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Literature summary for 2.3.1.13 extracted from

  • Nandi, D.L.; Lucas, S.V.; Webster, L.T.
    Benzoyl-coenzyme A:glycine N-acyltransferase and phenylacetyl-coenzyme A:glycine N-acyltransferase from bovine liver mitochondria. Purification and characterization (1979), J. Biol. Chem., 254, 7230-7237.
    View publication on PubMed

General Stability

General Stability Organism
enzyme fractions from hydroxylapatite column are inactivated by dialysis or concentration by ultracentrifugation Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate)
-
Bos taurus
benzoyl-CoA
-
Bos taurus
butyryl-CoA
-
Bos taurus
Mg2+
-
Bos taurus
Ni2+
-
Bos taurus
p-chloromercuribenzoate
-
Bos taurus
Zn2+
-
Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Bos taurus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cs+ K+, Rb+, Na+, Li+, Cs+ or NH4+ required for activity Bos taurus
K+ K+, Rb+, Na+, Li+, Cs+ or NH4+ required for activity Bos taurus
Li+ K+, Rb+, Na+, Li+, Cs+ or NH4+ required for activity Bos taurus
Na+ K+, Rb+, Na+, Li+, Cs+ or NH4+ required for activity Bos taurus
NH4+ K+, Rb+, Na+, Li+, Cs+ or NH4+ required for activity Bos taurus
Rb+ K+, Rb+, Na+, Li+, Cs+ or NH4+ required for activity Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
SDS-PAGE, sucrose density gradient centrifugation Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Reaction

Reaction Comment Organism Reaction ID
acyl-CoA + glycine = CoA + N-acylglycine sequential reaction mechanism, acyl-CoA substrate adds to the enzyme first, glycine adds before CoA leaves and the peptide product dissociates last Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Bos taurus
-

Storage Stability

Storage Stability Organism
4Ā°C, 1-3 weeks, about 25% loss of activity Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + glycine phenylacetyl-CoA or indoleacetyl-CoA can not act as acyl donor Bos taurus CoA + N-acylglycine
-
?
acyl-CoA + L-asparagine
-
Bos taurus ?
-
?
acyl-CoA + L-glutamine
-
Bos taurus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 33000, SDS-PAGE Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4 8.6
-
Bos taurus