Literature summary for 2.3.1.144 extracted from

Eudes, A.; Juminaga, D.; Baidoo, E.E.; Collins, F.W.; Keasling, J.D.; Loque, D.
Production of hydroxycinnamoyl anthranilates from glucose in Escherichia coli (2013), Microb. Cell Fact., 12, 62.

Search for more literature for 2.3.1.144

Application

Application	Comment	Organism
synthesis	coexpression of HCBT and 4-coumarate:CoA 4CL1 ligase from Nicotiana tabacum in the Escherichia coli anthranilate-accumulating strain W3110 trpD9923 allows the production of Avn D [N-(4'-hydroxycinnamoyl)-anthranilic acid] and Avn F [N-(3',4'-dihydroxycinnamoyl)-anthranilic acid] upon feeding with p-coumarate and caffeate, respectively. Additional expression of a tyrosine ammonia-lyase from Rhodotorula glutinis (TAL) leads to the conversion of endogenous tyrosine into p-coumarate and results in the production of Avn D [N-(4'-hydroxycinnamoyl)-anthranilic acid] from glucose. A 135fold improvement in Avn D titer is achieved by boosting tyrosine production. Expression of either the p-coumarate 3-hydroxylase Sam5 from Saccharothrix espanensis or the hydroxylase complex HpaBC from Escherichia coli results in the endogenous production of caffeate and biosynthesis of Avn F [N-(3',4'-dihydroxycinnamoyl)-anthranilic acid]	Dianthus caryophyllus

Organism

Organism	UniProt	Comment	Textmining
Dianthus caryophyllus	O24645	isoform HCBT1	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-coumaroyl-CoA + anthranilate	-	Dianthus caryophyllus	CoA + N-4-(coumaroyl)anthranilate	-	?
benzoyl-CoA + anthranilate	-	Dianthus caryophyllus	CoA + N-benzoylanthranilate	-	?

Synonyms

Synonyms	Comment	Organism
HCBT	-	Dianthus caryophyllus
hydroxycinnamoyl/benzoyl-CoA/anthranilate N-hydroxycinnamoyl/benzoyltransferase	-	Dianthus caryophyllus

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Release 2023.1 (January 2023)