Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | alleviates detergent effect of palmitoyl-CoA | Escherichia coli | |
bovine serum albumin | not necessary with acyl-ACP derivatives or mixed micellar samples | Escherichia coli | |
bovine serum albumin | alleviates detergent effect of palmitoyl-CoA and oleoyl-CoA | Escherichia coli | |
phosphatidylethanolamine | inefficient, pH-dependent | Escherichia coli | |
phosphatidylglycerol | - |
Escherichia coli | |
Phospholipids | phosphatidylethanolamine/phosphatidylglycerol/cardiolipin, ratio 6/1/1 | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
plsB structural gene encoding glycerol 3-phosphate acyltransferase | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | - |
Escherichia coli | 5743 | - |
plasma membrane | cytoplasmic side | Escherichia coli | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not activated by divalent ions | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | Escherichia coli | initial step in membrane phospholipid biosynthesis | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
overproducing strains VL3/pVL1, contains hybrid plasmid bearing plsB structural gene and VL3/plB3-4 | - |
Purification (Comment) | Organism |
---|---|
Matrex Gel Red A, octyl-Sepharose CL-4B, hydroxylapatite | Escherichia coli |
solubilized, by non-ionic detergents | Escherichia coli |
solubilized, by non-ionic detergents and reconstituted in unilamellar phospholipid vesicles | Escherichia coli |
Renatured (Comment) | Organism |
---|---|
reconstituted in phospholipid vesicles containing phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglecerol in the approx. molar ratio of 6:1:1 | Escherichia coli |
reconstitution in cardiolipin/C12E8, i.e. non-ionic detergent, ratio 2:1 mixed micelles | Escherichia coli |
reconstitution in vesicles | Escherichia coli |
Storage Stability | Organism |
---|---|
-70°C, solubilized, indefinitely stable | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + sn-glycerol 3-phosphate | dihydroxyacetone phosphate, ethylene glycol phosphate and 1,3-propanediol phosphate can replace glycerol 3-phosphate | Escherichia coli | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | exclusively acylates glycerol 3-phosphate at the C-1-position | Escherichia coli | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-CoA + sn-glycerol 3-phosphate | initial step in membrane phospholipid biosynthesis | Escherichia coli | CoA + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
acyl-[acyl-carrier protein] + sn-glycerol 3-phosphate | - |
Escherichia coli | acyl-carrier protein + 1-acyl-sn-glycerol 3-phosphate | - |
? | |
cis-vaccenoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate | - |
Escherichia coli | acyl-carrier protein + 1-cis-vaccenoyl-sn-glycerol 3-phosphate | - |
? | |
oleoyl-CoA + sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-oleoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-[acyl-carrier protein] + sn-glycerol 3-phosphate | - |
Escherichia coli | acyl-carrier protein + 1-palmitoyl-sn-glycerol 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | - |
Escherichia coli |
More | membranes of overproducing Escherichia coli strain VL3/pLB3-4 contain predominantly the dimeric form | Escherichia coli |