Cloned (Comment) | Organism |
---|---|
gene Tb10.70.4400, sequence comaprisons, TbGAT restores glycerol-3-phosphate acyltransferase activity when expressed in a Leishmania major deletion strain lacking this activity and exhibits preference for medium length, unsaturated fatty acyl-CoAs | Trypanosoma brucei brucei |
Protein Variants | Comment | Organism |
---|---|---|
additional information | creation of the null mutant DELTAtbgat/DELTA tbgat and complementation of lines DELTA tbgat/DELTA tbgat/TbGAT and DELTA tbgat/DELTA tbgat/HV:TbGAT. The null mutant of TbGAT is created by sequential replacement of both alleles with first a BSD and then a HYG cassette, conferring blasticidin and hygromycin resistance, respectively, geno- and phenotypes, overview | Trypanosoma brucei brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum membrane | TbGAT localizes to the endoplasmic reticulum membrane with its N-terminal domain facing the cytosol | Trypanosoma brucei brucei | 5789 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | Trypanosoma brucei brucei | - |
CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | Trypanosoma brucei brucei 927/4 GUTat10.1 | - |
CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei brucei | Q38BT6 | - |
- |
Trypanosoma brucei brucei 927/4 GUTat10.1 | Q38BT6 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | procyclic form | Trypanosoma brucei brucei | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | - |
Trypanosoma brucei brucei | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | the enzyme exhibits preference for medium length, unsaturated fatty acyl-CoAs, i.e. palmitoyl-CoA | Trypanosoma brucei brucei | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | - |
Trypanosoma brucei brucei 927/4 GUTat10.1 | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | the enzyme exhibits preference for medium length, unsaturated fatty acyl-CoAs, i.e. palmitoyl-CoA | Trypanosoma brucei brucei 927/4 GUTat10.1 | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + sn-glycerol 3-phosphate | - |
Trypanosoma brucei brucei | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + sn-glycerol 3-phosphate | - |
Trypanosoma brucei brucei 927/4 GUTat10.1 | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | TbGAT (gene ID Tb927.10.3100) encodes a protein of 698 amino acids with three putative C-terminal hydrophobic spans. No signal peptide can be predicted | Trypanosoma brucei brucei |
Synonyms | Comment | Organism |
---|---|---|
glycerol-3-phosphate acyltransferase | - |
Trypanosoma brucei brucei |
TbGAT | - |
Trypanosoma brucei brucei |
General Information | Comment | Organism |
---|---|---|
malfunction | the enzyme null mutant exhibits a slight decrease in phosphatidylethanolamine biosynthesis that is compensated with a modest increase in production of ether phosphatidylcholine. TbGAT null mutant in Trypanosoma brucei procyclic forms lacks glycerol-3-phosphate acyltransferase activity, but remains viable and exhibits similar growth rate as the wild-type | Trypanosoma brucei brucei |
physiological function | the biosynthesis of ester glycerolipids initiates with the stepwise addition of fatty acyl groups to glycerol-3-phosphate (G3P). The first fatty acyl group is added at the sn-1 position by a G3P acyltransferase (GPAT) to give 1-acyl-G3P or lysophosphatidic acid (LPA), while the second fatty acyl group is added to the sn-2 position by a 1-acyl-G3P acyltransferase to give phosphatidic acid (PA). The glycerol-3-phosphate acyltransferase TbGAT is dispensable for viability and the biosynthesis of glycerolipids, i.e. phosphatidylcholine, phosphatidylinositol, phosphatidylserine, and GPI-anchored protein procyclin, in Trypanosoma brucei. Recombinant TbGAT restores glycerol-3-phosphate acyltransferase activity when expressed in a Leishmania major deletion strain lacking this activity and exhibits preference for medium length, unsaturated fatty acyl-CoAs. Gene TbGAT encodes a GPAT enzyme with broad fatty acyl-CoA specificity but preference for oleoyl-CoA | Trypanosoma brucei brucei |