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Literature summary for 2.3.1.15 extracted from
- Cloning, heterologous expression and biochemical characterization of plastidial sn-glycerol-3-phosphate acyltransferase from Helianthus annuus (2015), Phytochemistry, 111, 27-36.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli XL1-Blue cells | Helianthus annuus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00196 | - |
palmitoyl-[acyl-carrier protein] | at pH 7.4 and 25°C | Helianthus annuus |  |
| 0.00612 | - |
stearoyl-[acyl-carrier protein] | at pH 7.4 and 25°C | Helianthus annuus | |
| 0.0078 | - |
oleoyl-[acyl-carrier protein] | at pH 7.4 and 25°C | Helianthus annuus | |
| 0.659 | - |
sn-glycerol 3-phosphate | at pH 7.4 and 25°C | Helianthus annuus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Helianthus annuus | 9507 | - |
| plastid | - |
Helianthus annuus | 9536 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
x * 50000, His-tagged enzyme, SDS-PAGE | Helianthus annuus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helianthus annuus | K9JTB0 | line CAS-6 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His SpinTrap column chromatography | Helianthus annuus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Helianthus annuus | - |
| leaf | - |
Helianthus annuus | - |
| stem | - |
Helianthus annuus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oleoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate | most active acyl donor | Helianthus annuus | acyl-carrier protein + 1-oleoyl-sn-glycerol 3-phosphate | - |
? | |
| palmitoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate | - |
Helianthus annuus | acyl-carrier protein + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
| stearoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate | - |
Helianthus annuus | acyl-carrier protein + 1-stearoyl-sn-glycerol 3-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, His-tagged enzyme, SDS-PAGE | Helianthus annuus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acyl-[acyl carrier protein]:sn-1-glycerol-3-phosphate acyltransferase | - |
Helianthus annuus |
| GPAT | - |
Helianthus annuus |
| PlsB | - |
Helianthus annuus |
| sn-glycerol-3-phosphate acyltransferase | - |
Helianthus annuus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00674 | - |
stearoyl-[acyl-carrier protein] | at pH 7.4 and 25°C | Helianthus annuus | |
| 0.00944 | - |
palmitoyl-[acyl-carrier protein] | at pH 7.4 and 25°C | Helianthus annuus | |
| 0.011 | - |
sn-glycerol 3-phosphate | at pH 7.4 and 25°C | Helianthus annuus | |
| 0.0918 | - |
oleoyl-[acyl-carrier protein] | at pH 7.4 and 25°C | Helianthus annuus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | - |
- |
Helianthus annuus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.017 | - |
sn-glycerol 3-phosphate | at pH 7.4 and 25°C | Helianthus annuus | |
| 1.1 | - |
stearoyl-[acyl-carrier protein] | at pH 7.4 and 25°C | Helianthus annuus | |
| 4.82 | - |
palmitoyl-[acyl-carrier protein] | at pH 7.4 and 25°C | Helianthus annuus | |
| 11.8 | - |
oleoyl-[acyl-carrier protein] | at pH 7.4 and 25°C | Helianthus annuus | |
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