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Literature summary for 2.3.1.135 extracted from

  • Bok, D.; Ruiz, A.; Yaron, O.; Jahng, W.J.; Ray, A.; Xue, L.; Rando, R.R.
    Purification and characterization of a transmembrane domain-deleted form of lecithin retinol acyltransferase (2003), Biochemistry, 42, 6090-6098.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.135

Cloned(Commentary)

Cloned (Comment) Organism
expression of a truncated enzyme form lacking the transmembrane N- and C-termini in Escherichia coli as His-tagged protein, which is insoluble in absence of detergents Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetics Homo sapiens
0.00024
-
 all-trans-retinol
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane integral Homo sapiens 16020
-
smooth endoplasmic reticulum
-
 Homo sapiens 5790
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 1 * 25000, full-length wild-type enzyme, SDS-PAGE, 1 * 20000, recombinant truncated enzyme, SDS-PAGE Homo sapiens
20000
-
 2 * 25000, full-length wild-type enzyme, SDS-PAGE, 2 * 20000, recombinant truncated enzyme, SDS-PAGE Homo sapiens
25000
-
 1 * 25000, full-length wild-type enzyme, SDS-PAGE, 1 * 20000, recombinant truncated enzyme, SDS-PAGE Homo sapiens
25000
-
 2 * 25000, full-length wild-type enzyme, SDS-PAGE, 2 * 20000, recombinant truncated enzyme, SDS-PAGE Homo sapiens
40000
-
 nonreducing PAGE, higher aggregation, up to pentamers, occurs in absence of denaturing agents Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant truncated enzyme from Escherichia coli in presence of 1% SDS, by nickel affinity chromatography to homogeneity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-
retina
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylcholine + 11-cis-retinol low activity Homo sapiens 2-acylglycerophosphocholine + 11-cis-retinyl acyl ester
-
 ?
phosphatidylcholine + all-trans-retinol
-
 Homo sapiens 2-acylglycerophosphocholine + all-trans-retinyl acylester
-
 ?
phosphatidylcholine + retinol-[cellular-retinol-binding-protein]
-
 Homo sapiens 2-acylglycerophosphocholine + retinyl-ester-[cellular-retinol-binding-protein]
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 25000, full-length wild-type enzyme, SDS-PAGE, 2 * 20000, recombinant truncated enzyme, SDS-PAGE Homo sapiens
monomer 1 * 25000, full-length wild-type enzyme, SDS-PAGE, 1 * 20000, recombinant truncated enzyme, SDS-PAGE Homo sapiens
More the enzyme exists as a mixture of monomer and dimer, determined by sedimentation equilibrium analysis and mass spectrometry, higher aggregation, up to pentamers, occurs in absence of denaturing agents Homo sapiens

Synonyms

Synonyms Comment Organism
lecithin retinol acyltransferase
-
 Homo sapiens
LRAT
-
 Homo sapiens