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Literature summary for 2.3.1.131 extracted from

  • Strack, D.; Keller, H.; Weissenböck, G.
    Enzymatic synthesis of hydroxycinnamic acid esters of sugar acids and hydroaromatic acids by protein preparations from rye (Secale cereale) primary leaves (1987), J. Plant Physiol., 131, 61-73.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
EDTA activation Secale cereale

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
sinapoyl-CoA
-
Secale cereale
8
-
glucarate
-
Secale cereale

Organism

Organism UniProt Comment Textmining
Secale cereale
-
rye, var. Kustro
-

Purification (Commentary)

Purification (Comment) Organism
partial Secale cereale

Source Tissue

Source Tissue Comment Organism Textmining
leaf primary Secale cereale
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
feruloyl-CoA as donor Secale cereale

Storage Stability

Storage Stability Organism
-20°C, at least 6 months Secale cereale

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
caffeoyl-CoA + glucarate condensation at 8% the rate of the reaction with sinapoyl-CoA Secale cereale CoA + caffeoylglucarate
-
?
feruloyl-CoA + glucarate condensation at 54% the rate of the reaction with sinapoyl-CoA Secale cereale CoA + feruloylglucarate
-
?
additional information no substrates are 1-hydroxycinnamoyl glucose esters, gluconolactone, galactonate, glucuronate, ascorbate, malate, m-tartrate Secale cereale ?
-
?
p-coumaroyl-CoA + glucarate condensation at 85% the rate of the reaction with sinapoyl-CoA Secale cereale CoA + p-coumaroylglucarate
-
?
sinapoyl-CoA + glucarate best substrate Secale cereale CoA + O-sinapoylglucarate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Secale cereale

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
denaturation after 5 min Secale cereale

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
100 mM potassium phosphate buffer Secale cereale

pH Range

pH Minimum pH Maximum Comment Organism
6.2 8 about 80% of maximal activity at pH 6.2 and 8.0 Secale cereale