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Literature summary for 2.3.1.13 extracted from

  • Kelley, M.; Vessey, D.A.
    Isolation and characterization of mitochondrial acyl-CoA: glycine N-acyltransferase from kidney (1993), J. Biochem. Toxicol., 8, 65-69.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
KCl 100 mM, 50% inhibition Bos taurus
N-benzoylglycine
-
Bos taurus
p-chloromercuribenzoate 1 mM, 90% inhibition after 40 min, 20% activity can be recovered by adding 20fold excess of dithiothreitol, benzoyl-CoA protects Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6
-
glycine acyl donor benzoyl-CoA Bos taurus
7.6
-
glycine acyl donor salicyl-CoA Bos taurus
79
-
glycine acyl donor butyryl-CoA Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33500
-
gel filtration Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DE-52, gel filtration, chromatofocusing Bos taurus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
10
-
acyl donor benzoyl-CoA Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + glycine
-
Bos taurus CoA + N-acylglycine
-
?
benzoyl-CoA + glycine
-
Bos taurus CoA + N-benzoylglycine
-
?
butyryl-CoA + glycine 19% of activity with benzoyl-coA Bos taurus CoA + N-butyrylglycine
-
?
heptanoyl-CoA + glycine 3.9% of activity with benzoyl-coA Bos taurus CoA + N-heptanoylglycine
-
?
isovaleryl-CoA + glycine 8.4% of activity with benzoyl-coA Bos taurus CoA + N-isovalerylglycine
-
?
salicyl-CoA + glycine 15% of activity with benzoyl-coA Bos taurus CoA + salicyluric acid
-
?

Subunits

Subunits Comment Organism
monomer 1 * 33500, SDS-PAGE Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.075
-
N-benzoylglycine
-
Bos taurus