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Literature summary for 2.3.1.118 extracted from

  • Summerscales, J.E.; Josephy, P.D.
    Human acetyl CoA:arylamine N-acetyltransferase variants generated by random mutagenesis (2004), Mol. Pharmacol., 65, 220-226.
    View publication on PubMed
Search for more literature for 2.3.1.118

Protein Variants

Protein Variants Comment Organism
I238T about 30% of wild-type activity Homo sapiens
K185N about 40% of wild-type activity Homo sapiens
N172I about 20% of wild-type activity Homo sapiens
N245I enzymic activity comparable to wild-type, slight increase in Km-value Homo sapiens
W77R about 25% of wild-type activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.238
-
 2-Aminofluorene wild-type, pH 7.2, 37°C Homo sapiens
0.298
-
 2-Aminofluorene mutant I238T, pH 7.2, 37°C Homo sapiens
0.301
-
 2-Aminofluorene mutant N245I, pH 7.2, 37°C Homo sapiens
0.304
-
 2-Aminofluorene mutant W77R, pH 7.2, 37°C Homo sapiens
0.367
-
 2-Aminofluorene mutant K185N, pH 7.2, 37°C Homo sapiens
0.857
-
 2-Aminofluorene mutant N172I, pH 7.2, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 isoform NAT2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 2-aminofluorene
-
 Homo sapiens CoA + N-acetyl-2-aminofluorene
-
 ?