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Literature summary for 2.3.1.13 extracted from

  • Dempsey, D.R.; Bond, J.D.; Carpenter, A.M.; Rodriguez Ospina, S.; Merkler, D.J.
    Expression, purification, and characterization of mouse glycine N-acyltransferase in Escherichia coli (2014), Protein Expr. Purif., 97, 23-28.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mus musculus
expression in Escherichia coli Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
ethanolamine about 37% inhibition at 100 mM Mus musculus
Gly-Gly-Gly about 37% inhibition at 100 mM Mus musculus
glycolic acid
-
Mus musculus
additional information not inhibited by alanine, beta-alanine, valine, and threonine Mus musculus
oleoyl-CoA
-
Mus musculus
serine about 15% inhibition at 100 mM Mus musculus
tyramine about 53% inhibition at 100 mM Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0094
-
benzoyl-CoA pH 8.0, temperature not specified in the publication Mus musculus
0.0094
-
benzoyl-CoA apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
0.045
-
hexanoyl-CoA pH 8.0, temperature not specified in the publication Mus musculus
0.052
-
butyryl-CoA pH 8.0, temperature not specified in the publication Mus musculus
0.052
-
butyryl-CoA apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
0.209
-
acetyl-CoA pH 8.0, temperature not specified in the publication Mus musculus
0.209
-
acetyl-CoA apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
6.1
-
glycine apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
9.7
-
glycine ethyl ester apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
29
-
Glycine methyl ester apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
450
-
hexanoyl-CoA apparent value, at pH 8.0, temperature not specified in the publication Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
x * 34000, SDS-PAGE Mus musculus
34000
-
x * 34000, calculated Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q91XE0
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography, and gel filtration Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + glycine
-
Mus musculus CoA + N-acetylglycine
-
?
benzoyl-CoA + glycine best substrate combination Mus musculus CoA + N-benzoylglycine i.e. hippurate ?
benzoyl-CoA + glycine glycine serves as the best amino donor substrate, and benzoyl-CoA serves as the best the amino acceptor substrate Mus musculus CoA + N-benzoylglycine
-
?
benzoyl-CoA + glycine ethyl ester
-
Mus musculus CoA + N-benzoylglycine ethyl ester
-
?
benzoyl-CoA + glycine methyl ester
-
Mus musculus CoA + N-benzoylglycine methyl ester
-
?
butyryl-CoA + glycine
-
Mus musculus CoA + N-butyrylglycine
-
?
hexanoyl-CoA + glycine
-
Mus musculus CoA + N-hexanoylglycine
-
?
additional information enzyme also shows glycine N-benzoyltransferase activity, reaction of EC 2.3.1.71. Amino acceptor substrate preference in decreasing order is benzoyl-CoA, butyryl-CoA, hexanoyl-CoA, acetyl-CoA Mus musculus ?
-
?
additional information no activity with oleoyl-CoA Mus musculus ?
-
?

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Mus musculus
? x * 34000, calculated Mus musculus

Synonyms

Synonyms Comment Organism
GlyAT
-
Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.57
-
glycine ethyl ester apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
0.8
-
acetyl-CoA pH 8.0, temperature not specified in the publication Mus musculus
0.8
-
acetyl-CoA apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
1
-
Glycine methyl ester apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
3.2
-
glycine apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
4.2
-
benzoyl-CoA pH 8.0, temperature not specified in the publication Mus musculus
4.2
-
benzoyl-CoA apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
4.6
-
hexanoyl-CoA pH 8.0, temperature not specified in the publication Mus musculus
4.6
-
hexanoyl-CoA apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
15
-
butyryl-CoA pH 8.0, temperature not specified in the publication Mus musculus
15
-
butyryl-CoA apparent value, at pH 8.0, temperature not specified in the publication Mus musculus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.021
-
at pH 8.0, temperature not specified in the publication Mus musculus oleoyl-CoA
58
-
apparent value, at pH 8.0, temperature not specified in the publication Mus musculus glycolic acid

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.035
-
Glycine methyl ester apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
0.059
-
glycine ethyl ester apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
0.52
-
glycine apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
3.8
-
acetyl-CoA pH 8.0, temperature not specified in the publication Mus musculus
3.8
-
acetyl-CoA apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
100
-
hexanoyl-CoA pH 8.0, temperature not specified in the publication Mus musculus
100
-
hexanoyl-CoA apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
290
-
butyryl-CoA pH 8.0, temperature not specified in the publication Mus musculus
290
-
butyryl-CoA apparent value, at pH 8.0, temperature not specified in the publication Mus musculus
450
-
benzoyl-CoA pH 8.0, temperature not specified in the publication Mus musculus
450
-
benzoyl-CoA apparent value, at pH 8.0, temperature not specified in the publication Mus musculus