Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | activation | Secale cereale |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
sinapoyl-CoA | - |
Secale cereale | |
8 | - |
glucarate | - |
Secale cereale |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Secale cereale | - |
rye, var. Kustro | - |
Purification (Comment) | Organism |
---|---|
partial | Secale cereale |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | primary | Secale cereale | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01 | - |
feruloyl-CoA as donor | Secale cereale |
Storage Stability | Organism |
---|---|
-20°C, at least 6 months | Secale cereale |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
caffeoyl-CoA + glucarate | condensation at 8% the rate of the reaction with sinapoyl-CoA | Secale cereale | CoA + caffeoylglucarate | - |
? | |
feruloyl-CoA + glucarate | condensation at 54% the rate of the reaction with sinapoyl-CoA | Secale cereale | CoA + feruloylglucarate | - |
? | |
additional information | no substrates are 1-hydroxycinnamoyl glucose esters, gluconolactone, galactonate, glucuronate, ascorbate, malate, m-tartrate | Secale cereale | ? | - |
? | |
p-coumaroyl-CoA + glucarate | condensation at 85% the rate of the reaction with sinapoyl-CoA | Secale cereale | CoA + p-coumaroylglucarate | - |
? | |
sinapoyl-CoA + glucarate | best substrate | Secale cereale | CoA + O-sinapoylglucarate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Secale cereale |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
denaturation after 5 min | Secale cereale |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
100 mM potassium phosphate buffer | Secale cereale |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 8 | about 80% of maximal activity at pH 6.2 and 8.0 | Secale cereale |