Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Dianthus caryophyllus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
benzoyl-CoA | - |
Dianthus caryophyllus | |
0.02 | - |
anthranilate | - |
Dianthus caryophyllus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
gel filtration | Dianthus caryophyllus |
53000 | - |
1 * 53000, SDS-PAGE | Dianthus caryophyllus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dianthus caryophyllus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Q-Sepharose, Blue Sepharose, Sephacryl S200, Mono Q | Dianthus caryophyllus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00000948 | - |
- |
Dianthus caryophyllus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + anthranilate | 127% of activity with benzoyl-CoA | Dianthus caryophyllus | CoA + N-4-(coumaroyl)anthranilate | - |
? | |
benzoyl-CoA + anthranilate | cinnamoyl-CoA, salicyl-CoA and 4-coumaroyl-CoA can act as donors | Dianthus caryophyllus | CoA + N-benzoylanthranilate | - |
? | |
cinnamoyl-CoA + anthranilate | 140% of activity with benzoyl-CoA | Dianthus caryophyllus | CoA + N-cinnamoylanthranilate | - |
? | |
salicyl-CoA + anthranilate | 60% of activity with benzoyl-CoA | Dianthus caryophyllus | CoA + N-salicylanthranilate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 53000, SDS-PAGE | Dianthus caryophyllus |
Synonyms | Comment | Organism |
---|---|---|
anthranilate N-hydroxycinammoyl/benzoyltransferase | - |
Dianthus caryophyllus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7 | activity is completely lost if the pH drops below pH 6.5 | Dianthus caryophyllus |