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Literature summary for 2.3.1.135 extracted from

  • Jiang, W.; Napoli, J.L.
    Reorganization of cellular retinol-binding protein type 1 and lecithin:retinol acyltransferase during retinyl ester biosynthesis (2012), Biochim. Biophys. Acta, 1820, 859-869.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.135

Cloned(Commentary)

Cloned (Comment) Organism
stable expression of GFP-tagged LRAT in CHO cells and of GFP-tagged LRAT in COS7 cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum before initiation of retinyl ester biosynthesis, LRAT distributes throughout the endoplasmic reticulum, and Crpb1 localizes with mitochondria associated membranes, surrounded by LRAT. Upon initiating retinyl ester biosynthesis in cells, Crpb1 remains with MAM, and both Crbp1 and MAM re-localize with LRAT. LRAT formed rings around the growing lipid droplets Homo sapiens 5783
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lipid droplet upon initiating retinyl ester biosynthesis in cells, Crpb1 remains with MAM, and both Crbp1 and MAM re-localize with LRAT. LRAT formed rings around the growing lipid droplets. LRAT-containing rings colocalize with the lipid-droplet surface proteins, desnutrin/adipose triglyceride lipase and perilipin 2. Colocalization with lipid droplets requires the 38 N-terminal amino acid residues of LRAT, and specifically K36 and R38. Formation of rings around the growing lipid droplets does not require functional microtubules Homo sapiens 5811
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additional information subcellular localization study, overview Homo sapiens
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphatidylcholine + retinol-[cellular-retinol-binding-protein] Homo sapiens
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 2-acylglycerophosphocholine + retinyl-ester-[cellular-retinol-binding-protein]
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylcholine + retinol-[cellular-retinol-binding-protein]
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 Homo sapiens 2-acylglycerophosphocholine + retinyl-ester-[cellular-retinol-binding-protein]
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 ?

Synonyms

Synonyms Comment Organism
lecithin:retinol acyltransferase
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 Homo sapiens
LRAT
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 Homo sapiens

General Information

General Information Comment Organism
physiological function relationship between LRAT and Crbp1 during retinyl ester biosynthesis in which mitochondria associated membranes-associated Crpb1 and LRAT colocalize, and both surround the growing retinyl ester-containing lipid droplet. The N-terminus of LRAT, especially K36 and R38, is essential to colocalization with the lipid droplet Homo sapiens