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Literature summary for 2.3.1.135 extracted from
- Acyl-CoA:retinol acyltransferase and lecithin:retinol acyltransferase activities of bovine retinal pigment epithelial microsomes (1990), Methods Enzymol., 190, 156-163.
General Stability
| General Stability | Organism |
|---|---|
| all operation involving retinoids are performed at darkness, or under red light | Rattus norvegicus |
| all operation involving retinoids are performed at darkness, or under red light | Bos taurus |
| buffers are bubbled with argon prior to use | Bos taurus |
| dithiothreitol stabilizes | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
all-trans-retinol | - |
Bos taurus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Rattus norvegicus | - |
- |
| microsome | - |
Bos taurus | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| intestinal mucosa | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| mammary gland | - |
Rattus norvegicus | - |
| retinal pigment epithelium | - |
Bos taurus | - |
| testis | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00003 | - |
intestinal LRAT | Rattus norvegicus |
| 0.00006 | - |
mammary gland LRAT | Rattus norvegicus |
| 0.000094 | - |
testis LRAT | Rattus norvegicus |
| 0.000145 | - |
liver LRAT | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, 10 mM Tris acetate, pH 7, 1 mM dithiothreitol, microsomal fraction, stable for at least 6 months | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | little or no ability to transfer acyl groups from lysophosphatidylcholine, phosphatidylethanolamine or phosphatidic acid to retinol-(cellular-retinol-binding protein) | Rattus norvegicus | ? | - |
? | |
| additional information | the fatty acid in the 2-position is important in substrate recognition | Rattus norvegicus | ? | - |
? | |
| additional information | the fatty acid in the 2-position is important in substrate recognition | Bos taurus | ? | - |
? | |
| phosphatidylcholine + retinol-(cellular-retinol-binding-protein) | - |
Rattus norvegicus | 2-acylglycerophosphocholine + retinyl ester-(cellular-retinol-binding-protein) | - |
? | |
| phosphatidylcholine + retinol-(cellular-retinol-binding-protein) | - |
Bos taurus | 2-acylglycerophosphocholine + retinyl ester-(cellular-retinol-binding-protein) | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Rattus norvegicus |
| 37 | - |
assay at | Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
half-life in absence of substrate: 17 min | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | - |
Bos taurus |
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Release 2023.1 (January 2023)
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