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Literature summary for 2.3.1.140 extracted from

  • Petersen, M.; Alfermann, A.W.
    Two new enzymes of rosmarinic acid biosynthesis from cell cultures of Coleus blumei: Hydroxyphenylpyruvate reductase and rosmarinic acid synthase (1988), Z. Naturforsch. C, 43c, 501-504.
No PubMed abstract available
Search for more literature for 2.3.1.140

Activating Compound

Activating Compound Comment Organism Structure
ascorbate at 0.5 mM, 50% activation Coleus scutellarioides

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Coleus scutellarioides
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Coleus scutellarioides probably 4-coumaroyl-CoA and 4-hydroxyphenyllactate are the substrates used in vivo ?
-
 ?

Organism

Organism UniProt Comment Textmining
Coleus scutellarioides
-
 enzyme is induced by 4% sucrose content of the medium
-

Oxidation Stability

Oxidation Stability Organism
O2-sensitive, 10 mM DTT enhances enzyme activity 600%, 0.5 mM ascorbate enhances activity 200% Coleus scutellarioides

Purification (Commentary)

Purification (Comment) Organism
-
 Coleus scutellarioides

Storage Stability

Storage Stability Organism
-18°C, several weeks in desalted state Coleus scutellarioides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
caffeoyl-CoA + 3-(3,4-dihydroxyphenyl)lactate only R(+)-stereoisomer of 3-(3,4-dihydroxyphenyl)lactate is accepted Coleus scutellarioides CoA + rosmarinate
-
 ?
caffeoyl-CoA + 3-(4-hydroxyphenyl)lactate
-
 Coleus scutellarioides CoA + 2-O-caffeoyl-3-(4-hydroxyphenyl)lactate
-
 ?
additional information probably 4-coumaroyl-CoA and 4-hydroxyphenyllactate are the substrates used in vivo Coleus scutellarioides ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 5 h, no loss of activity Coleus scutellarioides