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Literature summary for 2.3.1.135 extracted from

  • Ruiz, A.; Winston, A.; Lim, Y.H.; Gilbert, B.A.; Rando, R.R.; Bok, D.
    Molecular and biochemical characterization of lecithin retinol acyltransferase (1999), J. Biol. Chem., 274, 3834-3841.
    View publication on PubMed
Search for more literature for 2.3.1.135

Cloned(Commentary)

Cloned (Comment) Organism
expression in HEK-293 cells Homo sapiens
expression in HEK-293T cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
all-trans-retinyl-alpha-bromoacetate
-
 Bos taurus
N-Boc-L-biocytinyl-11-aminoundecan chloromethylketone 0.002 mM, 45% inhibition; irreversible inhibitor; time dependent inhibition Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphatidylcholine + all-trans-retinol Bos taurus
-
 all-trans-retinyl acyl esters + 2-acylglycerophosphocholine
-
 ?
phosphatidylcholine + all-trans-retinol Homo sapiens
-
 all-trans-retinyl acyl esters + 2-acylglycerophosphocholine
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Homo sapiens O95237
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity labeling with all-trans-retinyl-alpha-bromoacetate, SDS electrophoresis, prior to affinity labelling, incubation with cholesterol chloroacetate to block nucleophilic reagents, or streptavidin column to remove endogenously biotinylated proteins enhance protein purification Bos taurus
affinity labeling with N-Boc-L-biocytinyl-11-aminoundecan chloromethylketone, precipitation and gel electrophoresis, prior to affinity labelling, incubation with cholesterol chloroacetate to block nucleophilic reagents, or streptavidin column to remove endogenously biotinylated proteins enhance protein purification Bos taurus
labeling with N-Boc-L-biocytinyl-11-aminoundecan chloromethylketone, affinity chromatography, avidin, prior to affinity chromatography or affinity labelling, incubation with cholesterol chloroacetate to block nucleophilic reagents, or streptavidin column to remove endogenously biotinylated proteins enhance protein purification Bos taurus
solubilization of membranes and centrifugation, chromatography on DEAE-column and Mono-Q column later on, quantitative information Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver mRNA expression in adult and fetal stage Homo sapiens
-
pancreas mRNA expression in adult stage Homo sapiens
-
retinal pigment epithelium
-
 Bos taurus
-
retinal pigment epithelium
-
 Homo sapiens
-
small intestine mRNA expression Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dipalmitoylphosphatidylcholine + all-trans-retinol
-
 Bos taurus all-trans-retinyl palmitate + 2-palmitoylglycerophosphocholine
-
 r
dipalmitoylphosphatidylcholine + all-trans-retinol mechanistic hypothesis Homo sapiens all-trans-retinyl palmitate + 2-palmitoylglycerophosphocholine
-
 r
additional information
-
 Bos taurus ?
-
 ?
additional information only fatty acyl group at the sn-1 is transferred Homo sapiens ?
-
 ?
phosphatidylcholine + all-trans-retinol
-
 Bos taurus all-trans-retinyl acyl esters + 2-acylglycerophosphocholine
-
 ?
phosphatidylcholine + all-trans-retinol
-
 Homo sapiens all-trans-retinyl acyl esters + 2-acylglycerophosphocholine
-
 ?
phosphatidylcholine + all-trans-retinol-(bovine serum albumin)
-
 Homo sapiens all-trans-retinyl acyl ester-(bovine serum albumin) + 2-acylglycerophosphocholine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 × 25300, SDS-PAGE in presence of 2-mercaptoethanol, fully active catalytically Bos taurus
monomer 1 × 25000, fully active catalytically Homo sapiens