BRENDA - Enzyme Database
show all sequences of 2.3.1.148

The role of transacylases in the metabolism of arachidonate and platelet activating factor

Snyder, F.; Lee, T.C.; Blank, M.L.; Prog. Lipid Res. 31, 65-86 (1992) View publication on PubMed

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Zn2+
strong
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0015
-
1-hexadecyl-2-lyso-glycerophosphocholine
-
Oryctolagus cuniculus
0.0038
-
1-acyl-2-lyso-glycerophosphocholine
-
Oryctolagus cuniculus
0.017
-
1-palmitoyl-2-lyso-glycerophosphocholine
-
Rattus norvegicus
0.076
-
lyso-phosphatidylserine
-
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Rattus norvegicus
-
-
microsome
-
Oryctolagus cuniculus
-
-
microsome
-
Canis lupus familiaris
-
-
Organism
Organism
UniProt
Commentary
Textmining
Canis lupus familiaris
-
-
-
Homo sapiens
-
-
-
Mus musculus
-
-
-
Oryctolagus cuniculus
-
-
-
Rattus norvegicus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
alveolar macrophage
-
Oryctolagus cuniculus
-
heart
-
Canis lupus familiaris
-
liver
-
Rattus norvegicus
-
liver
-
Oryctolagus cuniculus
-
lung
-
Rattus norvegicus
-
lymphocyte
-
Mus musculus
-
macrophage
-
Mus musculus
-
platelet
-
Homo sapiens
-
platelet
-
Rattus norvegicus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1-Acyl-2-arachidonoyl-glycerophosphocholine + lyso-phosphatidylethanolamine
transfer of arachidonate occurs through a bidirectional movement
16278
Mus musculus
1-Acyl-2-lyso-glycerophosphocholine + arachidonoylphosphatidylethanolamine
-
-
-
?
1-acyl-2-arachidonoyl-sn-glycero-3-phosphoinositol + lyso-phosphatidylcholine
-
16278
Mus musculus
1-acyl-2-lyso-sn-glycero-3-phosphoinositol + ?
-
-
-
?
1-Acyl-2-arachidonoyl-sn-glycero-3-phosphophoinositol + lyso-phosphatidylethanolamine
unidirectional transfer process of arachidonate
16278
Mus musculus
1-Acyl-2-lyso-sn-glycero-3-phosphoinositol + arachidonoylphosphatidylethanolamine
-
-
-
?
1-acyl-2-lyso-glycerophosphocholine + arachidonoylphosphatidylethanolamine
-
16278
Oryctolagus cuniculus
1-acyl-2-arachidonoyl-glycerophosphocholine + lyso-phosphatidylethanolamine
-
-
-
r
1-Hexadecyl-2-lyso-glycerophosphocholine + phospholipid
-
16278
Oryctolagus cuniculus
?
-
-
-
?
1-palmitoyl-2-lyso-glycerophosphocholine + arachidonoylphosphatidylethanolamine
-
16278
Rattus norvegicus
1-palmitoyl-2-arachidonoyl-glycerophosphocholine + lyso-phosphatidylethanolamine
-
-
-
r
lyso-Pasmenylethanolamine + phospholipid
-
16278
Homo sapiens
?
-
-
-
?
lyso-Phosphatidylethanolamine + phospholipid
-
16278
Homo sapiens
Phosphatidylethanolamine + lyso-phospholipid
-
-
-
?
lyso-Phosphatidylinositol + phospholipid
-
16278
Homo sapiens
Phosphatidylinositol + lyso-phospholipid
-
-
-
?
lyso-Phosphatidylserine + phospholipid
-
16278
Homo sapiens
Phosphatidylserine + lyso-phospholipid
-
-
-
?
additional information
arachidonate and linoleate at the sn-2 position of phosphatidylcholine can be transferred to lyso-phosphatidylethanolamine. The transfer of 16:0, 18:0, and 18:1 acyl moieties at the sn-2-position of phosphatidylcholine is negligible
16278
Rattus norvegicus
?
-
-
-
?
additional information
more efficient transfer of arachidonate from phosphatidylcholine to the ethanolamine-containing phospholipids than from phosphatidylinositol
16278
Homo sapiens
?
-
-
-
?
Phosphatidylcholine + lyso-phosphatidylethanolamine
-
16278
Mus musculus
lyso-Phosphatidylcholine + phosphatidylethanolamine
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.5
-
bimodal pH-optimum, at pH 4.5 and at pH 7.5. The activity is 4-5times higher at pH 4.5 than at pH 7.5
Canis lupus familiaris
7.5
-
-
Rattus norvegicus
7.5
-
bimodal pH-optimum, at pH 7.5 and at pH 4.5
Canis lupus familiaris
Cofactor
Cofactor
Commentary
Organism
Structure
CoA
-
Canis lupus familiaris
CoA
required
Mus musculus
CoA
required
Homo sapiens
CoA
required
Rattus norvegicus
CoA
required
Oryctolagus cuniculus
CoA
Km: 0.0015 mM, lung enzyme, with lyso-phosphatidylethanolamine as substrate
Rattus norvegicus
CoA
Km: 0.014 mM, liver enzyme, with 2-lyso-sn-phosphatidylinositol as substrate
Rattus norvegicus
CoA
Km: 0.0014 mM
Homo sapiens
CoA
Km: 0.0014 mM
Oryctolagus cuniculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
CoA
-
Canis lupus familiaris
CoA
required
Mus musculus
CoA
required
Homo sapiens
CoA
required
Rattus norvegicus
CoA
required
Oryctolagus cuniculus
CoA
Km: 0.0015 mM, lung enzyme, with lyso-phosphatidylethanolamine as substrate
Rattus norvegicus
CoA
Km: 0.014 mM, liver enzyme, with 2-lyso-sn-phosphatidylinositol as substrate
Rattus norvegicus
CoA
Km: 0.0014 mM
Homo sapiens
CoA
Km: 0.0014 mM
Oryctolagus cuniculus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Zn2+
strong
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0015
-
1-hexadecyl-2-lyso-glycerophosphocholine
-
Oryctolagus cuniculus
0.0038
-
1-acyl-2-lyso-glycerophosphocholine
-
Oryctolagus cuniculus
0.017
-
1-palmitoyl-2-lyso-glycerophosphocholine
-
Rattus norvegicus
0.076
-
lyso-phosphatidylserine
-
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Rattus norvegicus
-
-
microsome
-
Oryctolagus cuniculus
-
-
microsome
-
Canis lupus familiaris
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
alveolar macrophage
-
Oryctolagus cuniculus
-
heart
-
Canis lupus familiaris
-
liver
-
Rattus norvegicus
-
liver
-
Oryctolagus cuniculus
-
lung
-
Rattus norvegicus
-
lymphocyte
-
Mus musculus
-
macrophage
-
Mus musculus
-
platelet
-
Homo sapiens
-
platelet
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1-Acyl-2-arachidonoyl-glycerophosphocholine + lyso-phosphatidylethanolamine
transfer of arachidonate occurs through a bidirectional movement
16278
Mus musculus
1-Acyl-2-lyso-glycerophosphocholine + arachidonoylphosphatidylethanolamine
-
-
-
?
1-acyl-2-arachidonoyl-sn-glycero-3-phosphoinositol + lyso-phosphatidylcholine
-
16278
Mus musculus
1-acyl-2-lyso-sn-glycero-3-phosphoinositol + ?
-
-
-
?
1-Acyl-2-arachidonoyl-sn-glycero-3-phosphophoinositol + lyso-phosphatidylethanolamine
unidirectional transfer process of arachidonate
16278
Mus musculus
1-Acyl-2-lyso-sn-glycero-3-phosphoinositol + arachidonoylphosphatidylethanolamine
-
-
-
?
1-acyl-2-lyso-glycerophosphocholine + arachidonoylphosphatidylethanolamine
-
16278
Oryctolagus cuniculus
1-acyl-2-arachidonoyl-glycerophosphocholine + lyso-phosphatidylethanolamine
-
-
-
r
1-Hexadecyl-2-lyso-glycerophosphocholine + phospholipid
-
16278
Oryctolagus cuniculus
?
-
-
-
?
1-palmitoyl-2-lyso-glycerophosphocholine + arachidonoylphosphatidylethanolamine
-
16278
Rattus norvegicus
1-palmitoyl-2-arachidonoyl-glycerophosphocholine + lyso-phosphatidylethanolamine
-
-
-
r
lyso-Pasmenylethanolamine + phospholipid
-
16278
Homo sapiens
?
-
-
-
?
lyso-Phosphatidylethanolamine + phospholipid
-
16278
Homo sapiens
Phosphatidylethanolamine + lyso-phospholipid
-
-
-
?
lyso-Phosphatidylinositol + phospholipid
-
16278
Homo sapiens
Phosphatidylinositol + lyso-phospholipid
-
-
-
?
lyso-Phosphatidylserine + phospholipid
-
16278
Homo sapiens
Phosphatidylserine + lyso-phospholipid
-
-
-
?
additional information
arachidonate and linoleate at the sn-2 position of phosphatidylcholine can be transferred to lyso-phosphatidylethanolamine. The transfer of 16:0, 18:0, and 18:1 acyl moieties at the sn-2-position of phosphatidylcholine is negligible
16278
Rattus norvegicus
?
-
-
-
?
additional information
more efficient transfer of arachidonate from phosphatidylcholine to the ethanolamine-containing phospholipids than from phosphatidylinositol
16278
Homo sapiens
?
-
-
-
?
Phosphatidylcholine + lyso-phosphatidylethanolamine
-
16278
Mus musculus
lyso-Phosphatidylcholine + phosphatidylethanolamine
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.5
-
bimodal pH-optimum, at pH 4.5 and at pH 7.5. The activity is 4-5times higher at pH 4.5 than at pH 7.5
Canis lupus familiaris
7.5
-
-
Rattus norvegicus
7.5
-
bimodal pH-optimum, at pH 7.5 and at pH 4.5
Canis lupus familiaris
Other publictions for EC 2.3.1.148
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
16278
Snyder
The role of transacylases in t ...
Canis lupus familiaris, Oryctolagus cuniculus, Homo sapiens, Mus musculus, Rattus norvegicus
Prog. Lipid Res.
31
65-86
1992
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1
4
3
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5
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9
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13
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3
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9
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9
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1
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4
3
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9
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13
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3
-
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-
-
-
-
-
16275
Robinson
Acylation of lysophospholipids ...
Oryctolagus cuniculus
J. Biol. Chem.
260
7889-7895
1985
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1
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1
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1
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1
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1
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1
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1
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1
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1
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-
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-
-
-
-
-
16276
Irvine
Transfer of arachidonic acid b ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
91
1399-1405
1979
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1
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1
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1
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2
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2
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1
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1
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2
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