Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mus musculus |
expression in Escherichia coli | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ethanolamine | about 37% inhibition at 100 mM | Mus musculus | |
Gly-Gly-Gly | about 37% inhibition at 100 mM | Mus musculus | |
glycolic acid | - |
Mus musculus | |
additional information | not inhibited by alanine, beta-alanine, valine, and threonine | Mus musculus | |
oleoyl-CoA | - |
Mus musculus | |
serine | about 15% inhibition at 100 mM | Mus musculus | |
tyramine | about 53% inhibition at 100 mM | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0094 | - |
benzoyl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
0.0094 | - |
benzoyl-CoA | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
0.045 | - |
hexanoyl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
0.052 | - |
butyryl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
0.052 | - |
butyryl-CoA | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
0.209 | - |
acetyl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
0.209 | - |
acetyl-CoA | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
6.1 | - |
glycine | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
9.7 | - |
glycine ethyl ester | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
29 | - |
Glycine methyl ester | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
450 | - |
hexanoyl-CoA | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
x * 34000, SDS-PAGE | Mus musculus |
34000 | - |
x * 34000, calculated | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q91XE0 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography, and gel filtration | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + glycine | - |
Mus musculus | CoA + N-acetylglycine | - |
? | |
benzoyl-CoA + glycine | best substrate combination | Mus musculus | CoA + N-benzoylglycine | i.e. hippurate | ? | |
benzoyl-CoA + glycine | glycine serves as the best amino donor substrate, and benzoyl-CoA serves as the best the amino acceptor substrate | Mus musculus | CoA + N-benzoylglycine | - |
? | |
benzoyl-CoA + glycine ethyl ester | - |
Mus musculus | CoA + N-benzoylglycine ethyl ester | - |
? | |
benzoyl-CoA + glycine methyl ester | - |
Mus musculus | CoA + N-benzoylglycine methyl ester | - |
? | |
butyryl-CoA + glycine | - |
Mus musculus | CoA + N-butyrylglycine | - |
? | |
hexanoyl-CoA + glycine | - |
Mus musculus | CoA + N-hexanoylglycine | - |
? | |
additional information | enzyme also shows glycine N-benzoyltransferase activity, reaction of EC 2.3.1.71. Amino acceptor substrate preference in decreasing order is benzoyl-CoA, butyryl-CoA, hexanoyl-CoA, acetyl-CoA | Mus musculus | ? | - |
? | |
additional information | no activity with oleoyl-CoA | Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, SDS-PAGE | Mus musculus |
? | x * 34000, calculated | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
GlyAT | - |
Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.57 | - |
glycine ethyl ester | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
0.8 | - |
acetyl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
0.8 | - |
acetyl-CoA | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
1 | - |
Glycine methyl ester | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
3.2 | - |
glycine | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
4.2 | - |
benzoyl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
4.2 | - |
benzoyl-CoA | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
4.6 | - |
hexanoyl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
4.6 | - |
hexanoyl-CoA | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
15 | - |
butyryl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
15 | - |
butyryl-CoA | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.021 | - |
at pH 8.0, temperature not specified in the publication | Mus musculus | oleoyl-CoA | |
58 | - |
apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | glycolic acid |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
Glycine methyl ester | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
0.059 | - |
glycine ethyl ester | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
0.52 | - |
glycine | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
3.8 | - |
acetyl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
3.8 | - |
acetyl-CoA | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
100 | - |
hexanoyl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
100 | - |
hexanoyl-CoA | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
290 | - |
butyryl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
290 | - |
butyryl-CoA | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus | |
450 | - |
benzoyl-CoA | pH 8.0, temperature not specified in the publication | Mus musculus | |
450 | - |
benzoyl-CoA | apparent value, at pH 8.0, temperature not specified in the publication | Mus musculus |