Cloned (Comment) | Organism |
---|---|
gene lpxA, excluding the region encoding the predicted signal peptide (residues 2-32), expression of the plant enzyme in Escherichia coli strain C41(DE3). Functional expression of AtlpxA in and complementation of the constructed lpxA Escherichia coli knockout mutant strains | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
purified recombinant LpxA, sitting drop vapor diffusion method, mixing of AtLpxA at 18 mg/mL in 10 mM potassium phosphate buffer, pH 7.0, 200 mM KCl, and 20% glycerol, in a 1:1 ratio with precipitant solution containing 0.5 M ammonium sulfate, 0.1 M sodium citrate, pH 5.6, and 1.0 M lithium sulfate monohydrate, equilibration at 20°C, 1-2 days, X-ray diffraction structure determination and analysis at 2.1 A resolution | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | Arabidopsis thaliana lpxA complements an Escherichia coli mutant lacking the chromosomal lpxA and promotes the synthesis of lipid A in vivo similar to the lipid A produced in the presence of Escherichia coli lpxA | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
an (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine | Arabidopsis thaliana | - |
an [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9SU91 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant LpxA, excluding the region encoding the predicted signal peptide (residues 2-32), from Escherichia coli strain C41(DE3) by Reactive Green 19 affinity chromatography and gel filtration to homogeneity | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
an (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine | - |
Arabidopsis thaliana | an [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine | - |
r | |
an (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine | specificity for (R)-3-hydroxymyristoyl-ACP over other chain lengths in vitro | Arabidopsis thaliana | an [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
LpxA | - |
Arabidopsis thaliana |
UDP-N-acetylglucosamine acyltransferase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | the overall structure of AtLpxA is very similar to that of Escherichia coli LpxA, EcLpxA, with an alpha-helical-rich C-terminus and characteristic N-terminal left-handed parallel beta-helix. All key catalytic and chain length determining residues of EcLpxA are conserved in AtLpxA,but AtLpxA has an additional coil and loop added to the N-terminal left-handed parallel beta-helix not seen in EcLpxA | Arabidopsis thaliana |
additional information | active site structure of AtLpxA, overview | Arabidopsis thaliana |