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Literature summary for 2.3.1.133 extracted from
- Hydroxycinnamoyltransferases Involved in the accumulation of caffeic acid esters in gametophytes and sporophytes of Equisetum arvense (1996), Plant Physiol., 111, 1153-1159.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.41 | - |
caffeoyl-CoA | - |
Equisetum arvense |  |
| 0.5 | - |
4-coumaroyl-CoA | - |
Equisetum arvense | |
| 1.1 | - |
3-phenylacryloyl-CoA | - |
Equisetum arvense | |
| 1.9 | - |
feruloyl-CoA | - |
Equisetum arvense | |
| 4.32 | - |
shikimate | - |
Equisetum arvense | |
| 5.5 | - |
shikimate | - |
Equisetum arvense | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
gel filtration | Equisetum arvense |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Equisetum arvense | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Equisetum arvense |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
change of enzyme activity during a 1-year growth period | Equisetum arvense |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, for several months with 10-20% loss of apparent activities after thawing | Equisetum arvense |
| -80°C, for several months with 10-20% loss of apparent activities after thawing | Equisetum arvense |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-phenylacryloyl-CoA + shikimate | - |
Equisetum arvense | CoA + 3-phenylacryloylshikimate | - |
? | |
| 4-coumaroyl-CoA + shikimate | shikimate cannot be replaced by quinate | Equisetum arvense | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
| caffeoyl-CoA + shikimate | - |
Equisetum arvense | CoA + 5-O-caffeoylshikimate | - |
? | |
| cinnamoyl-CoA + shikimate | - |
Equisetum arvense | CoA + 5-O-cinnamoylshikimate | - |
? | |
| feruloyl-CoA + shikimate | - |
Equisetum arvense | CoA + 5-O-feruloylshikimate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Equisetum arvense |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
Tris-HCl | Equisetum arvense |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 8.5 | about half-maximal activity at pH 6.2 in potassium phosphate buffer and 8.5 in Tris-HCl puffer | Equisetum arvense |
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Release 2023.1 (January 2023)
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