BRENDA - Enzyme Database
show all sequences of 2.3.1.126

Hydroxycinnamic acid esters of isocitric acid: Accumulation and enzymatic synthesis in Amaranthus cruentus

Strack, D.; Leicht, P.; Bokern, M.; Wray, V.; Grotjahn, L.; Phytochemistry 26, 2919-2923 (1987)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.045
-
caffeoyl-CoA
-
Amaranthus hybridus subsp. cruentus
0.055
-
p-Coumaroyl-CoA
-
Amaranthus hybridus subsp. cruentus
0.163
-
feruloyl-CoA
-
Amaranthus hybridus subsp. cruentus
0.208
-
isocitric acid
second substrate caffeoyl-CoA
Amaranthus hybridus subsp. cruentus
0.257
-
isocitric acid
second substrate p-coumaroyl-CoA
Amaranthus hybridus subsp. cruentus
0.412
-
isocitric acid
second substrate feruloyl-CoA
Amaranthus hybridus subsp. cruentus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
caffeoyl-CoA + isocitrate
Amaranthus hybridus
-
CoA + 2-caffeoylisocitrate
-
-
r
caffeoyl-CoA + isocitrate
Amaranthus tricolor
-
CoA + 2-caffeoylisocitrate
-
-
r
caffeoyl-CoA + isocitrate
Amaranthus hybridus subsp. cruentus
-
CoA + 2-caffeoylisocitrate
-
-
r
caffeoyl-CoA + isocitrate
Amaranthus paniculatus
-
CoA + 2-caffeoylisocitrate
-
-
r
caffeoyl-CoA + isocitrate
Amaranthus paniculatus L.
-
CoA + 2-caffeoylisocitrate
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Amaranthus hybridus
-
ssp. paniculatus (L.) Hejny
-
Amaranthus hybridus subsp. cruentus
-
L. cv. Oeschberg; Willd.
-
Amaranthus paniculatus
-
L.
-
Amaranthus paniculatus L.
-
L.
-
Amaranthus tricolor
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Amaranthus hybridus subsp. cruentus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cotyledon
-
Amaranthus hybridus subsp. cruentus
-
leaf
-
Amaranthus hybridus subsp. cruentus
-
seedling
-
Amaranthus hybridus subsp. cruentus
-
Storage Stability
Storage Stability
Organism
-20°C, protein preparation shows no loss of enzymatic activity when stored for several weeks
Amaranthus hybridus subsp. cruentus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
caffeoyl-CoA + isocitrate
-
486247
Amaranthus hybridus
CoA + 2-caffeoylisocitrate
-
-
-
r
caffeoyl-CoA + isocitrate
-
486247
Amaranthus tricolor
CoA + 2-caffeoylisocitrate
-
-
-
r
caffeoyl-CoA + isocitrate
-
486247
Amaranthus hybridus subsp. cruentus
CoA + 2-caffeoylisocitrate
-
-
-
r
caffeoyl-CoA + isocitrate
-
486247
Amaranthus paniculatus
CoA + 2-caffeoylisocitrate
-
-
-
r
caffeoyl-CoA + isocitrate
-
486247
Amaranthus paniculatus L.
CoA + 2-caffeoylisocitrate
-
-
-
r
caffeoyl-CoA + isocitric acid
-
486247
Amaranthus hybridus subsp. cruentus
CoA + (E)-caffeoylisocitric acid
-
486247
Amaranthus hybridus subsp. cruentus
r
feruloyl-CoA + isocitric acid
-
486247
Amaranthus hybridus subsp. cruentus
CoA + feruloylisocitric acid
-
486247
Amaranthus hybridus subsp. cruentus
r
additional information
citric, malic tartaric and quinic acids are not accepted as substrates
486247
Amaranthus hybridus subsp. cruentus
?
-
-
-
-
sinapoyl-CoA + isocitric acid
minor activity compared to caffeoyl-CoA
486247
Amaranthus hybridus subsp. cruentus
CoA + sinapoylisocitric acid
-
486247
Amaranthus hybridus subsp. cruentus
r
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.045
-
caffeoyl-CoA
-
Amaranthus hybridus subsp. cruentus
0.055
-
p-Coumaroyl-CoA
-
Amaranthus hybridus subsp. cruentus
0.163
-
feruloyl-CoA
-
Amaranthus hybridus subsp. cruentus
0.208
-
isocitric acid
second substrate caffeoyl-CoA
Amaranthus hybridus subsp. cruentus
0.257
-
isocitric acid
second substrate p-coumaroyl-CoA
Amaranthus hybridus subsp. cruentus
0.412
-
isocitric acid
second substrate feruloyl-CoA
Amaranthus hybridus subsp. cruentus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
caffeoyl-CoA + isocitrate
Amaranthus hybridus
-
CoA + 2-caffeoylisocitrate
-
-
r
caffeoyl-CoA + isocitrate
Amaranthus tricolor
-
CoA + 2-caffeoylisocitrate
-
-
r
caffeoyl-CoA + isocitrate
Amaranthus hybridus subsp. cruentus
-
CoA + 2-caffeoylisocitrate
-
-
r
caffeoyl-CoA + isocitrate
Amaranthus paniculatus
-
CoA + 2-caffeoylisocitrate
-
-
r
caffeoyl-CoA + isocitrate
Amaranthus paniculatus L.
-
CoA + 2-caffeoylisocitrate
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Amaranthus hybridus subsp. cruentus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cotyledon
-
Amaranthus hybridus subsp. cruentus
-
leaf
-
Amaranthus hybridus subsp. cruentus
-
seedling
-
Amaranthus hybridus subsp. cruentus
-
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, protein preparation shows no loss of enzymatic activity when stored for several weeks
Amaranthus hybridus subsp. cruentus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
caffeoyl-CoA + isocitrate
-
486247
Amaranthus hybridus
CoA + 2-caffeoylisocitrate
-
-
-
r
caffeoyl-CoA + isocitrate
-
486247
Amaranthus tricolor
CoA + 2-caffeoylisocitrate
-
-
-
r
caffeoyl-CoA + isocitrate
-
486247
Amaranthus hybridus subsp. cruentus
CoA + 2-caffeoylisocitrate
-
-
-
r
caffeoyl-CoA + isocitrate
-
486247
Amaranthus paniculatus
CoA + 2-caffeoylisocitrate
-
-
-
r
caffeoyl-CoA + isocitrate
-
486247
Amaranthus paniculatus L.
CoA + 2-caffeoylisocitrate
-
-
-
r
caffeoyl-CoA + isocitric acid
-
486247
Amaranthus hybridus subsp. cruentus
CoA + (E)-caffeoylisocitric acid
-
486247
Amaranthus hybridus subsp. cruentus
r
feruloyl-CoA + isocitric acid
-
486247
Amaranthus hybridus subsp. cruentus
CoA + feruloylisocitric acid
-
486247
Amaranthus hybridus subsp. cruentus
r
additional information
citric, malic tartaric and quinic acids are not accepted as substrates
486247
Amaranthus hybridus subsp. cruentus
?
-
-
-
-
sinapoyl-CoA + isocitric acid
minor activity compared to caffeoyl-CoA
486247
Amaranthus hybridus subsp. cruentus
CoA + sinapoylisocitric acid
-
486247
Amaranthus hybridus subsp. cruentus
r
Other publictions for EC 2.3.1.126
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
486247
Strack
-
Hydroxycinnamic acid esters of ...
Amaranthus hybridus, Amaranthus hybridus subsp. cruentus, Amaranthus paniculatus, Amaranthus paniculatus L., Amaranthus tricolor
Phytochemistry
26
2919-2923
1987
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6
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5
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5
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1
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3
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1
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
5
-
-
-
1
-
3
-
1
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-