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Literature summary for 2.3.1.136 extracted from
- Acetyl-coenzyme A:polysialic acid O-acetyltransferase from K1-positive Escherichia coli. The enzyme responsible for the O-acetyl plus phenotype and for O-acetyl form variation (1988), J. Biol. Chem., 263, 8872-8878.
General Stability
| General Stability | Organism |
|---|---|
| endogenous polysialic acid stabilizes by a close non-covalent association with the enzyme | Escherichia coli |
| Triton X-100, 0.05%, stabilizes | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CoA | - |
Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
acetyl-CoA | - |
Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | eventually membrane-bound or membrane-associated protein | Escherichia coli | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement of a divalent cation | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K1 OAc+ substrains | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, at least 6 months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + colominic acid | substrate is a commercially available mixture of polysialic acid fragments, about 15 sialic acid residues per polymer, polysialic acids with more than 14 residues are acylated | Escherichia coli | CoA + O-(acetyl)-colominic acid | product is polysialic acid 7- or 9-O-acetylated | ? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable for 1 h | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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