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Literature summary for 2.3.1.122 extracted from
- Purification and characterization of a novel mycolic acid exchange enzyme from Mycobacterium smegmatis (1987), J. Biol. Chem., 262, 13417-13423.
General Stability
| General Stability | Organism |
|---|---|
| buffer of ionic strength of 0.1 M and above stabilizes | Mycolicibacterium smegmatis |
| dithiothreitol, 1 mM, stabilizes | Mycolicibacterium smegmatis |
| purification has to be carried out at 4°C | Mycolicibacterium smegmatis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ACP | 0.00056 mg/0.6 ml, 68% reduction of activity | Mycolicibacterium smegmatis |  |
| iodoacetamide | 16.7 mM, 53% decrease of activity | Mycolicibacterium smegmatis | |
| MgCl2 | 16 mM, 91% inhibition | Mycolicibacterium smegmatis | |
| Tween 80 | strong, might disrupt necessary structure of the TM vesicle/micelle | Mycolicibacterium smegmatis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0083 | - |
trehalose | - |
Mycolicibacterium smegmatis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
SDS-PAGE, gel filtration | Mycolicibacterium smegmatis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 alpha,alpha-trehalose 6-mycolate | Mycolicibacterium smegmatis | - |
alpha,alpha-trehalose + alpha,alpha-trehalose 6,6'-bismycolate | - |
r | |
| alpha,alpha-trehalose 6-mycolate + alpha,alpha-trehalose | Mycolicibacterium smegmatis | - |
alpha,alpha-trehalose + alpha,alpha-trehalose 6-mycolate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium smegmatis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate fractionation, gel filtration and Sephadex column chromatography | Mycolicibacterium smegmatis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00779 | - |
- |
Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 alpha,alpha-trehalose 6-mycolate | - |
Mycolicibacterium smegmatis | alpha,alpha-trehalose + alpha,alpha-trehalose 6,6'-bismycolate | - |
r | |
| alpha,alpha-trehalose 6-mycolate + alpha,alpha-trehalose | - |
Mycolicibacterium smegmatis | alpha,alpha-trehalose + alpha,alpha-trehalose 6-mycolate | - |
r | |
| alpha,alpha-trehalose 6-palmitate + trehalose | - |
Mycolicibacterium smegmatis | alpha,alpha-trehalose + alpha,alpha-trehalose 6-palmitate | - |
r | |
| additional information | exchange of mycolyl group, highly specific for alpha,alpha'-trehalose as the mycolate acceptor, less specific for the acyl donor group | Mycolicibacterium smegmatis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 25000, SDS-PAGE | Mycolicibacterium smegmatis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Mycolicibacterium smegmatis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 55 | 75% of maximal activity at 25°C, 12% at 55°C | Mycolicibacterium smegmatis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7 | - |
Mycolicibacterium smegmatis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | 45% of maximal activity at pH 5.0, 30% of maximal activity at pH 9.0 | Mycolicibacterium smegmatis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement of cofactors such as ATP or CoA | Mycolicibacterium smegmatis |
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