Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.133 extracted from

  • Walker, A.M.; Hayes, R.P.; Youn, B.; Vermerris, W.; Sattler, S.E.; Kang, C.
    Elucidation of the structure and reaction mechanism of sorghum hydroxycinnamoyltransferase and its structural relationship to other coenzyme a-dependent transferases and synthases (2013), Plant Physiol., 162, 640-651.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Sorghum bicolor

Crystallization (Commentary)

Crystallization (Comment) Organism
structures in its apo-form and ternary complex with shikimate and 4-coumaroyl-CoA, which is converted to its product during crystal soaking. Residues threonine36, serine38, tyrosine40, histidine162, arginine371, and threonine384 are involved in catalysis and specificity. Histidine162 and threonine36 play a role in the catalytic mechanism. Substrate binding should occur sequentially, with 4-coumaroyl-CoA binding prior to the acyl acceptor molecule. Comparison of the structure of sorghum HCT with the HCT involved in chlorogenic acid synthesis in Coffea canephora Sorghum bicolor

Protein Variants

Protein Variants Comment Organism
H162A complete loss of activity Sorghum bicolor
R371A complete loss of activity Sorghum bicolor
S38A 40% of wild-type activity Sorghum bicolor
T36A 6% of wild-type activity Sorghum bicolor
T384A 9% of wild-type activity Sorghum bicolor
Y40A 62% of wild-type activity Sorghum bicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0631
-
shikimate mutant Y40A, pH 6.5, temperature not specified in the publication Sorghum bicolor
0.153
-
shikimate wild-type, pH 6.5, temperature not specified in the publication Sorghum bicolor
0.678
-
shikimate mutant S38A, pH 6.5, temperature not specified in the publication Sorghum bicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
static light scattering Sorghum bicolor

Organism

Organism UniProt Comment Textmining
Sorghum bicolor C5XXB7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-coumaroyl-CoA + quinate low activity towards quinate Sorghum bicolor CoA + 5-O-(4-coumaroyl)quinate
-
?
4-coumaroyl-CoA + shikimate
-
Sorghum bicolor CoA + 5-O-(4-coumaroyl)shikimate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 49000, calculated Sorghum bicolor