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Literature summary for 2.3.1.132 extracted from

  • Strack, D.; Keller, H.; Weissenböck, G.
    Enzymatic synthesis of hydroxycinnamic acid esters of sugar acids and hydroaromatic acids by protein preparations from rye (Secale cereale) primary leaves (1987), J. Plant Physiol., 131, 61-73.
No PubMed abstract available
Search for more literature for 2.3.1.132

Activating Compound

Activating Compound Comment Organism Structure
DTT activates Secale cereale

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
 sinapoyl-CoA
-
 Secale cereale
10
-
 glucarolactone
-
 Secale cereale

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sinapoyl-CoA + glucarolactone Secale cereale best substrate CoA + O-sinapoylglucarolactone
-
 ?

Organism

Organism UniProt Comment Textmining
Secale cereale
-
 rye, var. Kustro
-

Purification (Commentary)

Purification (Comment) Organism
partial Secale cereale

Source Tissue

Source Tissue Comment Organism Textmining
primary leaf
-
 Secale cereale
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.022
-
-
 Secale cereale

Storage Stability

Storage Stability Organism
-20°C, at least 6 months Secale cereale

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
caffeoyl-CoA + glucarolactone poor substrate Secale cereale CoA + O-caffeoylglucarolactone
-
 ?
feruloyl-CoA + glucarolactone condensation at 37% the rate of the reaction with sinapoyl-CoA Secale cereale CoA + O-feruloylglucarolactone
-
 ?
additional information no activity with 1-hydroxycinnamoyl glucose esters, gluconolactone, galactonate, glucuronate, ascorbate, malate, m-tartrate Secale cereale ?
-
 ?
p-coumaroyl-CoA + glucarolactone condensation at 54% the rate of the reaction with sinapoyl-CoA Secale cereale CoA + O-p-coumaroylglucarolactone
-
 ?
sinapoyl-CoA + glucarolactone best substrate Secale cereale CoA + O-sinapoylglucarolactone
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 denaturation after 5 min Secale cereale

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 50 mM potassium phosphate buffer Secale cereale

pH Range

pH Minimum pH Maximum Comment Organism
7.4 8.2 about 80% of maximal activity at pH 7.4 and pH 8.2 Secale cereale