Cloned (Comment) | Organism |
---|---|
- |
Sorghum bicolor |
Crystallization (Comment) | Organism |
---|---|
structures in its apo-form and ternary complex with shikimate and 4-coumaroyl-CoA, which is converted to its product during crystal soaking. Residues threonine36, serine38, tyrosine40, histidine162, arginine371, and threonine384 are involved in catalysis and specificity. Histidine162 and threonine36 play a role in the catalytic mechanism. Substrate binding should occur sequentially, with 4-coumaroyl-CoA binding prior to the acyl acceptor molecule. Comparison of the structure of sorghum HCT with the HCT involved in chlorogenic acid synthesis in Coffea canephora | Sorghum bicolor |
Protein Variants | Comment | Organism |
---|---|---|
H162A | complete loss of activity | Sorghum bicolor |
R371A | complete loss of activity | Sorghum bicolor |
S38A | 40% of wild-type activity | Sorghum bicolor |
T36A | 6% of wild-type activity | Sorghum bicolor |
T384A | 9% of wild-type activity | Sorghum bicolor |
Y40A | 62% of wild-type activity | Sorghum bicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0631 | - |
shikimate | mutant Y40A, pH 6.5, temperature not specified in the publication | Sorghum bicolor | |
0.153 | - |
shikimate | wild-type, pH 6.5, temperature not specified in the publication | Sorghum bicolor | |
0.678 | - |
shikimate | mutant S38A, pH 6.5, temperature not specified in the publication | Sorghum bicolor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
static light scattering | Sorghum bicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sorghum bicolor | C5XXB7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + quinate | low activity towards quinate | Sorghum bicolor | CoA + 5-O-(4-coumaroyl)quinate | - |
? | |
4-coumaroyl-CoA + shikimate | - |
Sorghum bicolor | CoA + 5-O-(4-coumaroyl)shikimate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 49000, calculated | Sorghum bicolor |