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Literature summary for 2.3.1.15 extracted from

  • Ruiz-Lopez, N.; Garces, R.; Harwood, J.L.; Martinez-Force, E.
    Characterization and partial purification of acyl-CoA:glycerol 3-phosphate acyltransferase from sunflower (Helianthus annuus L.) developing seeds (2009), Plant Physiol. Biochem., 48, 73-80.
    View publication on PubMed
Search for more literature for 2.3.1.15

Activating Compound

Activating Compound Comment Organism Structure
EDTA 10 mM, enhances activity, relative activity: 143% Helianthus annuus

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ 2 mM, 87% residual activity; slightly decreases activity, relative activity: 87.5% (2 mM), 37% (5 mM) Helianthus annuus
Mn2+ 2 mM, 82% residaul activity; slightly decreases activity, relative activity: 81.5% (2 mM) Helianthus annuus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.354
-
 glycerol 3-phosphate
-
 Helianthus annuus
0.354
-
 glycerol 3-phosphate pH 7.5, 30°C Helianthus annuus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Helianthus annuus
-
-
microsome GPAT is prepared from microsomes Helianthus annuus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60100
-
 SDS-PAGE Helianthus annuus

Organism

Organism UniProt Comment Textmining
Helianthus annuus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
high solubilisation is achieved using 0.75% Tween80 and the solubilised GPAT is partially purified by ion-exchange chromatography using a Hi-Trap DEAE FF column, followed by gel filtration chromatography using a Superose 12 HR column Helianthus annuus
partial Helianthus annuus

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Helianthus annuus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 maximum value for GPAT activity identified between 15 and 20 days after flowering Helianthus annuus
0.037
-
 pH 7.5, 30°C Helianthus annuus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
linoleoyl-CoA + glycerol 3-phosphate preferred substrate linoleoyl-CoA (equal to palmitoyl-CoA) Helianthus annuus CoA + 1-linoleoyl-glycerol 3-phosphate
-
 ?
linoleoyl-CoA + glycerol 3-phosphate and palmitoyl-CoA, best substrates Helianthus annuus CoA + 1-linoleoyl-glycerol 3-phosphate
-
 ?
oleoyl-CoA + glycerol 3-phosphate
-
 Helianthus annuus CoA + 1-oleoyl-glycerol 3-phosphate
-
 ?
oleoyl-CoA + glycerol 3-phosphate moderate activity with oleoyl-CoA Helianthus annuus CoA + 1-oleoyl-glycerol 3-phosphate
-
 ?
palmitoyl-CoA + glycerol 3-phosphate preferred substrate palmitoyl-CoA (equal to linoleoyl-CoA) Helianthus annuus CoA + 1-palmitoyl-glycerol 3-phosphate
-
 ?
palmitoyl-CoA + glycerol 3-phosphate and lineoyl-CoA, best substrates Helianthus annuus CoA + 1-palmitoyl-glycerol 3-phosphate
-
 ?
stearoyl-CoA + glycerol 3-phosphate
-
 Helianthus annuus CoA + 1-stearoyl-glycerol 3-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
glycerol-3-phosphate acyltransferase
-
 Helianthus annuus
GPAT
-
 Helianthus annuus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Helianthus annuus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Helianthus annuus
7.5
-
 assay at and optimal pH Helianthus annuus