2
-
7
-
-
1
1
-
-
1
-
4
-
1
-
-
1
1
-
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5
-
2
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1
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2
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1
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1
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4
-
1
-
-
1
1
-
-
-
-
1
1
-
1
1
701468
Zhang
Structure and function of GlmU ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Acta Crystallogr. Sect. D
65
275-283
2009
1
-
1
1
-
-
-
-
-
-
-
-
-
9
-
-
1
-
-
-
-
-
2
-
1
-
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1
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-
1
-
1
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1
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1
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-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
701520
Verma
Structure of N-acetylglucosami ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Acta Crystallogr. Sect. F
65
435-439
2009
-
-
1
1
-
-
-
-
-
-
-
-
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10
-
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1
-
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1
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
705135
Parikh
PknB-mediated phosphorylation ...
Mycobacterium tuberculosis
J. Mol. Biol.
386
451-464
2009
-
-
1
1
7
-
-
2
-
-
-
-
-
2
-
1
-
-
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
7
-
-
-
-
2
-
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-
-
-
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1
-
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
687124
Zhang
Expression, essentiality, and ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Int. J. Biochem. Cell Biol.
40
2560-2571
2008
-
1
1
-
-
-
-
-
-
-
1
-
-
13
-
-
1
1
-
-
-
-
4
-
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1
1
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1
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1
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4
-
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-
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-
-
-
-
-
-
-
-
-
-
-
706622
Mochalkin
Structure of a small-molecule ...
Mycobacterium tuberculosis
Protein Sci.
17
577-582
2008
-
-
1
1
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
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